2014
DOI: 10.1590/s1517-83822014000400013
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Abstract: The plant growth-promoting rhizobacterial strain Pseudomonas putida Rs-198 was isolated from salinized soils from Xinjiang Province. We optimized the composition of the low-cost medium of P. putida Rs-198 based on its bacterial concentration, as well as its phosphate-dissolving and indole acetic acid (IAA)-producing capabilities using the response surface methodology (RSM), and a mathematical model was developed to show the effect of each medium component and its interactions on phosphate dissolution and IAA p… Show more

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Cited by 21 publications
(16 citation statements)
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“…In addition, multivariable experiments in the RSM reduce the number of optimizations required and provide more accurate results than single‐variable strategies, resulting in significant improvements in enzyme production . Therefore, the optimization of fermentation process parameters and medium components by statistical approaches, such as the Plackett–Burman design and the RSM, has been well appreciated for its significant improvements in activity and reduction in enzyme production costs . Therefore, the culture conditions of high recombinant xylanase under deep fermentation conditions were optimized by statistical experimental design.…”
Section: Introductionmentioning
confidence: 99%
“…In addition, multivariable experiments in the RSM reduce the number of optimizations required and provide more accurate results than single‐variable strategies, resulting in significant improvements in enzyme production . Therefore, the optimization of fermentation process parameters and medium components by statistical approaches, such as the Plackett–Burman design and the RSM, has been well appreciated for its significant improvements in activity and reduction in enzyme production costs . Therefore, the culture conditions of high recombinant xylanase under deep fermentation conditions were optimized by statistical experimental design.…”
Section: Introductionmentioning
confidence: 99%
“…Meanwhile, the soluble phosphate precipitated into its insoluble state. Similarly, Peng et al [ 32 ] reported that P. putida Rs-198 exhibiting phosphate-solubilizing ability led to varied P concentration ranging from 65 μ g·mL −1 to 118 μ g·mL −1 in the medium at stationary growth phase. Mardad et al [ 31 ] isolated Enterobacter hormaechei subsp.…”
Section: Discussionmentioning
confidence: 98%
“…The optimized medium developed in this study (containing crude glycerol, CSL, and feed-grade L-tryptophan) was more cost-effective than other media and achieved high IAA production with a less-expensive fermentation medium. Several low-cost substrates for IAA production have been studied, such as agroindustrial residues, agrowaste substrates, jatropha seedcake, and sweet whey, but the cost of using these substrates has not yet been estimated [40][41][42][43][44] . As shown in Table 2, the fermentation costs of using the initial and optimized medium in the 100-L bioreactor were compared.…”
Section: Discussionmentioning
confidence: 99%
“…The results from the medium optimized by RSM ultimately provided a high IAA yield that was 3.3-fold higher than that of the non-optimized medium. RSM is a statistical optimization method that has been successfully applied by many researchers to improve IAA production 21,24,44,45 . RSM allows more factors to be evaluated at the same time for their effects on IAA production than the OFAT approach and provides less variability in the experiments.…”
Section: Discussionmentioning
confidence: 99%