Characterization of pyrene utilizing Bacillus spp. from crude oil contaminated soil

Khanna, Purnima; Goyal, Dinesh; Khanna, Sunil

doi:10.1590/s1517-83822012000200024

Cited by 12 publications

(6 citation statements)

References 41 publications

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“…This suggests that WS probably acted as a bioventing agent and increased oxygen penetration in the soil. Bacilli strains are known to include many oil-degrading species and were even detected in crude oils ( Ijah and Ukpe, 1992 ; Khanna et al, 2012 ; Borah and Yadav, 2014 ). An interesting feature of Bacilli strains is their ability to produce biosurfactants, which increase the bioavailability of oil through emulsification and consequently facilitate the degradation process ( Cubitto et al, 2004 ; Yamane et al, 2008 ; Perfumo et al, 2010 ; Najafi et al, 2011 ; Chandankere et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of Biostimulation Using Sewage Sludge, Soybean Meal, and Wheat Straw on Oil Degradation and Bacterial Community Composition in a Contaminated Desert Soil

Al-Kindi

Abed

2016

Front. Microbiol.

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Waste materials have a strong potential in the bioremediation of oil-contaminated sites, because of their richness in nutrients and their economical feasibility. We used sewage sludge, soybean meal, and wheat straw to biostimulate oil degradation in a heavily contaminated desert soil. While oil degradation was assessed by following the produced CO2 and by using gas chromatography–mass spectrometry (GC–MS), shifts in bacterial community composition were monitored using illumina MiSeq. The addition of sewage sludge and wheat straw to the desert soil stimulated the respiration activities to reach 3.2–3.4 times higher than in the untreated soil, whereas the addition of soybean meal resulted in an insignificant change in the produced CO2, given the high respiration activities of the soybean meal alone. GC–MS analysis revealed that the addition of sewage sludge and wheat straw resulted in 1.7–1.8 fold increase in the degraded C14 to C30 alkanes, compared to only 1.3 fold increase in the case of soybean meal addition. The degradation of ≥90% of the C14 to C30 alkanes was measured in the soils treated with sewage sludge and wheat straw. MiSeq sequencing revealed that the majority (76.5–86.4% of total sequences) of acquired sequences from the untreated soil belonged to Alphaproteobacteria, Gammaproteobacteria, and Firmicutes. Multivariate analysis of operational taxonomic units placed the bacterial communities of the soils after the treatments in separate clusters (ANOSIM R = 0.66, P = 0.0001). The most remarkable shift in bacterial communities was in the wheat straw treatment, where 95–98% of the total sequences were affiliated to Bacilli. We conclude that sewage sludge and wheat straw are useful biostimulating agents for the cleanup of oil-contaminated desert soils.

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Section: Discussionmentioning

confidence: 99%

Effect of Biostimulation Using Sewage Sludge, Soybean Meal, and Wheat Straw on Oil Degradation and Bacterial Community Composition in a Contaminated Desert Soil

Al-Kindi

Abed

2016

Front. Microbiol.

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“…Few studies have been conducted and published to date on pyrene degradation by bacterial cultures, particularly Bacillus sp. Earlier, Khanna et al reported Bacillus sp. PK‐12, Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…However, no metabolites were detected in earlier studies of pyrene degradation using Bacillus sp. . In separate studies, Bacillus species have been shown to degrade pyrene with toxic/dead‐end by‐products .…”

Section: Discussionmentioning

confidence: 99%

“…Owing to wide ecological amplitude and prevalence in environment, Bacillus has been one of the favorites of ecologists to remediate various abiotic and biotic stresses. However, limited information is available on its metabolic pathway of pyrene degradation . Earlier attempts to isolate Bacillus species which can degrade pyrene yielded toxic and dead‐end metabolites .…”

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of Bacillus megaterium YB3 from an effluent contaminated site efficiently degrades pyrene

Meena

Sharma

Gupta³

et al. 2016

J. Basic Microbiol.

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Industrial effluents contaminated sites may serve as repositories of ecologically adapted efficient pyrene degrading bacteria. In the present study, six bacterial isolates from industrial effluents were purified using serial enrichment technique and their pyrene degrading potential on pyrene supplemented mineral salt medium was assessed. 16S rRNA sequence analysis showed that they belong to four bacterial genera, namely Acinetobacter, Bacillus, Microbacterium, and Ochrobactrum. Among these isolates, Bacillus megaterium YB3 showed considerably good growth and was further evaluated for its pyrene-degrading efficiency. B. megaterium YB3 could degrade 72.44% of 500 mg L(-1) pyrene within 7 days. GC-MS analysis of ethyl acetate extracted fractions detected two relatively less toxic metabolic intermediates of the pyrene degradation pathway. B. megaterium YB3 also tested positive for catechol 1, 2-dioxygenase and aromatic-ring-hydroxylating dioxygenase indole-indigo conversion assays. Considering the ability and efficiency of B. megaterium YB3 to degrade high pyrene content, the strain can be used as a tool to develop bioremediation technologies for the effective biodegradation of pyrene and possibly other PAHs in the environment.

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“…Previous investigations have reported that co-metabolism triggered by glucose could significantly accelerate the degradation of PAHs under both aerobic and anaerobic digestion environments ( Ambrosoli et al, 2005 ; Ma et al, 2011 ; Qin et al, 2018 ; Tripathi et al, 2020 ; Al Farraj et al, 2021 ). For example, the addition of glucose was reported to significantly enhance the degradation of PAHs (benzopyrene, anthracene, and pyrene) when treated with some pure bacterial species, such as Cellulosimicrobium , Sphingomonas , and Bacillus ( Khanna et al, 2012 ; Qin et al, 2018 ; Al Farraj et al, 2021 ). Additionally, PAH degradation under methanogenesis is thermodynamically feasible and the degradation of PAHs can be coupled with methanogenesis, suggesting that remediation of PAHs pollution via the anaerobic digestion system should be a potentially ideal method marked by the properties of energy conservation and resource reuse ( Chang et al, 2005 ; Dolfing et al, 2009 ; Berdugo-Clavijo et al, 2012 ; Wan et al, 2012 ; Nzila, 2018 ; Ye et al, 2019 ; Wang et al, 2021b ).…”

Section: Introductionmentioning

confidence: 99%

Co-metabolic Effect of Glucose on Methane Production and Phenanthrene Removal in an Enriched Phenanthrene-Degrading Consortium Under Methanogenesis

Zhou

Wang

et al. 2021

Front. Microbiol.

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Anaerobic digestion is used to treat diverse waste classes, and polycyclic aromatic hydrocarbons (PAHs) are a class of refractory compounds that common in wastes treated using anaerobic digestion. In this study, a microbial consortium with the ability to degrade phenanthrene under methanogenesis was enriched from paddy soil to investigate the cometabolic effect of glucose on methane (CH4) production and phenanthrene (a representative PAH) degradation under methanogenic conditions. The addition of glucose enhanced the CH4 production rate (from 0.37 to 2.25mg⋅L−1⋅d−1) but had no influence on the degradation rate of phenanthrene. Moreover, glucose addition significantly decreased the microbial α-diversity (from 2.59 to 1.30) of the enriched consortium but showed no significant effect on the microbial community (R2=0.39, p=0.10), archaeal community (R2=0.48, p=0.10), or functional profile (R2=0.48, p=0.10). The relative abundance of genes involved in the degradation of aromatic compounds showed a decreasing tendency with the addition of glucose, whereas that of genes related to CH4 synthesis was not affected. Additionally, the abundance of genes related to the acetate pathway was the highest among the four types of CH4 synthesis pathways detected in the enriched consortium, which averagely accounted for 48.24% of the total CH4 synthesis pathway, indicating that the acetate pathway is dominant in this phenanthrene-degrading system during methanogenesis. Our results reveal that achieving an ideal effect is diffcult via co-metabolism in a single-stage digestion system of PAH under methanogenesis; thus, other anaerobic systems with higher PAH removal efficiency should be combined with methanogenic digestion, assembling a multistage pattern to enhance the PAH removal rate and CH4 production in anaerobic digestion.

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Characterization of pyrene utilizing Bacillus spp. from crude oil contaminated soil

Cited by 12 publications

References 41 publications

Effect of Biostimulation Using Sewage Sludge, Soybean Meal, and Wheat Straw on Oil Degradation and Bacterial Community Composition in a Contaminated Desert Soil

Effect of Biostimulation Using Sewage Sludge, Soybean Meal, and Wheat Straw on Oil Degradation and Bacterial Community Composition in a Contaminated Desert Soil

Isolation and identification of Bacillus megaterium YB3 from an effluent contaminated site efficiently degrades pyrene

Co-metabolic Effect of Glucose on Methane Production and Phenanthrene Removal in an Enriched Phenanthrene-Degrading Consortium Under Methanogenesis

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