Screening of endoglucanase-producing bacteria in the saline rhizosphere of Rhizophora mangle

Sá, André Luís Braghini; Dias, Armando Cavalcante Franco; Quecine, Maria Carolina; Cotta, Simone Raposo; Fasanella, Cristiane Cipola; Andreote, Fernando Dini; Melo, I. S. de

doi:10.1590/s1517-83822014000100025

Cited by 7 publications

(2 citation statements)

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“…The structure and composition of microbial communities in mangrove are influenced by several factors such as nutrient content, biogeography, depth, anthropogenic activities, and tidal regime [8][9][10][11]. In recent years, the microbial communities of mangroves have been assessed using a range of techniques, including classical cultivation approach, fingerprinting techniques, clone libraries and next generation sequencing [8,9,11,12]. These studies focused on the diversity and distribution of microbial communities at different sites [8,10], different points within a mangrove [8], state of preservation [9,11,13], contamination by oil spill [14] and shrimp farms [15], and depths [11].…”

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confidence: 99%

Variations of Bacterial Community Structure and Composition in Mangrove Sediment at Different Depths in Southeastern Brazil

Mendes

Tsai

2014

Diversity

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Tropical mangroves are considered one of the most productive ecosystems of the world, being characterized as nurseries and food sources for fish and other animals. Microorganisms play important roles in these environments, and the study of bacterial communities is of paramount importance for a better comprehension of mangrove dynamics. This study focused on the structure and composition of bacterial communities in mangrove sediments at different depths and points, located in Southeastern Brazil. Terminal Restriction Fragment Length Polymorphism (T-RFLP) was used to determine the community structure, and 16S rRNA gene pyrosequencing was used to characterize the community composition. Redundancy analysis of T-RFLP patterns revealed differences in bacterial community structure according to soil attributes and depth. The parameters K and depth presented significant correlation with general community structure. Most sequences were classified into the phylum Proteobacteria (88%), which presented differences according to the depth, where the classes Betaproteobacteria (21%) and Deltaproteobacteria (16%) were abundant at 10 cm and Epsilonproteobacteria (35%) was abundant at 40 cm depth. Clear differences were observed in community composition as shown by the differential distribution of the phyla Firmicutes (1.13% and 3.8%, for 10 cm and 40 cm respectively), Chloroflexi (2.8% and 0.75%), and Acidobacteria (2.75% and 0.57%) according to the depth. Bacterial diversity measurements indicated higher diversity in shallow samples. Taken together, our findings indicate that mangrove holds a diverse bacterial community, which is shaped by the variations found in the ecosystem, such as sediment properties and depth.

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Section: Introductionmentioning

confidence: 99%

Variations of Bacterial Community Structure and Composition in Mangrove Sediment at Different Depths in Southeastern Brazil

Mendes

Tsai

2014

Diversity

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“…In the world (mainly especially in developing countries), toxic strain E.coli is responsible for about 2.5 million infant deaths per year (Kosek et al, 2003). Consequently, the study of bacteria, especially coliform (total coliform and fecal coliform) in soil and water environment was carried out in many countries in the world (Mendes et al, 2012;Dias et al, 2010;Sá et al, 2014;Nguyen et al, 2014...).…”

Section: Introductionmentioning

confidence: 99%

Microbial contamination in the surface water in the Ba Vi National Park, Ha Noi

Le¹,

Phung

2018

J. Viet. Env.

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Ba Vi National Park, one of 28 Vietnamese National Parks, is currently preserved and exploited for a variety of purposes, including the preservation of intact natural forest ecosystems and genetic resources of rare plants and animals. This paper presents the monitoring results of microbial contamination in surface water environment of the Ba Vi National Park (Ha Noi) in the period 2013-2014 and 2018. The results showed that total coliform (TC) density varied from 23 to 11,000 MPN/100ml in bimonthly observation in 2013-2014 which was lower than that one of sampling campaign in 2018, from 900 MPN/100ml to 8,100 MPN/ml. Fecal coliform (FC) densities varied from 0 to 110 MPN/100ml in 2013-2014, lower than in 2018, when it varied from 0 MPN/100ml to 600 MPN/100ml. At several observation times, both TC and FC were higher than the allowable values of the Vietnam national technical regulation QCVN 08-MT: 2015/BTNMT column A1 for surface water quality. The exceeded values of TC and FC than the allowable values and the increase trend from the 2013 to 2018 periods indicated the potential risks to the public health in this region when people use water for domestic and agricultural purposes. Our results provide dataset for environmental management in the Ba Vi National Park in order to protect the eco-environment in parallel with social-economic development. Vườn Quốc gia Ba Vì, một trong 28 vườn quốc gia của Việt Nam hiện đang được bảo tồn và khai thác cho nhiều mục đích, trong đó có bảo tồn nguyên vẹn các hệ sinh thái rừng tự nhiên, các nguồn gen động, thực vật quý hiếm, các đặc sản rừng và các di tích lịch sử, cảnh quan tự nhiên trong vùng. Bài báo trình bày kết quả quan trắc, đánh giá mức độ ô nhiễm vi sinh vật trong môi trường nước mặt tại Vườn Quốc Gia Ba Vì, Hà Nội giai đoạn 2013-2014 và 2018. Kết quả cho thấy mật độ coliform tổng số (TC) biến đổi trong khoảng từ 23 – 11.000 MPN/100ml trong các đợt quan trắc định kỳ 2 tháng/lần trong hai năm 2013 - 2014 và trong khoảng từ 900 – 8.100 MPN/100ml trong một đợt quan trắc năm 2018. Mật độ fecal coliform (FC) biến đổi trong khoảng từ 0 - 110 MPN/100ml năm 2013 -2014 và từ 0 - 600 MPN/100ml vào năm 2018. Vào một số thời điểm quan trắc, mật độ FC và TC vượt giá trị cho phép của quy chuẩn kỹ thuật Quốc Gia QCVN 08-MT:2015/BTNMT cột A1 về chất lượng nước mặt. Các giá trị TC và FC vượt quá giá trị cho phép và xu hướng gia tăng TC và FC từ 2013 -2018 cho thấy nguy cơ tiềm tàng khi người dân sử dụng nguồn nước này cho các mục đích sinh hoạt và nông nghiệp. Như vậy, kết quả của nghiên cứu này nhấn mạnh nhu cầu giám sát thường xuyên chất lượng nước và cần thực hiện các giải pháp hiệu quả để xử lý và quản lý nguồn gây ô nhiễm trong khu vực nhằm bảo vệ môi trường sinh thái song song với phát triển kinh tế - xã hội của khu vực.

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Mangrove soil as a source for novel xylanase and amylase as determined by cultivation-dependent and cultivation-independent methods

et al. 2019

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Xylanase and α-amylase enzymes participate in the degradation of organic matter, acting in hemicellulose and starch mineralization, respectively, and are in high demand for industrial use. Mangroves represent a promising source for bioprospecting enzymes due to their unique characteristics, such as fluctuations in oxic/anoxic conditions and salinity. In this context, the present work aimed to bioprospect xylanases from mangrove soil using cultivation-dependent and cultivation-independent methods. Through screening from a metagenomic library, three potentially xylanolytic clones were obtained and sequenced, and reads were assembled into contigs and annotated. The contig MgrBr135 was affiliated with the Planctomycetaceae family and was one of 30 ORFs selected for subcloning that demonstrated only amylase activity. Through the cultivation method, 38 bacterial isolates with xylanolytic activity were isolated. Isolate 11 showed an enzymatic index of 10.9 using the plate assay method. Isolate 39 achieved an enzyme activity of 0.43 U/mL using the colorimetric method with 3,5-dinitrosalicylic acid. Isolate 39 produced xylanase on culture medium with salinity ranging from 1.25 to 5%. Partial 16S rRNA gene sequencing identified isolates in the Bacillus and Paenibacillus genera. The results of this study highlight the importance of mangroves as an enzyme source and show that bacterial groups can be used for starch and hemicellulose degradation.

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Screening of endoglucanase-producing bacteria in the saline rhizosphere of Rhizophora mangle

Cited by 7 publications

References 19 publications

Variations of Bacterial Community Structure and Composition in Mangrove Sediment at Different Depths in Southeastern Brazil

Variations of Bacterial Community Structure and Composition in Mangrove Sediment at Different Depths in Southeastern Brazil

Microbial contamination in the surface water in the Ba Vi National Park, Ha Noi

Mangrove soil as a source for novel xylanase and amylase as determined by cultivation-dependent and cultivation-independent methods

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