Utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using Aspergillus niger GH1

Abstract

Agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. Molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. Invertase is an important enzyme for sweeteners development. In this study, a xerophilic fungus Aspergillus niger GH1 isolated of the Mexican semi-desert, previously reported as an invertase over-producer strain was used. Molasses from Mexico and Cuba were chemically analyzed (total and reducer sugars, nitrogen and phosphorous contents); the last one was selected based on chemical composition. Fermentations were performed using virgin and hydrolyzate bagasse (treatment with concentrated sulfuric acid). Results indicated that, the enzymatic yield (5231 U/L) is higher than those reported by other A. niger strains under solid state fermentation, using hydrolyzate bagasse. The acid hydrolysis promotes availability of fermentable sugars. In addition, maximum invertase activity was detected at 24 h using low substrate concentration, which may reduce production costs. This study presents an alternative method for invertase production using a xerophilic fungus isolated from Mexican semi-desert and inexpensive substrates (molasses and sugarcane bagasse).

The potential application of fungus Trichoderma harzianum Rifai in biodegradation of detergent and industry

“…and Aspergillus sp. has been reported [30][31][32]. According to Poonawalla et al [33], the fungus P. chrysogenum has both extracellular and intracellular invertase, which are distinguished by their subcellular localization (cell wall, vacuole or cytosol), solubility (soluble or insoluble in low ionic strength buffer), optimum pH (acid or neutral/alkaline) and isoelectric point (pI).…”

Comparative evaluation of extracellular β‐d‐fructofuranosidase in submerged and solid‐state fermentation produced by newly identifiedBacillus subtilisstrain

“…; Veana et al . ). The nature of the substrates in a fermentation medium is crucial for enzyme productivity.…”

“…It possesses a rich content of sucrose and accounts up to 60% of total sugars (Veana et al . ). Solid substrates such as bran, husk (rice, wheat), corn, sugarcane bagasse, cassava, fruit peels, vegetable wastes and even spent matter have been investigated for invertase production from various species of yeasts and some moulds (Rashad and Nooman ; Alves et al .…”

Solid-State Culture for Lignocellulases Production

“…and T. versicolor solid-state monocultures was determined by quantifying diferent activities: xylanases, oxido-reductases and cellulases, since it is known that these enzymatic families are involved in invasion of lignocellulosic material [23]. Furthermore, due to wheat bran and sugar cane bagasse composition [24], amylase and invertase activities were also measured.…”