Characterization of a virulent Leptospira interrogans strain isolated from an abandoned swimming pool

Forster, Karine M.; Hartwig, Daiane Drawanz; Seixas, Fabiana Kömmling; McBride, Alan J. A.; Monte, Leonardo Garcia; Recuero, Ana Lúcia Coelho; Brod, Claudiomar Soares; Hartleben, Cláudia Pinho; Amaral, Marta; Dellagostin, Odir A.

doi:10.1590/s1517-83822013005000029

Cited by 10 publications

(13 citation statements)

References 18 publications

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“…Blood samples were collected from the retroorbital venous plexus before each immunization and challenge, and the sera were stored at Ϫ20°C. Forty-two days after the first immunization, the hamsters were challenged intraperitoneally with a dose of 10 leptospires, equivalent to 5 times the 50% lethal dose (LD 50 ) (19,31), of the L. interrogans serogroup Icterohaemorrhagiae strain Spool (32). Hamsters were monitored daily and were euthanized upon the appearance of clinical symptoms of leptospirosis.…”

Section: Methodsmentioning

confidence: 99%

A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

Hartwig

Forster

Oliveira

et al. 2013

Clin. Vaccine Immunol.

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bToward developing an effective vaccine capable of conferring heterologous protection, the putative lipoprotein LemA, which presents an M3 epitope similar to that of Listeria, was evaluated as a vaccine candidate in the hamster model of leptospirosis. LemA is conserved (>70% pairwise identity) among the pathogenic Leptospira spp., indicating its potential in stimulating a cross-protective immune response. Using different vaccination strategies, including prime-boost, DNA vaccine, and a subunit preparation, recombinant LemA conferred different levels of protection in hamsters. Significant protection against mortality was observed for the prime-boost and the DNA vaccine strategies, which showed 87.5% (P < 0.01) and 62.5% (P < 0.05) efficacy, respectively. Although the subunit vaccine preparation protected 50.0% of immunized hamsters, the level of protection was not significant. None of the hamsters in the control groups survived challenge with a virulent strain of Leptospira interrogans serogroup Icterohaemorrhagiae. Characterization of the immune response found that the strongest antibody response was stimulated by the subunit vaccine preparation, followed by the prime-boost strategy. The DNA vaccine failed to elicit an antibody response in immunized hamsters.

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Section: Methodsmentioning

confidence: 99%

A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

Hartwig

Forster

Oliveira

et al. 2013

Clin. Vaccine Immunol.

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“…Our group characterised this isolate and found that it was highly virulent in the hamster model, causing lesions that are typically associated with severe leptospirosis, with an LD 50 of ~2 leptospires. This indicated that L. interrogans Piscina may pose a risk to public health in the region ( Forster et al 2013 ). Due to its high virulence, this isolate has also been used as a challenge strain in experiments aimed to evaluate the protection afforded by novel recombinant vaccine candidates against leptospirosis in vaccinology laboratories ( Forster et al 2015 ).…”

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confidence: 99%

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

Jorge

Kremer

Oliveira

et al. 2018

Mem. Inst. Oswaldo Cruz

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BACKGROUNDLeptospirosis is the most widespread zoonotic disease. It is caused by infection with pathogenic Leptospira species, of which over 300 serovars have been described. The accurate identification of the causative Leptospira spp. is required to ascertain the pathogenic status of the local isolates.OBJECTIVESThis study aimed to obtain the complete genome sequence of a virulent Leptospira interrogans strain isolated from southern Brazil and to describe its genetic features.METHODSThe whole genome was sequenced by next-generation sequencing (Ion Torrent). The genome was assembled, scaffolded, annotated, and manually reviewed. Mutations were identified based on a variant calling analysis using the genome of L. interrogans strain Fiocruz L1-130 as a reference.FINDINGSThe entire genome had an average GC content of 35%. The variant calling analysis identified 119 single nucleotide polymorphisms (SNPs), from which 30 led to a missense mutation. The structural analyses identified potential evidence of genomic inversions, translocations, and deletions in both the chromosomes.MAIN CONCLUSIONSThe genome properties provide comprehensive information about the local isolates of Leptospira spp., and thereby, could facilitate the identification of new targets for the development of diagnostic kits and vaccines.

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“…A wide variety of susceptible animals can host this serovar (FORSTER et al, 2013;LANGONI et al, 2016;RODRIGUES et al, 2015). Maciel et al (2008) investigated seropositivity to the Copenhageni serovar in 269 residents of peripheral neighborhoods in the city of Salvador in Bahia, Brazil, and found that 30% were seropositive, with titers ranging from 25 to 400.…”

Section: Discussionmentioning

confidence: 99%

A serological survey of agents causing leptospirosis and toxoplasmosis in Rattus rattus in the city of Umuarama, northwest Paraná, Brazil

Lopes

Germano

Gerônimo

et al. 2017

SCA

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Synanthropic rodents, present in both urban and rural areas, are responsible for the zoonotic transmission of several diseases to humans as well as for significant economic losses. They act as reservoirs for important viral diseases, bacterial diseases such as leptospirosis, and parasitic diseases such as toxoplasmosis and leishmaniasis. The aim of the present study was to assess the seropositivity of synanthropic rodents in Umuarama city, located in the northwestern region of Paraná State, Brazil, to agents causing leptospirosis and toxoplasmosis. The microscopic agglutination technique (MAT) was used to detect anti-Leptospira antibodies, and the indirect immunofluorescence reaction (IIFR) was used to detect anti-Toxoplasma gondii antibodies. Of 178 animals collected, four (2.24%) were seropositive for Leptospira spp. and ten (5.62%) for Toxoplasma gondii. Ninety-five (53.38%) of the collected animals were male and 83 (46.62%) were female, and two (1.23%) originated from urban areas while 176 (98.87%) originated from peri-urban areas. Serological results showed that the synanthropic rodents examined here had low seroreactivity for agents causing both leptospirosis and toxoplasmosis, in both urban and peri-urban regions of Umuarama city. This could be associated with the high Human Development Index for the study area. However, preventative measures must continue to be observed, as rodents are important reservoirs for, and disseminators of, disease causing agents. ResumoOs roedores sinantrópicos determinam grandes prejuízos econômicos, e são responsáveis pela transmissão de várias zoonoses ao homem tanto em áreas urbanas quanto nas rurais, atuando como reservatórios de importantes doenças que incluem a leptospirose, toxoplasmose, leishmaniose e algumas de etiologia viral. O presente trabalho investigou a sororeatividade para leptospirose e toxoplasmose em roedores sinantrópicos da cidade de Umuarama, cidade localizada na região noroeste do estado do Paraná, Brasil. A técnica Soroaglutinação Microscópica (SAM) foi utilizada para a detecção de anticorpos anti-Leptospira spp e a Reação de Imunofluorescência Indireta (RIFI) para a detecção de anticorpos anti-Toxoplasma gondii. Dos 178 animais examinados, quatro (2,24%) foram reagentes para Leptospira spp. e 10 (5,62%) para o Toxoplasma gondii. Destes animais, 95 (53,38%) eram machos e 83 (46,62%) fêmeas, dois (1,23%) eram provenientes da área urbana e 176 (98,87%) da área periurbana. Os resultados obtidos demonstraram que os roedores sinantrópicos examinados apresentaram baixa freqüência de sororeatividade para as duas zoonoses investigadas, tanto na região urbana como na periurbana da cidade de Umuarama, fato que pode estar associado ao alto Índice de Desenvolvimento Humano encontrado, contudo, as medidas preventivas devem ser mantidas, pois os roedores são importantes elos na cadeia epidemiológica das doenças analisadas.

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Characterization of a virulent Leptospira interrogans strain isolated from an abandoned swimming pool

Cited by 10 publications

References 18 publications

A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

A Prime-Boost Strategy Using the Novel Vaccine Candidate, LemA, Protects Hamsters against Leptospirosis

Whole-genome sequencing of Leptospira interrogans from southern Brazil: genetic features of a highly virulent strain

A serological survey of agents causing leptospirosis and toxoplasmosis in Rattus rattus in the city of Umuarama, northwest Paraná, Brazil

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