Antibacterial activity of bacteriocin-like substance P34 on Listeria monocytogenes in chicken sausage

Sant’Anna, Voltaire; Quadros, Deoni Aparecida Ferreira de; Motta, Amanda de Souza da; Brandelli, Adriano

doi:10.1590/s1517-83822014005000004

Cited by 21 publications

(19 citation statements)

References 20 publications

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“…This study showed that nisin produced larger inhibitory halos than the peptide P34 against the strain of L. seeligeri, although both substances were inhibitory to the Listeria strains tested. Our results for the antimicrobial activity were similar to a previously published study [37]. A significant change in the gene expression level was considered when a three-fold decreased or increased expression was obtained in comparison to control.…”

Section: Resultssupporting

confidence: 89%

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

Vaucher

Giongo²,

Estivales

et al. 2016

Int J Pharm Pharm Sci

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Objective: Investigate the influence of the antimicrobial peptides P34 and nisin on the expression of genes associated with components of the cell surface of Listeria monocytogenes and Listeria seeligeri.Methods: Antimicrobial activity was determined by addition of peptide P34 and nisin (12.5 µg/ml) onto Brain Heart Infusion agar (BHI) plates previously inoculated with indicator strains (L. monocytogenes ATCC 7644 or L. seeligeri AC 82/4) after incubation for 24 h at 37 °C or 240 h at 4 °C. Ribonucleic acid (RNA) was directly extracted from bacterial colonies at the border of the inhibition zones, and the expression levels of genes Dalanine-D-alanyl carrier protein ligase (dltA), putative phospholipid lysinylation (Imo 1695) and EIIAB Man Results: A non-significant increase in the levels of transcription of genes dltA, Imo1695 and mptA was observed for L. monocytogenes treated with peptide P34 or nisin. Both peptides caused a similar decrease in dltA gene expression in L. seeligeri. The expression of gene Imo1695 significantly decreased (about 2000-fold) after treatment with the peptide P34 at 37 °C, while at 4 °C a reduction of 12-fold and 5-fold was detected for P34 and nisin, respectively. A significant decrease in mptA gene expression was observed by exposition to peptide P34 (31.872-fold) and nisin (16.047-fold) for 24 h at 37 °C.of mannose-specific PTS (mptA) were determined using real-time PCR. Conclusion:The results suggest that both peptide P34 and nisin influence the expression of genes related with the cell-surface/cell-membrane structure of L. seeligeri and in lesser extent L. monocytogenes.

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Section: Resultssupporting

confidence: 89%

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

Vaucher

Giongo²,

Estivales

et al. 2016

Int J Pharm Pharm Sci

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“…Sant′Anna et al . () also evidenced reduction, but not eradication, of viable cell counts of L. monocytogenes when the bacteriocin‐like substance P34 was tested in chicken sausage. Similar results were also described in relation to L. monocytogenes inhibition by cerein 8A in dairy products (Bizani et al .…”

Section: Discussionmentioning

confidence: 95%

The antimicrobial peptide aureocin A53 as an alternative agent for biopreservation of dairy products

et al. 2016

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“…These results are somewhat equivalent from that in the literature, once several bacteriocins show better synergism when used in combination with other antimicrobials, including nisin that had potentiated action in combinations with other antimicrobials such as chemical preservatives, phenolic compounds, other natural antimicrobial proteins and organic acid (Franklin, Cooksey, & Getty, 2004;Sant'Anna et al, 2013;Figueiredo & Almeida, 2017). The effectiveness of bacteriocins is often dependent upon environmental factors such as pH and temperature, interactions with food components, precipitation, preparation, inactivation, or uneven distribution of bacteriocin in the food matrix, and food microbiota (Grisi & Gorlach, 2005;Figueiredo & Almeida, 2017).…”

Section: Biopreservative Of Ready-to-eat Pork Hamsupporting

confidence: 78%

Optimization of the yield of bacteriocin-like substance (BLIS) produced by <i>Pediococcus pentosaceus</i> and its application as food bioconservative

Azevedo¹

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Sucrose and inulin were investigated in this study as stimulating agents of bacteriocin production by Pediococcus pentosaceus ATCC 43200 when they were combined with glucose. When such a microbial strain was grown in glucose-based MRS medium, without additional supplements, it reached higher maximum cell concentration (2.68 g/L) and generation time (2.17 h), but lower specific growth rate (0.32 h-1) than in the same medium supplemented with 1.0% of both ingredients (2.53 g/L, 1.60 h and 0.43 h-1 , respectively). Glucose replacement by sucrose or inulin almost completely suppressed growth, hence confirming that it is the preferred carbon source. Qualitatively, similar results were observed for lactate production, which was 59.8% higher in glucose-based medium. Enterococcus and Listeria strains were sensitive to bacteriocin, and both supplements in the glucose-based MRS medium improved the antimicrobial effect against these strains, and were also able to speed up P. pentosaceus in the exponential phase.

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Antibacterial activity of bacteriocin-like substance P34 on Listeria monocytogenes in chicken sausage

Cited by 21 publications

References 20 publications

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

Influence of Peptide P34 on Gene Expression of Listeria Monocytogenes and Listeria Seelegeri

The antimicrobial peptide aureocin A53 as an alternative agent for biopreservation of dairy products

Optimization of the yield of bacteriocin-like substance (BLIS) produced by <i>Pediococcus pentosaceus</i> and its application as food bioconservative

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