Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates

De-sheng, Meng; Sun, Changjian; Yu, Jingbo; Jun, Ma; Xue, Wei

doi:10.1590/s1517-83822014000100034

Cited by 18 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Depending on the antibiotic, significant mutations can occur in the genes of certain enzymes [ 5 ]. For example, development of in vitro resistance to pefloxacin, ofloxacin, ciprofloxacin, and trovafloxacin in Mycoplasma hominis is associated with mutations in the topoisomerase IV gene, while resistance to sparfloxacin occurs due to mutations in the DNA gyrase gene [ 5 , 41 , 56 ]. Fluoroquinolone-resistant clinical isolates of mycoplasma usually demonstrate cross-resistance to all drugs of this group.…”

Section: Discussionmentioning

confidence: 99%

“…Efforts to figure out the causes of increased fluoroquinolone resistance by microorganisms, which are currently being reported all over the world [ 56 , 59 , 60 ], have led to the assumption that, in addition to these mechanisms, there are other ways that determine the possibility of rapid bacterial adaptation to antibiotics in microbial communities [ 55 ]. This assumption is based on the results of both experimental studies and monitoring data in different countries.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mycoplasmas and Their Antibiotic Resistance: The Problems and Prospects in Controlling Infections

et al. 2016

View full text Add to dashboard Cite

The present review discusses the problem of controlling mycoplasmas (class Mollicutes), the smallest of self-replicating prokaryotes, parasites of higher eukaryotes, and main contaminants of cell cultures and vaccines. Possible mechanisms for the rapid development of resistance to antimicrobial drugs in mycoplasmas have been analyzed. Omics technologies provide new opportunities for investigating the molecular basis of bacterial adaptation to stress factors and identifying resistomes, the total of all genes and their products contributing to antibiotic resistance in microbes. The data obtained using an integrated approach with post-genomics methods show that antibiotic resistance may be caused by more complex processes than has been believed heretofore. The development of antibiotic resistance in mycoplasmas is associated with essential changes in the genome, proteome, and secretome profiles, which involve many genes and proteins related to fundamental cellular processes and virulence.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mycoplasmas and Their Antibiotic Resistance: The Problems and Prospects in Controlling Infections

et al. 2016

View full text Add to dashboard Cite

show abstract

“…[20]. Многочисленные однонуклеотидные замены в генах gyrA, gyrB, parC и parЕ изолятов M. hominis являют-ся причиной высокого генетического полиморфизма и играют ведущую роль в формировании антибиотикорезистентности, что согласуется с данными [21][22][23].…”

Section: экспериментальная частьunclassified

The genome structure of ciprofloxacin-resistant Mycoplasma hominis clinical isolates

Kolesnikova¹,

Бруснигина²,

Makhova³

et al. 2020

Acta Naturae

View full text Add to dashboard Cite

The genome structure of three ciprofloxacin-resistant Mycoplasma hominis clinical isolates was studied using next-generation sequencing on the Illumina platform. The protein sequences of the studied Mycoplasma strains were found to have a high degree of homology. Mycoplasma hominis (M45, M57, MH1866) was shown to have limited biosynthetic capabilities, associated with the predominance of the genes encoding the proteins involved in catabolic processes. Multiple single-nucleotide substitutions causing intraspecific polymorphism of Mycoplasma hominis were found. The genes encoding the efflux systems ABC transporters (the ATP-binding cassette superfamily) and proteins of the MATE (multidrug and toxic compound extrusion) family were identified. The molecular mechanism of ciprofloxacin resistance of the Mycoplasma hominis M45 and M57 isolates was found to be associated with the Ser83Leu substitution in DNA gyrase subunit A. In the Mycoplasma hominis MH1866 isolate it was related to the Lys144Arg substitution in topoisomerase IV subunit A.

show abstract

“…This is due to topo-isomerase IV gene mutation. Due to mutation of the gene coding for DNA gyrase, sparfloxacin resistance is observed 44,139 .…”

Section: Antimycoplasma Antibiotic Resistancementioning

confidence: 99%

Emerging Antibiotic Resistance in Mycoplasma Microorganisms, Designing Effective and Novel Drugs / Therapeutic Targets: Current Knowledge and Futuristic Prospects

Yatoo¹,

Parray²,

Bhat³

et al. 2019

J Pure Appl Microbiol

View full text Add to dashboard Cite

Emerging antibiotic resistance among mycoplasma microorganisms is of major concern in present times as they cause various diseases in both animals and humans. Mycoplasmoses, infections caused by mycoplasma microorganisms have become common in recent past and have gained importance both due to inability to diagnose and difficulty to treat. Respiratory tract infection, mastitis, arthritis, and septicemia caused by Mycoplasma in livestock are responsible for causing heavy economic losses. These diseases are frequently reported from countries of Africa and Asia, including India. Antimycoplasma antibiotics are frequently being used as therapeutic agents for the treatment of mycoplasmoses infection in livestock. They include macrolides, tetracyclines, fluoroquinolones, and aminoglycosides which are the main antibiotic classes commonly used against mycoplasma globally. Oxytetracyclines are the commonest antibiotics used for decades followed by enrofloxacin, tylosin, and streptomycin. Danofloxacin, lincomycin, spiramycin, erythromycin, gamithromycin, azithromycin, clarithromycin, gentamicin, doxycycline, and tulathromycin are also used occasionally. Continuous and unregulated use of these antibiotics over prolonged period can lead to menace of antibiotic resistance which is aided by inappropriate doses and uncontrolled use. Resistance to some antibiotics is already emerging. Mycoplasmas have devised different resistance mechanisms for combating antimicrobial action of these drugs. Common mechanisms noted are acquisitions of proteins affecting ribosomal subunits, inhibition of antibiotic efflux, structural changes in the ribosomal subunit, target mutations, expression or production of enzymes. Additional novel mechanisms of resistance still need to be investigated. Strategies for prevention and encountering of this antibiotic resistance are being devised by alternating antibiotics in application, using antimycoplasma antibiotic sensitivity tests, along with evaluation of specific doses and exploration of novel mycoplasma specific class of antibiotics. Novel targets based on various cell structures including cell membrane, organelles, proteins, enzymes or metabolites are being explored for antimycoplasma therapy. These all will help in effective therapeutic management of mycoplasmoses with minimal side effects.

show abstract

Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates

Cited by 18 publications

References 20 publications

Mycoplasmas and Their Antibiotic Resistance: The Problems and Prospects in Controlling Infections

Mycoplasmas and Their Antibiotic Resistance: The Problems and Prospects in Controlling Infections

The genome structure of ciprofloxacin-resistant Mycoplasma hominis clinical isolates

Emerging Antibiotic Resistance in Mycoplasma Microorganisms, Designing Effective and Novel Drugs / Therapeutic Targets: Current Knowledge and Futuristic Prospects

Contact Info

Product

Resources

About