In situ biosurfactant production and hydrocarbon removal by Pseudomonas putida CB-100 in bioaugmented and biostimulated oil-contaminated soil

Abstract

In situ biosurfactant (rhamnolipid) production by Pseudomonas putida CB-100 was achieved during a bioaugmented and biostimulated treatment to remove hydrocarbons from aged contaminated soil from oil well drilling operations. Rhamnolipid production and contaminant removal were determined for several treatments of irradiated and non-irradiated soils: nutrient addition (nitrogen and phosphorus), P. putida addition, and addition of both (P. putida and nutrients). The results were compared against a control treatment that consisted of adding only sterilized water to the soils. In treatment with native microorganisms (non-irradiated soils) supplemented with P. putida, the removal of total petroleum hydrocarbons (TPH) was 40.6%, the rhamnolipid production was 1.54 mg/kg, and a surface tension of 64 mN/m was observed as well as a negative correlation (R = −0.54; p < 0.019) between TPH concentration (mg/kg) and surface tension (mN/m), When both bacteria and nutrients were involved, TPH levels were lowered to 33.7%, and biosurfactant production and surface tension were 2.03 mg/kg and 67.3 mN/m, respectively. In irradiated soil treated with P. putida, TPH removal was 24.5% with rhamnolipid generation of 1.79 mg/kg and 65.6 mN/m of surface tension, and a correlation between bacterial growth and biosurfactant production (R = −0.64; p < 0.009) was observed. When the nutrients and P. putida were added, TPH removal was 61.1%, 1.85 mg/kg of biosurfactants were produced, and the surface tension was 55.6 mN/m. In summary, in irradiated and non-irradiated soils, in situ rhamnolipid production by P. putida enhanced TPH decontamination of the soil.

A Novel Approach to Enhance Crude Oil Recovery Ratio Using Selected Bacterial Species

“…cyriacigeorgica was reported to degrade aliphatic and branched chain alkanes as well as alkylbenzene of oil in contaminated soils [32], whereas in the current study this isolate showed the ability to biotransform heavy oil with an API gravity value of 26 • , reducing its viscosity and increasing the oil recovery to 7.9%. In a bioaugmentation treatment to remove hydrocarbons, Pseudomonas putida CB-100 enhanced TPH decontamination of contaminated aged soil; 40.6% of total petroleum hydrocarbons (TPH) was removed and 1.54 mg/kg rhamnolipid was reported produced [33]. Our P. putida utilized the heavy fraction of heavy crude oil and gave 61% of biotransformation, a reduction in viscosity from 1.565 Pa.s at 0.7622 s −1 to 0.5415 Pa.s at 98.67 s, and, interestingly, increased the oil recovery by 10.66%.…”

Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

“…Thus, investigating the role of biosurfactant-producing isolate BSP9 in the current study is important for its future utilization in various areas. Although P. putida has been reported to produce different types of biosurfactants, mostly rhamnolipids have been reported [ 55 , 56 , 57 ]. However, the present study reports a strain of P. putida BSP9 with PGP characteristics including phosphate solubilization, production of IAA and siderophore along with biosurfactant.…”

The Role of pH, Electrodes, Surfactants, and Electrolytes in Electrokinetic Remediation of Contaminated Soil

“…Ángeles and Refugio [ 121 ] studied the in situ production of biosurfactants and hydrocarbon removal by Pseudomonas putida CB-100 in bioaugmented and biostimulated oil-contaminated soil. In this research, biosurfactants were produced in biostimulated soil, with the highest biosurfactant yield of 1.88 ± 0.06 mg/kg and 1.97 ± 0.19 for irradiated soil and non-irradiated soil, respectively.…”