2013
DOI: 10.1590/s1517-83822013000300033
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Abstract: This study was evaluated the clonal diversity of Streptococcus mutans in caries-free and caries-active subjects using MLEE. Strains from caries-free subjects were grouped in a single taxon. Unrooted dendrogram showed that different strains clustered in four different clades, also showed that more than one clonal type can be found in a same individual.

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Cited by 11 publications
(13 citation statements)
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References 37 publications
(16 reference statements)
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“…Once again, MLEE proved to be a powerful tool for the typing of S. aureus in epidemiological studies. These results are in agreement with previously reported data on the discriminatory power and reproducibility of the MLEE method as applied to bacteria and yeasts of medical importance [23][24][25][26][27]31 , but the discriminatory power was higher than the values reported for S. aureus by other groups of researchers 29,30 .…”
Section: Discussionsupporting
confidence: 93%
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“…Once again, MLEE proved to be a powerful tool for the typing of S. aureus in epidemiological studies. These results are in agreement with previously reported data on the discriminatory power and reproducibility of the MLEE method as applied to bacteria and yeasts of medical importance [23][24][25][26][27]31 , but the discriminatory power was higher than the values reported for S. aureus by other groups of researchers 29,30 .…”
Section: Discussionsupporting
confidence: 93%
“…Phenotypic methods (biotyping, serotyping, bacteriophage or bacteriocin typing and antimicrobial susceptibility profiles) and genotypic [pulsed-field gel electrophoresis (PFGE) and other methods based on the restriction of genomes, analysis of plasmids, typing methods based on polymerase chain reaction (PCR)] of microbiological characterization have elucidated the relationship and the distribution of human pathogens, which is considered essentially important for the epidemiology and control of hospital infections 17 . Isoenzymatic typing [multilocus enzyme electrophoresis (MLEE)] has been used for several decades as a "gold standard" in population genetics studies of eukaryotes [18][19][20] and systematic studies 21 , as well as in large-scale studies for determining the genetic diversity and structure of natural populations of a variety of bacteria species [22][23][24] and fungi [25][26][27] . This method represents an invaluable complement to the more recently developed molecular typing methods, particularly for largescale epidemiological studies 28 .…”
Section: Introductionmentioning
confidence: 99%
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“…These results suggest that compared with E. coli and Salmonella , Klebsiella possess more capacities to thrive in plant‐associated environments. Further evidence is provided by the fact that Klebsiella have the unique property to use sucrose in its five isomers (Thompson et al ., ) and that Klebsiella strains are selected in tannic acid‐rich environments (Tahmourespour et al ., ). The presence of Klebsiella is well documented in the rhizosphere (Da Costa et al ., ) and in numerous plants, including vegetables (Falomir et al ., ), raising the possibility that people could get colonized and infected through the consumption of contaminated vegetables.…”
Section: Discussionmentioning
confidence: 97%
“…Phenotypic and genotypic methods have allowed researchers to classify isolates of microorganisms in systematic, taxonomic, evolutionary, phylogenetic and epidemiological studies 21 - 23 . Among these methods, multilocus enzyme electrophoresis (MLEE) has been used for several decades as a standard method to study eukaryotic population genetics 24 and systematics 25 as well as large-scale studies to estimate the genetic diversity and structure of natural populations of a variety of bacterial species 26 - 29 and fungal species including yeasts 30 - 33 . Results of different studies, with some exceptions 34 , suggest that relatively few clones of S. aureus are responsible for the majority of intramammary cattle infections with broad geographical distribution 23 , 35 - 37 .…”
Section: Introductionmentioning
confidence: 99%