Optimization of biodegradable plastic production on sugar cane molasses in Enterobacter sp. SEL2

Abstract

Contaminated environments have a large number of bacteria which can accumulate PHA as their energy reserves. Out of 54 isolated bacterial strains from three groups of contaminated sites 48 were found PHA positive. The sites were grouped on the basis of the type of carbon sources i.e. sugars, fatty acids and much diverse type. Strains MFD5, MFD11, UML3, USL2, SEL2, SEL3, SEL10 and PFW1 produced 69.9 ± 0.29, 75.27 ± 0.45, 65.43 ± 0.1, 72.54 ± 0.27, 76.61 ± 0.28, 61.81 ± 0.05, 71.16 ± 0.09 and 74.92 ± 0.5 percent of PHA to their constant cell weight (CCW) respectively in PHA detection media supplemented with 2% glucose. Molasses, whey, crumbs hydrolysate and palm oil were checked as inexpensive carbon sources. Molasses alone could supply the required nutrients for growth and PHA production. Strain SEL2 produced 47.36 ± 0.45% PHA using 2% molasses at 37 °C and pH 7.0. Upon production optimization the best accumulation (80.95 ± 0.01%) was observed in PHA detection media with 0.2% nitrogen source, 3% molasses, pH 5.0 and 37 °C by the strain SEL2. The overall effect of the presence of increased molasses concentration in the media was positive it increased the accumulation period till 72 h. Enterobacter sp. SEL2 (JF901810) is first time being reported for PHA production.

Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses

“…In another study, Naheed and Jamil obtained a maximum PHA yield of 80.5% dcw by Enterobacter sp. SEL2, a bacterium isolated from soil of contaminated sites, with the use 3% of sugarcane molasses after fermentation for 24 h at pH 5.0 [25]. Chaijamrus and Udpuay reported a 43% yield of P(3HB) by Bacillus megaterium ATCC 6748 after 45 h of fermentation when 4% sugarcane molasses was used as the carbon source [26].…”

PHB Production in Biofermentors Assisted through Biosensor Applications

“…The possibility to use plant byproducts such as sugar cane molasses as growth media made the use of naturally producing strains economically convenient [41,8]. These authors used agroindustrial wastes for PHB production but adding them as supplementary carbon source in synthetic media such as LB or PCA, together with additional fructose, glucose or ammonium sulphate.…”

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

“…[326] Sinorhizobium meliloti 41 and Hydrogenophaga pseudoflava DSM 1034 can biosynthesize P(3HB), P(3HB-co-3HV) copolyesters, and P(3HB-co-3HV-co-4HB) terpolyesters from lactose. [329][330][331] Furthermore, PHAs can also be produced directly from molasses (e.g.,s ugarcane, sugar beet,a nd soy molasses), ahigh sucrose content byproduct from the sugar industry,o ro ther sucrose-rich products (e.g.,m aple sap, sugar beet juice, pineapple juice, or oil palm frondj uice) by using C. necator, [332] A. vinelandii, [331] Enterobacter species, [333] Pseudomonas corrugate, [334] A. latus, [335] recombinant E. coli, [336,337] B. subtilis, [337] B. megaterium, [338] Bacillus cereus, [339] or mixed cultures. [327] Sucrose, ad isaccharide composed of ag lucosea nd af ructose unit, is one of the most abundant and relativelyi nexpensive carbon sources extracted from sugar-bearing raw materials, such as sugar beet and sugarcane.…”