2014
DOI: 10.1590/s1517-83822014000100023
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Abstract: Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA… Show more

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Cited by 33 publications
(25 citation statements)
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“…The number of OTUs per phylotype is shown in brackets. used (Ranjard et al, 2003;Kang and Mills, 2006;Zhao and Xu, 2012;Leite et al, 2014). Therefore, we recommend investigating the sample size factor prior to any extensive environmental microbial community analysis.…”
Section: Discussionmentioning
confidence: 99%
“…The number of OTUs per phylotype is shown in brackets. used (Ranjard et al, 2003;Kang and Mills, 2006;Zhao and Xu, 2012;Leite et al, 2014). Therefore, we recommend investigating the sample size factor prior to any extensive environmental microbial community analysis.…”
Section: Discussionmentioning
confidence: 99%
“…The use of reproducible and efficient strategies for DNA extraction is essential for most protocols in molecular biology analyses (10,17). In this study, we tried to evaluate different methods to find the most efficient, economic and performable way associated with the acceptable purity of the extracted DNA from L. casei.…”
Section: Discussionmentioning
confidence: 99%
“…These methods can vary due to the efficiency of physical and chemical characteristics of samples (17). In the current work, we used one type of bacterium in the same cultivating condition and time.…”
Section: Discussionmentioning
confidence: 99%
“…Several Brazilian universities use the technique for various studies. Researchers at UNIOESTE and Unipar evaluated the effects of using wastewater to irrigate crops 134 , comparing the effects of cover crops, evaluated by UFSC 135 , the comparison between different types of management, conducted by UFU 136 and UNB, Embrapa Cerrado and UFRRJ 107 , comparison of farming systems conducted by UEL, UEM and Embrapa Soja 137 .The UFRJ together with Embrapa Solos and Embrapa Arroz e Feijão, also used the DGGE technique to evaluate treatment using biochar, which provides a reduction in CO2 emissions by agriculture and the promotion of plants growing 138 .Assessments of ecology and soil dynamics or water in the case of irrigated crops, combine different views on agriculture in search of lower costs to farmers and also, lower environmental impact. …”
Section: Perspectivesmentioning
confidence: 99%