Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region

Abstract

Two native Pleurotus spp. strains (white LB-050 and pale pink LB-051) were isolated from rotten tree trunks of cazahuate (Ipomoea murucoides) from the Mexican Mixtec Region. Both strains were chemically dedikaryotized to obtain their symmetrical monokaryotic components (neohaplonts). This was achieved employing homogenization time periods from 60 to 65 s, and 3 day incubation at 28 °C in a peptone-glucose solution (PGS). Pairing of compatible neohaplonts resulted in 56 hybrid strains which were classified into the four following hybrid types: (R1-nxB1-n, R1-nxB2-1, R2-nxB1-n and R2-nxB2-1). The mycelial growth of Pleurotus spp. monokaryotic and dikaryotic strains showed differences in texture (cottony or floccose), growth (scarce, regular or abundant), density (high, regular or low), and pigmentation (off-white, white or pale pink). To determine the rate and the amount of mycelium growth in malt extract agar at 28 °C, the diameter of the colony was measured every 24 h until the Petri dish was completely colonized. A linear model had the best fit to the mycelial growth kinetics. A direct relationship between mycelial morphology and growth rate was observed. Cottony mycelium presented significantly higher growth rates (p < 0.01) in comparison with floccose mycelium. Thus, mycelial morphology can be used as criterion to select which pairs must be used for optimizing compatible-mating studies. Hybrids resulting from cottony neohaplonts maintained the characteristically high growth rates of their parental strains with the hybrid R1-nxB1-n being faster than the latter.

Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models

“…After that, the Petri dishes were observed under the microscope 10(x) to identify the micelyum without clamp connections (neohaplonts). Finally the monokaryotic components were cultivated individually on Petri dishes with 10 mL of MEA to verify under the microscope 10(x) the absence of clamp connections (Guadarrama-Mendoza et al, 2014).…”

Propolis: An Enrichment Material for Mycelium Development of Oyster Mushroom (&lt;i&gt;Pleurotus ostreatus&lt;/i&gt;)

“…Mushrooms get their food from the environment by means of mycelia. Mycelial growth rate, obtaining good quality spawns and product contamination is among important factors affecting productivity [21]. Propolis, as an enrichment material to growth media, may help to overcome such problems and improve productivity by promoting mycelial development as well as preventing it from contamination.…”

Growth Assessment of Six Wild Syrian Oyster Mushroom Strains (Pleurotus ostreatus) on Three Nutrient Media

“…The media were autoclaved at 121°C for 20 minutes then 25 ml were poured into 90x15 mm sterile Petri dishes (Ishaq et al, 2017). The media were left to solidify, then incubated at 25°C for 24h for sterility testing (Guadarrama-Mendoza et al, 2014).…”

“…Three dishes were used for each strain and medium. The dishes were incubated in darkness at 25°C till the mycelium of most strains reached the edges of the Petri dishes (8 days) ( Gibriel et al, 1996;Nasim et al, 2001;Guadarrama-Mendoza et al, 2014).…”