16S rRNA gene-based identification of microbiota associated with the parthenogenetic troglobiont sand fly Deanemyia maruaga (Diptera, Psychodidae) from central Amazon, Brazil

Sousa, Katianne Barbosa Alves de; Silva, Túllio Romão Ribeiro da; Alencar, Ronildo Baiatone; Baton, Luke Anthony; Naveca, Felipe Gomes; Shimabukuro, Paloma Helena Fernandes

doi:10.1590/s1517-83822013005000013

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…Wolbachia has also been reported in the Phlebotominae (Diptera: Psychodidae) both in New (Ono et al ., 2001; Azpurua et al ., 2010) and Old World species (Zhou et al ., 1998). Phlebotomines are vectors of several viral, bacterial and protozoal diseases of humans and other animals, but there are few studies on the presence of Wolbachia in sand flies (Cui et al ., 1999; Ono et al ., 2001; Benlarbi & Ready, 2003; Matsumoto et al , 2008; Azpurua et al ., 2010; de Sousa et al ., 2013) and about the biological relationship of the endosymbiont with the host (Kassem et al , 2003; Kassem & Osman 2007). In Iran, a new strain of Wolbachia was recently found in Phlebotomus perfiliewi transcaucasicus Perfil'ev, 1937 (Parvizi et al ., 2013), increasing the list of phlebotomines known to be infected with this endosymbiont.…”

Section: Discussionmentioning

confidence: 99%

Broader prevalence of Wolbachia in insects including potential human disease vectors

Oliveira¹,

Gonçalves²,

Baton³

et al. 2015

Bull. Entomol. Res.

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Wolbachia are intracellular, maternally transmitted bacteria considered the most abundant endosymbionts found in arthropods. They reproductively manipulate their host in order to increase their chances of being transmitted to the offspring, and currently are being used as a tool to control vector-borne diseases. Studies on distribution of Wolbachia among its arthropod hosts are important both for better understanding why this bacterium is so common, as well as for its potential use as a biological control agent. Here, we studied the incidence of Wolbachia in a broad range of insect species, collected from different regions of Brazil, using three genetic markers (16S rRNA, wsp and ftsZ), which varied in terms of their sensitivity to detect this bacterium. The overall incidence of Wolbachia among species belonging to 58 families and 14 orders was 61.9%. The most common positive insect orders were Coleoptera, Diptera, Hemiptera and Hymenoptera, with Diptera and Hemiptera having the highest numbers of Wolbachia-positive families. They included potential human disease vectors whose infection status has never been reported before. Our study further shows the importance of using quantitative polymerase chain reaction for high-throughput and sensitive Wolbachia screening.

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