Identification and antimicrobial susceptibility patterns of Pasteurella multocida isolated from chickens and japanese quails in Brazil

Rigobelo, Everlon Cid; Blackall, P. J.; Maluta, Renato Pariz; Ávila, Fernando Antônio de

doi:10.1590/s1517-83822013000100023

Cited by 15 publications

(11 citation statements)

References 16 publications

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“…Isolation of P. multocida in the trachea and liver of chicken was earlier reported by Dashe et al [10]. The antibiotic resistance of the P. multocida in this study showed a high resistance to the tested antibiotics compare to reports of previous studies; Everlon et al [11] reported antibiotic resistance of P. multocida to range between 1.5 and 5.2% in isolates from chicken while Dashe et al [10] reported resistance ranging between 6.7 and 46.7%. In other various reports [2], [15], [20] it was found that the resistance level is not as high as found in this research.…”

Section: Discussionsupporting

confidence: 73%

“…Atere et al [5] and Atere [3] reported a high resistant level in the E. coli and Salmonella isolates respectively, which was attributed to misuse of antibiotics before clinical reports. Antimicrobial resistance in P. multocida has been linked to small plasmids [11]. The coexistence and spread of these small plasmids has resulted in P. multocida isolates that are multi-resistant [19].…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis

Victor¹,

Mathew²,

Oluyege³

et al. 2016

Int. J. Sci. World

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Pasteurella multocida is a poultry bacterial pathogen causing fowl cholera in chicken. The prevalence and antibiotic susceptibility of P. multocida isolates from freshly dead chicken were determined. Ninety seven (97) freshly dead chicken from 23 different farms were analyzed for the presence of P. multocida. Swabs of the trachea and the liver of the necropsied chicken were activated on buffered peptone water and later cultured on blood agar and MacConkey agar. Pure culture of organisms were subjected to cultural and biochemical characterization. In vitro susceptibility of the pure isolates of P. multocida against 12 antimicrobial agents was determined using disk diffusion method. Twelve isolates of P. multocida were recovered from the chicken, with a prevalence of 12.4%. Nine of the isolates were recovered from the trachea and three from the liver. All the 12 isolates recovered from the birds were multi-resistant to the antibiotics used in this research. The antibiogram showed that all the isolates resisted ampicillin, amoxicillin/clavulinate, doxycycline and tylosine. Nitrofuratoin and gentamycin had the best antimicrobial activity with 25% and 50% resistance respectively. The resistance of other antibiotics are: Ofloxacin 75%, Ciprofloxacin 83.3%, Enrofloxacin 75%, Furasol 66.7%, Ceftazidime 91.7% and Cefuroxime 66.7%. This result showed that there is an emergence of multi-resistance in P. multocida, therefore it is important to carry out sensitivity test before administration of antibiotics in order to control fowl cholera.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis

Victor¹,

Mathew²,

Oluyege³

et al. 2016

Int. J. Sci. World

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“…One member of this family, Pasteurella multocida, is the causative agent of multiple different diseases that have great economic impact on animal production, including hemorrhagic septicemia in cattle, atrophic rhinitis in swine and fowl cholera (FC) in domesticated and wild birds (Harper et al, 2006;Glisson, 2008;Rigobelo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

Furian

Borges

Pilatti

et al. 2014

Rev. Bras. Cienc. Avic.

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The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. Five different serogroups have been identified based on serological typing. However, such tests are laborious, and agglutination of homologous antiserum may fail. The aim of this study was to develop a multiplex PCR protocol for the identification of the hyaD-hyaC and dcbF genes specific to serogroups A and D, respectively, and to compare these results with those of phenotypic tests for 54 strains isolated from fowl cholera cases in southern Brazil. The kappa coefficient and chisquare statistics were calculated to assess the agreement between the diagnostic methods and to determine the significance of the results, respectively. The multiplex PCR was able to detect the evaluated genes. Forty-nine strains (90.74%) were classified into serogroup A, and only two isolates (3.7%) were not identified as belonging to any of the serogroups analyzed. In contrast, with the phenotypic tests, only 41 strains (75.93%) were classified into serogroup A and 11 samples (20.37%) were unidentifiable. Of the strains analyzed, 70.37% were classified into the same serogroup (A) by both methods, and the kappa coefficient (k = 0.017) indicated poor agreement between the tests. Thus, multiplex PCR is an alternative for P. multocida capsular typing, as it allows the simultaneous and rapid detection of genes and also provides a greater strain-typing capacity.

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“…Antibiotic resistance in bacteria isolated from chicken was reportedly linked to the ability of some of the bacteria to take up plasmid from other resistant bacteria [7]. The coexistence and spread of these small plasmids have resulted in most isolates showing multi-resistant [11].…”

Section: Discussionmentioning

confidence: 99%

Comparative study of antibiotic resistance in bacteria isolated from dog and chicken

Victor¹,

Samuel²,

Folashade³

2017

Int. J. Sci. World

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The emergence of antibiotic resistance has caused a threat to both human and animal population. This research was designed to investigate and compare the antibiotic resistance of bacteria isolated from chicken and dogs. A hundred and twelve samples of freshly dead chicken and eighty nine blood samples of sick dogs were analyzed. Pure culture of isolates were identified using cultural, morphological and biochemical characteristic. In vitro, susceptibility of the identified isolates against antimicrobial agents were determined by the standard disk diffusion procedure. One hundred and six isolates were recovered from chicken while 27 isolates were recovered from dogs. The organisms isolated include E. coli, Haemophilus sp, Pasturella sp, Klebsiella sp, Enterobacter sp, Salmonella sp, Staphylococcus sp., Micrococcus sp., Pseudomonas sp, Proteus sp, and Listeria sp. The antibiotic resistance showed that, gram-negative bacteria showed more resistance to the antibiotics used in this research compare to the gram-positive bacteria. This trend was found in isolates from both dog and chicken. In like manner, the bacteria isolates recovered from chicken showed a greater resistance when compare with the bacteria isolates recovered from dog. The increased resistance found in poultry makes poultry a suspect of residual resistance gene and probably reservoir for transmission.

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Identification and antimicrobial susceptibility patterns of Pasteurella multocida isolated from chickens and japanese quails in Brazil

Cited by 15 publications

References 16 publications

Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis

Prevalence and antibiotic resistance of Pasteurella multocida isolated from chicken in Ado-Ekiti metropolis

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

Comparative study of antibiotic resistance in bacteria isolated from dog and chicken

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