Antibiotic resistance in lactic acid bacteria isolated from some pharmaceutical and dairy products

Abstract

A total of 244 lactic acid bacteria (LAB) strains were isolated from 180 dairy and pharmaceutical products that were collected from different areas in Minia governorate, Egypt. LAB were identified phenotypically on basis of morphological, physiological and biochemical characteristics. Lactobacillus isolates were further confirmed using PCR-based assay. By combination of phenotypic with molecular identification Lactobacillus spp. were found to be the dominant genus (138, 76.7%) followed by Streptococcus spp. (65, 36.1%) and Lactococcus spp. (27, 15%). Some contaminant organisms such as (Staphylococcus spp., Escherichia coli, Salmonella spp., mould and yeast) were isolated from the collected dairy samples but pharmaceutical products were free of such contaminants. Susceptibility of LAB isolates to antibiotics representing all major classes was tested by agar dilution method. Generally, LAB were highly susceptible to Beta-lactams except penicillin. Lactobacilli were resistant to vancomycin, however lactococci and streptococci proved to be very susceptible. Most strains were susceptible to tetracycline and showed a wide range of streptomycin MICs. The MICs of erythromycin and clindamycin for most of the LAB were within the normal range of susceptibility. Sixteen Lactobacillus, 8 Lactococcus and 8 Streptococcus isolates including all tetracycline and/or erythromycin resistant strains were tested for the presence of tetracycline and/or erythromycin resistant genes [tet(M) and/or erm(B)]. PCR assays shows that some resistant strains harbor tet(M) and/or erm(B) resistance genes.

Identification and characterization of tetracycline resistance in Lactococcus lactis isolated from Polish raw milk and fermented artisanal products

“…These results overlap with the results reported by other research groups, indicating that most of the antibioticresistant LAB strains are resistant to tetracycline and erythromycin (Devirgiliis et al, 2010). Similar results were obtained for both the bacteria isolated from the human digestive system (Mitsou et al, 2010) and as the bacteria isolated from dairy products Florez et al, 2005Florez et al, , 2007Gad et al, 2014;Nawaz et al, 2011). The reason for this may be the huge amount of tetracyclines that have been used for years in agriculture livestock farming as "growth promoters".…”

Gut Microbiota Modulation and Its Relationship with Obesity Using Prebiotic Fibers and Probiotics: A Review

“…None of the studies have analyzed the comparative effect of different strains with regard to their anti-obesity potential, so the issue certainly requires further research. The biggest one is the safety issue of probiotics, although they are known to be safe for human consumption, but at the same time we cannot deny from the fact that they may spread antibiotic resistance (Gad et al, 2014) Likewise, some probiotics could also cause gastrointestinal disorders as previously discussed in a review (Marteau and Seksik, 2004). This reflects the need for a stricter regulatory framework globally.…”

Antibiotic resistance patterns of lactic acid bacteria isolated from Nigerian grown salad vegetables

“…The occurrence of Lactobacillus spp. in largest number is in tandem with various reports (Tamang et al, 2005;Gomes et al, 2010;Gad et al, 2014). Salad vegetables are served and eaten raw in Nigeria and indeed many other climes as such, any AR borne on conjugative mobile genetic elements poses a great danger to the populace.…”