2012
DOI: 10.1590/s1517-83822012000400012
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Abstract: The availability of a useful tool for simple and timely detection of the most important virulent varieties of Escherichia coli is indispensable. To this end, bacterial DNA pools which had previously been categorized were obtained from isolated colonies as well as selected in terms of utilized phenotype; the pools were aatA. This study is an initial step toward carrying out epidemiological field research that will reveal the presence of these bacterial varieties.

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Cited by 5 publications
(4 citation statements)
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“…Genome of ZR1 was extracted by QIAamp DNA mini kit (QIAGEN, Germany) and 2 multiplex polymerase chain reaction (PCR) were conducted to detect virulence genes (eaeA,bfpA,stx1,stx2,ipaH,ST,LT and aatA) of the purified strain with a 16S rRNA PCR as control (Chacon-J. et al, 2012).…”
Section: Isolation and Identification Of Pathogenic E Colimentioning
confidence: 99%
“…Genome of ZR1 was extracted by QIAamp DNA mini kit (QIAGEN, Germany) and 2 multiplex polymerase chain reaction (PCR) were conducted to detect virulence genes (eaeA,bfpA,stx1,stx2,ipaH,ST,LT and aatA) of the purified strain with a 16S rRNA PCR as control (Chacon-J. et al, 2012).…”
Section: Isolation and Identification Of Pathogenic E Colimentioning
confidence: 99%
“…In addition, positive control samples (AATC) were made containing their respective E. coli DNA samples, and negative controls containing deionized water only. 9 The diagnostic targets and primers selected for this investigation are documented in the following table. 9 The samples were then transferred to a SimpliAmp TM thermal cycler (Thermo Fisher Scientific) for DNA amplification.…”
Section: Introductionmentioning
confidence: 99%
“…The following settings depicted in Table 2 were utilized. 9 The procedure was repeated for 35 cycles, with a total time of 1 hour and 22 minutes per PCR run. After DNA amplification, the samples were loaded onto an agarose gel with 2% ethidium…”
Section: Introductionmentioning
confidence: 99%
“…Briefly, the wastewater sample was inoculated into lauryl tryptose broth (Oxoid) and incubated at 35.0°C for 48 h. All tubes testing positive after the incubation period were inoculated into EC-MUG broth (Oxoid). After a 24-h incubation period at 44.5°C, tubes with a positive reaction were inoculated onto MacConkey agar plates (Oxoid) and incubated at 35°C for 24 h. The E. coli strain was identified using biochemical (API20E; BioMérieux) and molecular (16S rRNA) methods (7).…”
mentioning
confidence: 99%