Comparison of thermotolerant coliforms and Escherichia coli densities in freshwater bodies

Hachich, Elayse Maria; Bari, Marisa Di; Christ, Ana Paula Guarnieri; Lamparelli, Cláudia Condé; Ramos, Solange S.; Sato, Maria Inês Z.

doi:10.1590/s1517-83822012000200032

Cited by 48 publications

(28 citation statements)

References 13 publications

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“…Nowadays, the faecal coliforms (FC) and faecal streptococci (FS) are important common indicator microorganisms used in water quality studies. Faecal coliforms consist of more than 60% of total coliforms, and more than 90% of the faecal coliforms are members of the genus Escherichia (Hachich et al, ). It is well known that E. coli is the most prevalent coliform bacterium.…”

Section: Introductionmentioning

confidence: 99%

Measurement of antibiotic resistance of microbial species in the Karasu River (Turkey) using molecular techniques

Avşar

2018

Water & Environment J

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The Karasu River (Sinop, Turkey) was sampled for bacteriological analyses and investigation of antibiotic resistance at the bacterial isolates. In the study, the physical properties and numbers of total aerobic (TA), total coliform (TC), faecal coliform (FC), faecal Streptococci (FS) and Escherichia coli in water samples were investigated. E. coli strains were subjected to antibiotic susceptibility testing, antibiotic resistant genes, virulence genes and plasmid DNA analysis and fingerprinting techniques. The bacteriological analysis results showed that the difference between TA, TC, FC and FS numbers (p > 0.05) was not significant for a total of four stations. The Multiple Antibiotic Resistance Index (MARI) values of tested strains were found in range from 0.4 to 0.7. The antibiotic resistance genes of strains; tetA (33.9%), blacym‐2 (12.5%) and cat1 (16.7%) were screened by PCR method. Out of a total of 57 E. coli isolates, 11 E. coli strains were positive for the eae (19.6%) gene, representing atypical EPEC, whilst only one strain was positive for the eae and stx1 (1.7%) gene, representing STEC. Present study has shown that the aquatic environments can be reservoirs of pathogenic E. coli strains. The study also indicated that PCR‐based techniques might especially be helpful in the rapid identification of multidrug‐resistance analysis and virulence genes of strains. Karasu stream, which is important in Sinop province, Turkey, was investigated for the first time in this study in terms of a molecular microbiological perspective.

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Section: Introductionmentioning

confidence: 99%

Measurement of antibiotic resistance of microbial species in the Karasu River (Turkey) using molecular techniques

Avşar

2018

Water & Environment J

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“…Various external physical and chemical factors such as UV Sobsey, 2011 andNuanualsuwan et al, 2008) temperature, storage temperature and time (Soliman et al, 2013), solvents (Zaritsky et al, 1979and Rabbani et al, 2004 and pH (Maillard et al, 1994), the occurrence, viability and infectivity of coliphages (Hachich et al, 2012 andZaritsky et al, 1979). Protein coat and/or appendages besides lipid loss, and/or DNA structural changes are targets of external factors (Kamolsiripichaiporn et al, 2007 andSoliman et al, 2013).…”

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confidence: 99%

Differential Effects of Physical and Chemical Factors on Infectivity of Three Coliphages used as Water Quality Indicator

Samhan

Askora

Ezzat

et al. 2016

Egypt. J. Microbiol.

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HE EFFECT of temperature, pH, UV, petroleum ether (60-80), .......acetone (99 %), ethyl alcohol (95 %) and chloroform (99 %) on the infectivity of local coliphage isolates was studied. Three E.coli phages (ECP) were isolated from Ismailia Canal at Mostorod segment. Ability of local E.coli isolates to serve as a host was evaluated after storage at 4-6ºC, 25-28º C and 35-38º C for six months. E.coli isolate number 1 of seven tested served as host for phage reproduction and plaque formation (PF). Phage isolates were identified by plaque size and electron microscopy. ECP1 and ECP2 are Podoviridae and ECP3 is a Siphoviridae. The infectivity of coliphages decreased with increasing storage periods and incubation temperature. Exposure of somatic coliphages to acidic or alkaline pH, organic solvents or UV radiation (240 nm) or heating (≥ 60º C) diminished infectivity. Ability of E.coli1 to serve as a host for somatic coliphages inversely related to its age. No detectable effect was found for lower temperature ≤ 5º C on the infectivity of coliphage isolates during storage periods.

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“…However, although thermotolerant coliforms are associated with impacts of human activities on water quality (NIEWEGLOWSKI, 2006), they do not necessarily indicate faecal contamination. Therefore, the adoption of more accurate indicators, such as Escherichia coli, may contribute to the more effective monitoring of the water bodies (HACHICH et al, 2012). In this case, the frequency curves would be even more representative from the point of view of fecal contribution if they considered Escherichia coli.…”

Section: /13mentioning

confidence: 99%

Phosphorus and thermotolerant coliforms’ loads in Brazilian watersheds with limited data: considerations on the integrated analysis of water quality and quantity

Marques

Taffarello

Calijuri

et al. 2019

RBRH

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Integrated analyses of the qualitative and quantitative aspects of aquatic ecosystems play an important role in decision making, which is linked to water resources management and promotion of ecosystem services. However, the possible advantages of such integration are still hampered by basic aspects, such as high uncertainties due to the partial and non-synchronized monitoring of discharges and observed concentrations of water quality variables. The lack of data has been limiting the adequate representation of a more accurate hydrological regime, which is in turn possibly affected by the potential effects of land use and size of drainage areas. The aim of this research was to discuss the advantages of the integrated analysis of qualitative and quantitative data from water bodies. Under different specific discharge regimes (L.s -1.km-2), we assessed the total phosphorus (TP, t.day-1 .km-2) and thermotolerant coliforms’ (TC, CFU.day-1.km -2) specific loads in rivers with different drainage areas (27-26,500 km 2) and land uses. We compared such loads with the admissible specific loads for these variables, which were estimated based on long-term average discharges and water quality guidelines (i.e., Brazilian environmental standards). Based on data from 2001-2013, we sought to contribute to the qualitative and quantitative analysis of surface waters, focusing on the discussion of the results on critical thresholds of duration and critical loads and discharges. The results showed the existence of significant time intervals with conflicts between the specific admissible and observed loads. This non-compliance varied between 10 and 100% of the duration of the regime for TP, and between 25 and 100% for TC, with a more critical situation observed in rivers with smaller drainage areas. The critical loads ranged from 1 10-4 to 8 10-3 t.day-1.km-2 (TP) and 1 10-2 to 1 10-1 CFU.day-1.km-2 (TC). Although a relatively limited dataset was considered (total N=1,402), the significant variations in discharges and critical loads highlighted the need for considering how these dynamics would affect the traditional methodology for estimating ecological flows, whose current approach is almost exclusively quantitative.

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Comparison of thermotolerant coliforms and Escherichia coli densities in freshwater bodies

Cited by 48 publications

References 13 publications

Measurement of antibiotic resistance of microbial species in the Karasu River (Turkey) using molecular techniques

Measurement of antibiotic resistance of microbial species in the Karasu River (Turkey) using molecular techniques

Differential Effects of Physical and Chemical Factors on Infectivity of Three Coliphages used as Water Quality Indicator

Phosphorus and thermotolerant coliforms’ loads in Brazilian watersheds with limited data: considerations on the integrated analysis of water quality and quantity

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