Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

Santos, Carla Rocha dos; Franciscatto, Laura G.; Barcellos, Regina Bones; Almeida, Sabrina Esteves de Matos; Rossetti, Maria Lúcia Rosa

doi:10.1590/s1517-83822012000100047

Cited by 8 publications

(5 citation statements)

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“…Due to the logistical difficulties such as spillage, flammability, and weight, adding to the risks and costs of liquid transport media, solid carriers consisting of chemically treated or untreated filter paper have been investigated for hrHPV testing. These filter paper cards are easy and safe to store and transport [1][2][3][4][5][6][7][8][9][10][11][12][13]. Recently we (Maurer, et.al.…”

Section: Introductionmentioning

confidence: 99%

An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens

Duan

Yan

et al. 2020

Infect Agents Cancer

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Background The solid transport media is a small size card that allows fast, easy DNA extraction from a variety of biological samples. In 2016 we developed a solid media transport card; for that pilot study to control the self-collection we used a pseudo-self-collection technique. The current study expands this prior work using true self-collections and only the POI card, and aims to evaluate the solid media transport card to detect HR-HPV in self-samples compared to liquid transport media. Methods Ten thousand eight hundred eighty-five women between the ages of 30–59 with no screening for 3 years were enrolled. The self-collected sample was first applied to a new solid media transport card (Labeled as SC) then the brush placed in 6 ml ThinPrep liquid (Labeled as SL). Then a physician collected a direct endocervical specimen into ThinPrep liquid (Labeled as DL). Samples were tested with Cobas 4800 and the SeqHPV NGS assay for HR-HPV. Patients positive on any test were recalled for colposcopy and biopsy. Results Ten thousand three hundred thirty-nine participants had complete data. The mean age was 43.9 years. CIN 2+ rates were 1.4% (142/10339). The agreement in HPV detection between the two different self-sample collection media was also good (Cobas HPV kappa = 0.86; SeqHPV kappa = 0.98). Tested with Cobas, the sensitivity of Cobas-SL and Cobas-SC for CIN 2+ was95.07 and 94.37%; and for CIN3+ was 96.30, 96.30% respectively. The specificity of Cobas-SC, and Cobas-SL for CIN2+ was 88.74 and 87.35%; for CIN3 was 88.04and 86.65% respectively. Tested with SeqHPV, the sensitivity for CIN2+ of Seq-SC and Seq-SL was 95.77 and 96.48%; for CIN3+, both the SC and SL specimens had a sensitivity of 100%. The specificity for CIN2+ of Seq-SC and Seq-SL was 89.54 and 89.53%; for CIN3+ was 88.84,88.82% respectively. For both HR-HPV assays, the sensitivities were similar for the two self-sample media (SC vs SL, p = 1.00). Conclusions The solid transport card for collecting vaginal self-samples as accurate as liquid transport media assayed by two different PCR based HR-HPV tests. The solid transport media is a suitable medium for collecting and storing vaginal self-samples.

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Section: Introductionmentioning

confidence: 99%

An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens

Duan

Yan

et al. 2020

Infect Agents Cancer

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“…In terms of the card specifically, there has been a series of pilot studies demonstrating that the card, like any self-collection medium, has very good agreement with the clinician-collected sample [3–10]. In a recently published study, we demonstrated that there was good 90% total agreement, 68% positive agreement and κ-value of 0.75 between a standard specimen medium and paper/card-based medium for HR-HPV detection [11].…”

Section: How Do Self-collection Methods Compare With Traditional Scrementioning

confidence: 99%

Current Status and Future Trends in Cervical Cancer Screening

Castle¹

2014

Womens Health (Lond Engl)

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Dr Castle has served as an invited speaker or session chair in many forums, including for the American Association for Cancer Research, International Papillomavirus Society and the European Union on Genital Infection and Neoplasia (EUROGIN). He regularly participates in the development of national and international guidelines for cervical cancer prevention. Dr Castle serves as a consultant for several countries on the development of national cervical cancer prevention programs, including pilot/demonstration projects in El Salvador and Vietnam. He is currently a member of the CDC National Breast and Cervical Cancer Early Detection and Prevention Advisory Committee. For his work in cervical cancer prevention, Dr Castle has received an EUROGIN Distinguished Service Award (2006), a NIH Merit Award for introduction of HPV testing into low-resource settings in the USA

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“…For this reason, high sensitivity primers should be chosen to perform PCRs with FP. Santos (2012) shows that the direct use of the FTA TM elute card in the mix was more effective in identifying HPV-DNA (92% of positive samples) than DNA extracted from the card by elution (54%) 11 . Studies involving Leishmania that used FP for the collection of lesion scrapings, biopsy, or lesion imprint had greater sensitivity (92% to 100%) compared to DS or PCR with DNA obtained from lesion aspirate or biopsy.…”

Section: P C R W a S P E R F O R M E D U S I N G T H E P R I M E R S mentioning

confidence: 99%

“…FP does not appear to interfere with DNA amplification. The increase in temperature of the PCR mix was probably enough for the DNA to escape the FP, thus allowing an efficient reaction 11 .…”

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Filter paper performance in PCR for cutaneous leishmaniasis diagnosis

Mota

Venazzi

Zanzarini

et al. 2021

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis. METHODS: Lesion scrapings from 48 patients were collected and analyzed for PCR. RESULTS: PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%. CONCLUSIONS: FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR.

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Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

Cited by 8 publications

References 0 publications

An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens

An evaluation of solid versus liquid transport media for high-risk HPV detection and cervical cancer screening on self-collected specimens

Current Status and Future Trends in Cervical Cancer Screening

Filter paper performance in PCR for cutaneous leishmaniasis diagnosis

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