Concomitant production of detergent compatible enzymes by Bacillus flexus XJU-1

Niyonzima, François N.; More, Sunil S.

doi:10.1590/s1517-83822014000300020

Cited by 31 publications

(44 citation statements)

References 10 publications

(21 reference statements)

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“…Because of the high value of the alkaline proteases, many new data regarding the alkaline protease producing Bacillus strains have been publishing during the last years (Niyonzima and More, 2014;Sari et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

Tekin

Cihan

Karaca

et al. 2017

Polish Journal of Microbiology

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Alkaline proteases have biotechnological importance due to their activity and stability at alkaline pH. 56 bacteria, capable of growing under alkaline conditions were isolated and their alkaline protease activities were carried out at different parameters to determine their optimum alkaline protease production conditions. Seven isolates were showed higher alkaline protease production capacity than the reference strains. The highest alkaline protease producing isolates (103125 U/g), E114 and C265, were identified as Bacillus licheniformis with 99.4% and Bacillus mojavensis 99.8% based on 16S rRNA gene sequence similarities, respectively. Interestingly, the isolates identified as Bacillus safensis were also found to be high alkaline protease producing strains. Genotypic characterizations of the isolates were also determined by using a wide range of molecular techniques (ARDRA, ITS-PCR, (GTG)5-PCR, BOX-PCR). These different techniques allowed us to differentiate the alkaliphilic isolates and the results were in concurrence with phylogenetic analyses of the 16S rRNA genes. While ITS-PCR provided the highest correlation with 16S rRNA groups, (GTG)5-PCR showed the highest differentiation at species and intra-species level. In this study, each of the biotechnologically valuable alkaline protease producing isolates was grouped into their taxonomic positions with multi-genotypic analyses.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

Tekin

Cihan

Karaca

et al. 2017

Polish Journal of Microbiology

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“…The analysis of variance (ANOVA) of the PB design ( regulating the synthesis of hydrolases [29]. Sharma et al, (2012) [30] showed that yeast extract was the main nitrogen source for lipase production by B. licheniformis MTCC-10498.…”

Section: Resultsmentioning

confidence: 99%

Response Surface Methodology (RSM) for analysing culture conditions of Acidocella facilis strain USBA-GBX-505 and Partial Purification and Biochemical Characterization of Lipase 505 LIP

et al. 2017

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Using Response Surface Methodology (RSM) we evaluated the culture conditions (nitrogen source, carbon source, pH and agitation rate) that increase the biomass of Acidocella facilis strain USBA-GBX-505 and therefore enhance the production of its lipolytic enzyme, 505 LIP. RSM results revealed that yeast extract and agitation were key culture factors that increased the growth-associated lipolytic activity by 4.5-fold (from 0.13 U.mg -1 to 0.6 U.mg -1). The 505 LIP lipase was partially purified using size-exclusion chromatography and ion-exchange chromatography. Its molecular weight was >77 kDa. The enzyme shows its optimum catalytic activity at 55 °C and pH 7.5. EDTA, PMSF, 1-butanol and DMSO inhibited enzymatic activity, whereas Tween 20, acetone, glycerol and methanol increased it. Metallic ions are not required for the activity of 505 LIP, and even have an inhibitory effect on the enzyme. This study shows the potential use of A. facilis strain USBA-GBX-505 for the production of a newly identified lipolytic enzyme, 505 LIP, which is stable at moderate temperatures and in the presence of organic solvents. These are important characteristics for the synthesis of many useful products.

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“…B. flexus has been used for production of enzymes, e.g. β‐amylase in food production and of alkaline amylase, lipase and protease for detergent formulations (Niyonzima and More, ). It has also been used for the production of exopolysaccharides (EPS) (Singh et al., ), and for bioremediation (Sivaprakasam et al., ; Pal et al., ; Das et al., ).…”

Section: Assessmentmentioning

confidence: 99%

Scientific Opinion on the update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA†

Ricci¹,

Allende²,

Bolton³

et al. 2017

EFS2

217

130

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EFSA is requested to assess the safety of a broad range of biological agents in the context of notification for market authorisation as sources of food and feed additives, food enzymes and plant protection products. The qualified presumption of safety (QPS) assessment was developed to provide a harmonised generic preassessment to support safety risk assessments performed by EFSA's scientific Panels. The safety of unambiguously defined biological agents (at the highest taxonomic unit appropriate for the purpose for which an application is intended), and the completeness of the body of knowledge are assessed. Identified safety concerns for a taxonomic unit are, where possible and reasonable in number, reflected as 'qualifications' in connection with a recommendation for a QPS status. The list of QPS recommended biological agents was reviewed and updated in the current opinion and therefore becomes the valid list. The 2016 update reviews previously assessed microorganisms including bacteria, yeasts and viruses used for plant protection purposes following an Extensive Literature Search strategy. The taxonomic units related to the new notifications received since the 2013 QPS opinion, were periodically evaluated for a QPS status and the results published as Statements of the BIOHAZ Panel. Carnobacterium divergens, Lactobacillus diolivorans, Microbacterium imperiale, Pasteuria nishizawae, Pediococcus parvulus, Bacillus flexus, Bacillus smithii, Xanthomonas campestris and Candida cylindracea were recommended for the QPS list. All taxonomic units previously recommended for the 2013 QPS list had their status reconfirmed as well their qualifications with the exception of Pasteuria nishizawae for which the qualification was removed. The exclusion of filamentous fungi and enterococci from the QPS evaluations was reconsidered but monitoring will be maintained and the status will be re-evaluated in the next QPS Opinion update. Evaluation of bacteriophages should remain as a case-by-case procedure and should not be considered for QPS status. Acknowledgements: The BIOHAZ Panel wishes to thank the EFSA staff member: Mirena Ivanova for the support provided to this scientific output.

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Concomitant production of detergent compatible enzymes by Bacillus flexus XJU-1

Cited by 31 publications

References 10 publications

Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

Response Surface Methodology (RSM) for analysing culture conditions of Acidocella facilis strain USBA-GBX-505 and Partial Purification and Biochemical Characterization of Lipase 505 LIP

Scientific Opinion on the update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA†

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