Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02

Abstract

The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.

Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa

“…Polysaccharides are widely distributed in animal, plants and microorganisms, which is a large natural resource ( Tao et al, 2016 , Ma et al, 2013 , Atta et al, 2017 ). Recently, considerable interest has arisen in characterizing the polysaccharide of mushrooms, which show various pharmacological functions, including anti-tumor, immunoregulatory and antioxidant activities ( Du et al, 2016 , Palanisamy et al, 2016 , Tian et al, 2016 , Sun et al, 2013 ). Polysaccharides from Agrocybe cylindracea SL-02 had enhancement effects on the reducing power of reactive oxygen species ( Liu et al, 2016b ).…”

Bioactivity assessment of exopolysaccharides produced by Pleurotus pulmonarius in submerged culture with different agro-waste residues

“…EPS produced by Gomphidius rutilus , mutant Cordyceps militaris SU5-08 and Pleurotus spp. in submerged fermentation demonstrated pronounced antioxidant effects on superoxide anion, 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radical, and reducing power ( Gao et al., 2012 ; Lin et al., 2012 ; Sun et al., 2014 ; Bamigboye et al., 2016 ). EPS have different monosaccharide composition with functional groups such as –OH, –CH 3 , –COOH, and –C=O that substantiate its free radical scavenging and metal chelating activities ( Shen et al., 2013 ).…”

Structure characterization and bioactivity of neutral polysaccharides from different sources of Polygonatum Mill

“…Reducing power was determined according to the method reported by Sun et al ., with modifications. [ 30 ] Briefly, 1.0 mL of polysaccharide sample solution at a series of concentrations (1.0, 2.0, 4.0, 6.0, 8.0, 10.0 mg/mL), 0.2 mL of PBS buffer (0.2 mol/L, pH = 6.6) and 0.5 mL potassium ferricyanide (1%, wt/vol) were mixed into each centrifuge tube and incubated at 50 °C for 20 min. After cooling, 1 mL of TCA solution (10%, wt/vol) was poured into the mixture and centrifuged at 4000 r/min for 10 min.…”