Computation of interactive effects and optimization of process parameters for alkaline lipase production by mutant strain of Pseudomonas aeruginosa using response surface methodology

Bisht, Deepali; Yadav, Santosh Kumar; Darmwal, N. S.

doi:10.1590/s1517-83822013005000016

Cited by 12 publications

(5 citation statements)

References 24 publications

(20 reference statements)

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“…Immobilized cells prepared by the above methods were transferred to 250-mL Erlenmeyer flasks containing 50 mL of production medium, obtained after the optimization using RSM (Box-Behnken method) containing (in g/L): starch, 15; castor oil, 1.77; yeast extract, 0.2; (NH 4 ) 2 SO 4 , 1.0; KH 2 PO 4, 0.6; MgSO 4 , 0.4; Triton-X-100, 0.93, and gum arabic, 5.0; with pH 8.1 (16). The flasks were incubated on a rotary shaker (120 rpm) at 34.1 °C for 48 h. For the free cell culture, 50 mL of production medium was inoculated with bacterial cells equivalent to those used for immobilization.…”

Section: Screening Of Different Matrices For Immobilization Of Mutant Cells For Lipase Productionmentioning

confidence: 99%

Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level Level

Bisht¹,

Yadav²,

Darmwal³

2013

Turk J Biol

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Suitability of 3 matrices, agarose, sodium alginate, and polyacrylamide, for immobilization of mutant cells of Pseudomonas aeruginosa MTCC 10,055 was investigated. Of these, agarose was proven to be the best as exhibiting maximum enzyme production (4363.4 U/mL), followed by polyacrylamide gel (2172.3 U/mL). Alginate beads were the poorest. The one-variable-at-a-time approach suggested agarose at 2.0%, immobilized bead at 4.0 g blocks/50 mL, and initial cell loading of 0.8 g in the matrix as optimum conditions for maximum lipase production (5982.3 U/mL) after 24 h of incubation. However, response surface methodology studies determined the optimum values of these variables as 1.96%, 4.06 g blocks/50 mL, and 0.81 g of cells in the matrix for maximum lipase production (6354.23 U/mL) within 22.54 h of incubation. The agarose blocks were reusable for 7 cycles without any significant loss in lipase yield. Bench-scale bioreactor level optimization resulted in further enhancement in lipase yield (6815.3 U/mL) at 0.6 vvm aeration and 100 rpm agitation within only 20 h of incubation. Presumably, this is the first attempt for lipase production by immobilized cells of P. aeruginosa at the bioreactor level. The agarose-immobilized mutant cells showed potential candidature for alkaline lipase production at the industrial level.

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Section: Screening Of Different Matrices For Immobilization Of Mutant Cells For Lipase Productionmentioning

confidence: 99%

Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level Level

Bisht¹,

Yadav²,

Darmwal³

2013

Turk J Biol

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“…In their description of the interaction between temperature and pH, they reported that the effect of a high temperature and pH on the amount of lipase production was negative. The highest lipase activity was observed at 35 °C and pH 8.5 [ 20 ]. In contrast to our results, Khoramnia et al showed that temperature plays an important role in lipase production from Acinetobacter sp., while pH had no significant effect in the optimization process [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

Statistical Methodologies for the Optimization of Lipase and Biosurfactant by Ochrobactrum intermedium Strain MZV101 in an Identical Medium for Detergent Applications

2017

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The Plackett–Burman design and the Box–Behnken design, statistical methodologies, were employed for the optimization lipase and biosurfactant production by Ochrobactrum intermedium strain MZV101 in an identical broth medium for detergent applications. Environmental factor pH determined to be most mutual significant variables on production. A high concentration of molasses at high temperature and pH has a negative effect on lipase and biosurfactant production by O. intermedium strain MZV101. The chosen mathematical method of medium optimization was sufficient for improving the industrial production of lipase and biosurfactant by bacteria, which were respectively increased 3.46- and 1.89-fold. The duration of maximum production became 24 h shorter, so it was fast and cost-saving. In conclusion, lipase and biosurfactant production by O. intermedium strain MZV101 in an identical culture medium at pH 10.5–11 and 50–60 °C, with 1 g/L of molasses, seemed to be economical, fast, and effective for the enhancement of yield percentage for use in detergent applications.

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“…The overall observed coefficient of determination (R 2 ) and the percentage of variance provided by the model were 94.59 and 85.0%, respectively, which are appropriate because, in a model well fitted to the experimental data, the value of R 2 should be greater than 80% (BISHT et al, 2013). Therefore, the no significant lack of fit and the high value of R .…”

Section: Resultsmentioning

confidence: 99%

Optimisation of the alcoholic fermentation of aqueous jerivá pulp extract

et al. 2014

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ABSTRACT. The objective of this research is to determinate the optimum conditions for the alcoholic fermentation process of aqueous jerivá pulp extract using the response surface methodology and simplex optimisation technique. The incomplete factorial design 3³ was applied with the yeast extract, NH 4 H 2 PO 4 and yeast as the independent variables and the alcohol production yield as the response. The regression analysis indicated that the model is predictive, and the simplex optimisation generated a formulation containing 0.35 g L NH 4 H 2 PO 4 for an optimum yield of 85.40% ethanol. To validate the predictive equation, the experiment was carried out in triplicate under optimum conditions, and an average yield of 87.15% was obtained. According to a t-test, no significant difference was observed (on the order of 5%) between the average value obtained and the value indicated by the simplex optimisation technique.Keywords: simplex optimisation, experimental design, yeast.Otimização da fermentação alcoólica de extrato aquoso da polpa de jerivá RESUMO. O objetivo deste trabalho foi determinar as melhores condições do processo de fermentação alcoólica do extrato aquoso da polpa de jerivá, utilizando a metodologia de superfície de resposta e otimização simplex. Foi empregado o delineamento fatorial incompleto 3 3 tendo o extrato de levedura, NH 4 H 2 PO 4 e levedura como variáveis independentes e o rendimento da produção de álcool como resposta. A análise da regressão mostrou que o modelo é preditivo e a otimização simplex indicou uma formulação contendo 0,35 g L -1de extrato de levedura, 6,33 g L -1de levedura e 0,30 g L -1de NH 4 H 2 PO 4 para um rendimento ótimo de produção de etanol de 85,40%. Para a validação da equação preditiva o experimento foi conduzido em triplicata, nas condições ótimas estabelecidas, obtendo-se um valor médio de 87,15% de rendimento. Aplicando-se o teste t, verificou-se que não houve diferença significativa, em nível de 5%, entre o valor médio obtido e aquele indicado na otimização simplex.Palavras-chaves: otimização simplex, delineamento experimental, levedura.

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Computation of interactive effects and optimization of process parameters for alkaline lipase production by mutant strain of Pseudomonas aeruginosa using response surface methodology

Cited by 12 publications

References 24 publications

Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level Level

Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level Level

Statistical Methodologies for the Optimization of Lipase and Biosurfactant by Ochrobactrum intermedium Strain MZV101 in an Identical Medium for Detergent Applications

Optimisation of the alcoholic fermentation of aqueous jerivá pulp extract

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