Characterization of Bacteriocin like inhibitory substance produced by a new Strain Brevibacillus borstelensis AG1 Isolated from 'Marcha'

Sharma, Nivedita; Gupta, A. K.; Gautam, Neha

doi:10.1590/s1517-83822014000300033

Cited by 18 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Brevibacillus borstelensis is a thermophilic bacteria and was isolated from Kalajoha with a similarity of 100%. It was found previously in, ’Marcha’- a herbal cake used as traditional starter culture to ferment local wine in north-east India and rhizosphere of wheat from northern region of India [25, 26]. But it has not been earlier reported as an endophyte of rice.…”

Section: Discussionmentioning

confidence: 99%

Seed interior microbiome of rice genotypes indigenous to three agroecosystems of Indo-Burma biodiversity hotspot

et al. 2019

View full text Add to dashboard Cite

BackgroundSeeds of plants are a confirmation of their next generation and come associated with a unique microbia community. Vertical transmission of this microbiota signifies the importance of these organisms for a healthy seedling and thus a healthier next generation for both symbionts. Seed endophytic bacterial community composition is guided by plant genotype and many environmental factors. In north-east India, within a narrow geographical region, several indigenous rice genotypes are cultivated across broad agroecosystems having standing water in fields ranging from 0-2 m during their peak growth stage. Here we tried to trap the effect of rice genotypes and agroecosystems where they are cultivated on the rice seed microbiota. We used culturable and metagenomics approaches to explore the seed endophytic bacterial diversity of seven rice genotypes (8 replicate hills) grown across three agroecosystems.ResultsFrom seven growth media, 16 different species of culturable EB were isolated. A predictive metabolic pathway analysis of the EB showed the presence of many plant growth promoting traits such as siroheme synthesis, nitrate reduction, phosphate acquisition, etc. Vitamin B12 biosynthesis restricted to bacteria and archaea; pathways were also detected in the EB of two landraces. Analysis of 522,134 filtered metagenomic sequencing reads obtained from seed samples (n=56) gave 4061 OTUs. Alpha diversity indices showed significant differences in observed OTU richness (P≤0.05) across genotypes. Significant differences were also found between the individual hills of a rice genotype. PCoA analysis exhibited three separate clusters and revealed the clusters separated based on genotype, while agroecosystem showed a minimal effect on the variation of seed microbiota (adonis, R2=0.07, P=0.024). Interestingly, animal gut resident bacteria such as Bifidobacterium, Faecalibacterium, Lactobacillus, etc. were found in abundance as members of the seed microbiota.ConclusionOverall, our study demonstrates, indigenous rice genotypes of north-east India have a unique blend of endophytic bacteria in their mature seeds. While there are notable variations among plants of the same genotype, we found similarities among genotypes cultivated in completely different environmental conditions. The beta diversity variations across the seven rice genotypes were significantly shaped by their genotype rather than their agroecosystems.

show abstract

Section: Discussionmentioning

confidence: 99%

Seed interior microbiome of rice genotypes indigenous to three agroecosystems of Indo-Burma biodiversity hotspot

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Lee et al ( 2011a ) identified an anti- C. perfringens lantibiotic produced by Bifidobacterium longum that had a broad range of inhibition. Sharma and colleagues identified a strain of Brevibacillus borstelensis with anti- C. perfringens activity that was associated with a thermostable bacteriocin-like inhibitory substance (BLIS) of 12 kDa, which was active under the physiological conditions expected in the GI tract (Sharma et al, 2014 ).…”

Section: Molecules Of Microbial Originmentioning

confidence: 99%

Alternatives to Antibiotics to Prevent Necrotic Enteritis in Broiler Chickens: A Microbiologist's Perspective

et al. 2015

View full text Add to dashboard Cite

Since the 2006 European ban on the use of antibiotics as growth promoters in animal feed, numerous studies have been published describing alternative strategies to prevent diseases in animals. A particular focus has been on prevention of necrotic enteritis in poultry caused by Clostridium perfringens by the use of microbes or microbe-derived products. Microbes produce a plethora of molecules with antimicrobial properties and they can also have beneficial effects through interactions with their host. Here we review recent developments in novel preventive treatments against C. perfringens-induced necrotic enteritis in broiler chickens that employ yeasts, bacteria and bacteriophages or secondary metabolites and other microbial products in disease control.

show abstract

“…B. borstelensis M9-18 and R. pycnus M9-19 both reduced the mycelial growth of five pathogens (but not P. sulcatum) (Table 4). To the best of our knowledge this is the first report that these bacteria have antagonistic activities against plant pathogens, however it has been reported previously that a strain of B. borstelensis was inhibitory to foodborne microorganisms that include Listeria monocytogenes, B. subtilis, Clostridium perfringens, Staphylococcus aureus, and others (Sharma et al, 2014). P. favisporus did not inhibit any of the tested plant pathogens in our test conditions.…”

Section: Evaluation Of Various Bacterial Antagonists Through General contrasting

confidence: 63%

Distribution of antimicrobial lipopeptides in Bacillus and Pseudomonas spp., two genera with antagonistic effects against plant pathogens

Mohamed¹

View full text Add to dashboard Cite

Suppressive soils, composts, and compost teas have previously shown inhibitory effects against plant disease. A major reason for this suppressiveness has been their beneficial microbial populations. The present study was carried out to investigate the inhibitory activity of forty bacteria isolated from these three sources against the mycelial growth of six plant pathogens.Thirty-eight isolates inhibited at least one of the pathogens whereas sixteen of the isolates inhibited all the pathogens (36% average inhibition). Crude lipopeptides extracts were precipitated from bacterial liquid cultures. All tested lipopeptide samples inhibited the mycelial growth or conidial germination of at least one of the pathogens. Known antimicrobial lipopeptides in selected samples were identified by LC-MS. Results showed that all Bacillus and Bacillus-related spp. produced one or more lipopeptides from the fengycin, iturin, and surfactin families. In addition, certain Pseudomonas spp. produced lipopeptides from the amphisin and putisolvin families.iii Acknowledgments It is with immense gratitude that I acknowledge the support and help of my supervisor, Dr. Tyler Avis. He has been the pivotal point of my academic growth. Dr. Avis' passion and positive attitude towards learning and teaching science has encouraged my educational aspirations. I sincerely thank him for allowing me to pursue research in his lab for five years.

show abstract

Characterization of Bacteriocin like inhibitory substance produced by a new Strain Brevibacillus borstelensis AG1 Isolated from 'Marcha'

Cited by 18 publications

References 20 publications

Seed interior microbiome of rice genotypes indigenous to three agroecosystems of Indo-Burma biodiversity hotspot

Seed interior microbiome of rice genotypes indigenous to three agroecosystems of Indo-Burma biodiversity hotspot

Alternatives to Antibiotics to Prevent Necrotic Enteritis in Broiler Chickens: A Microbiologist's Perspective

Distribution of antimicrobial lipopeptides in Bacillus and Pseudomonas spp., two genera with antagonistic effects against plant pathogens

Contact Info

Product

Resources

About