Detection of enteric viruses in activated sludge by feasible concentration methods

Prado, Tatiana; Gaspar, Ana; Miagostovich, Marize Pereira

doi:10.1590/s1517-83822014000100049

Cited by 33 publications

(12 citation statements)

References 29 publications

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“…The magnitude of viral loss in this study is, however, in accordance with those of other studies on virus detection in wastewater (21,22,33). In addition, the concentration seemed to have removed inhibitors of qPCR present in raw sewage, as has been shown also by others (21,22,33,34).…”

Section: Discussionsupporting

confidence: 92%

Detection of Pathogenic Viruses in Sewage Provided Early Warnings of Hepatitis A Virus and Norovirus Outbreaks

Hellmér

Paxéus

Magnius

et al. 2014

Appl Environ Microbiol

384

281

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dMost persons infected with enterically transmitted viruses shed large amounts of virus in feces for days or weeks, both before and after onset of symptoms. Therefore, viruses causing gastroenteritis may be detected in wastewater, even if only a few persons are infected. In this study, the presence of eight pathogenic viruses (norovirus, astrovirus, rotavirus, adenovirus, Aichi virus, parechovirus, hepatitis A virus [HAV], and hepatitis E virus) was investigated in sewage to explore whether their identification could be used as an early warning of outbreaks. Samples of the untreated sewage were collected in proportion to flow at Ryaverket, Gothenburg, Sweden. Daily samples collected during every second week between January and May 2013 were pooled and analyzed for detection of viruses by concentration through adsorption to milk proteins and PCR. The largest amount of noroviruses was detected in sewage 2 to 3 weeks before most patients were diagnosed with this infection in Gothenburg. The other viruses were detected at lower levels. HAV was detected between weeks 5 and 13, and partial sequencing of the structural VP1protein identified three different strains. Two strains were involved in an ongoing outbreak in Scandinavia and were also identified in samples from patients with acute hepatitis A in Gothenburg during spring of 2013. The third strain was unique and was not detected in any patient sample. The method used may thus be a tool to detect incipient outbreaks of these viruses and provide early warning before the causative pathogens have been recognized in health care.

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Section: Discussionsupporting

confidence: 92%

Detection of Pathogenic Viruses in Sewage Provided Early Warnings of Hepatitis A Virus and Norovirus Outbreaks

Hellmér

Paxéus

Magnius

et al. 2014

Appl Environ Microbiol

384

281

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“…Two studies from Tunisia and China, found RV in 72•4% and 93.5% of sewage samples using real time PCR, respectively [6,26]. Moreover, our detection rates of RV in sewage sludge is lower than those detected in study from Brazil [26].…”

Section: Discussioncontrasting

confidence: 58%

Molecular Detection and Genotyping of Group A Rotavirus by Multiplex Semi-Nested RT-PCR in Sewage Water and Sludge

Shaheen¹

2019

OAJMB

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Group A rotavirus (RV-A) infections are a major cause of morbidity and mortality in human around the world. The aim of this research was to conduct a molecular characterization of RV-A glycoprotein (G) and protease-sensitive (P) in urban sewage (n=54; 27 raw sewage and 27 treated sewage) and sewage sludge (n=27) from Egypt by multiplex semi-nested RT-PCR method. RV-A was detected in 29.9% of raw sewage, 7.4% of treated sewage, and 18.5% of sewage sludge samples. In the positive samples, the RV-A G genotypes were as follow: G3 (n=6), G9 (n=3), G1 (n=2), G4 (n=1), G10 (n=1), G1+G3 (n =1), and G1+G3+G10 (n=1) whereas P types detected included: P[4] (n=1), P[6] (n=3), P[8] (n=8), and nontypeable P (n=3). The most detected G-P combination was G3P [8] (n=6). Other G-P combinations such as G1P [8] (n=2), G9P [6] (n=2), G4P [6] (n=1), and G10P[4] (n=1) were also detected. The highest detection rates of RV-A in sewage samples were found in winter (50%), followed by summer (25%), then in spring and autumn (12.5%). The results showed not only that sewage analysis contains dramatic information concerning enteric viruses, but also that environmental monitoring is an important approach in describing the local circulation of specific viruses among population.

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“…However, both methods resulted in less than 7.5% mean recovery of HAdV, Rotavirus A (RoV-A), NoV GII and HAV from the sludge. These results suggested that the applied concentration methods were unable to reduce the presence of the inhibitors that affected the subsequent PCR-based detections ( Prado et al, 2014 ).…”

Section: Methods Of Pre-treatment Of Wastewater Samplesmentioning

confidence: 99%

Viruses in wastewater: occurrence, abundance and detection methods

Corpuz

Buonerba

Vigliottá

et al. 2020

Science of The Total Environment

182

160

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This paper presents an updated and comprehensive review on the different methods used for detection and quantification of viruses in wastewater treatment systems. The analysis of viability of viruses in wastewater and sludge is another thrust of this review. Recent studies have mostly focused on determining the abundance and diversity of viruses in wastewater influents, in samples from primary, secondary, and tertiary treatment stages, and in final effluents. A few studies have also examined the occurrence and diversity of viruses in raw and digested sludge samples. Recent efforts to improve efficiency of virus detection and quantification methods in the complex wastewater and sludge matrices are highlighted in this review. A summary and a detailed comparison of the pre-treatment methods that have been utilized for wastewater and sludge samples are also presented. The role of metagenomics or sequencing analysis in monitoring wastewater systems to predict disease outbreaks, to conduct public health surveillance, to assess the efficiency of existing treatment systems in virus removal, and to re-evaluate current regulations regarding pathogenic viruses in wastewater is discussed in this paper. Challenges and future perspectives in the detection of viruses, including emerging and newly emerged viruses such as the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), in wastewater systems are discussed in this review.

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Detection of enteric viruses in activated sludge by feasible concentration methods

Cited by 33 publications

References 29 publications

Detection of Pathogenic Viruses in Sewage Provided Early Warnings of Hepatitis A Virus and Norovirus Outbreaks

Detection of Pathogenic Viruses in Sewage Provided Early Warnings of Hepatitis A Virus and Norovirus Outbreaks

Molecular Detection and Genotyping of Group A Rotavirus by Multiplex Semi-Nested RT-PCR in Sewage Water and Sludge

Viruses in wastewater: occurrence, abundance and detection methods

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