Molecular characterisation and biomass and metabolite production of Lactobacillus reuteri LPB P01-001: a potential probiotic

Pancheniak, Elizete de Fátima Reque; Maziero, Maike Taís; Rodríguez-León, José A.; Parada, José; Spier, Michele Rigon; Soccol, Carlos Ricardo

doi:10.1590/s1517-83822012000100015

Cited by 13 publications

(7 citation statements)

References 28 publications

(32 reference statements)

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“…In this study, firstly, the DhBCS004 growth curve and biomass production were evaluated. Pancheniak et al (2012) obtained a production of 2.0 g/L biomass of Lactobacillus reuteri LPB P01‐001 after 28 h of fermentation under pH control. Based on the result in this study, DhBCS004 had both properties, the necessary fast growth and high‐cell density to recommend commercial production of probiotic biomass.…”

Section: Discussionmentioning

confidence: 99%

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Probiotic properties of Debaryomyces hansenii BCS004 and their immunostimulatory effect in supplemented diets for gilthead seabream ( Sparus aurata )

Reyes‐Becerril

Angulo

et al. 2021

Aquaculture Research

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Debaryomyces hansenii BCS004 strain is a non‐pathogenic, polyamine and superoxide dismutase secreting marine yeast. The objective of this study was to investigate the marine yeast DhBCS004 as probiotics and evaluate its antimicrobial properties and dietary immunostimulant effect on gilthead seabream specimens when administered at 106 colony‐forming unit/g−1 or 1.1% for 4 weeks. This yeast strain showed potential probiotic properties, including stability in gastrointestinal survival (pH, bile conditions) and attachment to intestinal cells. The DhBCS004 had antimicrobial activity against important marine pathogens. Interestingly, this yeast has a high amount of n−3 polyunsaturated fatty acids with high concentrations of oleic and linoleic acids, which may confer nutritional and therapeutic values. The in vivo immunological assay revealed that dietary DhBCS004 significantly increased phagocytosis capacity, peroxidase and respiratory burst activities in leucocytes. Furthermore, the natural haemolytic complement activity and total IgM levels increased in serum after weeks 2 or 4 of trial. Finally, DhBCS004 significantly increased the transcriptions of TNF‐α, C3 and IgM in intestine samples. This work reinforces the biotechnological and immunological potential of D. hansenii BCS004 in the field of fish aquaculture.

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Section: Discussionmentioning

confidence: 99%

“…were evaluated Pancheniak et al (2012). obtained a production of 2.0 g/L biomass of Lactobacillus reuteri LPB P01-001 after 28 h of fermentation under pH control.…”

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confidence: 99%

Probiotic properties of Debaryomyces hansenii BCS004 and their immunostimulatory effect in supplemented diets for gilthead seabream ( Sparus aurata )

Reyes‐Becerril

Angulo

et al. 2021

Aquaculture Research

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“…In one study, the optimal temperature for the production of Lactobacillus plantarum biomass was observed in the range 34–39.6°C (Manzoor et al, 2017). Pancheniak et al (2012) used a simple culture medium based on cane sugar and yeast extract in the production of Lactobacillus reuteri LPB P01–001 biomass. They observed that the biomass concentration increased during 28 h of fermentation at 37°C.…”

Section: Resultsmentioning

confidence: 99%

Optimization of fermentation process of date syrup by Lactobacillus delbrueckii and Lactobacillus acidophilus : Microbial growth, carbohydrate metabolism, and peptide content

Gholamhosseinpour

Hashemi

2022

Food Processing Preservation

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Response surface methodology was employed to investigate the combined effects of syrup concentration (10%, 20%, 30%), incubation temperature (35, 37, 39°C), fermentation time (16, 20, 24 h), and strain type (Lactobacillus delbrueckii and Lactobacillus acidophilus) on glucose and fructose content, biomass concentration and peptide content of fermented date syrup as responses. The fitted second‐order polynomial models were highly adequate to predict the responses. Fermentation temperature had a significant effect on all responses, while none of them were significantly affected by the strain type. The estimated optimal conditions of fermented date syrup for the highest biomass and peptide content were 16 h of fermentation time, 38.43°C of incubation temperature, 19.92% of syrup concentration and L. acidophilus as a starter culture. Consequently, the quadratic model was validated by applying the estimated optimum conditions, which confirmed the model validity where 4.55 g/L glucose, 2.59 g/L fructose, 4.83 biomass, and 10.30 g/L of peptide were produced. Novelty impact statement The high content of sugar, high amounts of fiber, the presence of natural antioxidants, and having functional properties, justify the industrial use of date syrup as a suitable fermentation substrate for the production of fermented food products. No study has yet been conducted to optimize the fermentation conditions (temperature and time of fermentation and sugar concentration) of date syrup. In this research, we optimized the mentioned conditions using response surface methodology to maximize lactic acid bacteria growth and increase final product functionality.

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“…Conversely, the growth rate of C. albicans was computed at 0.236 h −1 when alone and 0.419 h −1 when in presence of S. mutans . The L. reuteri growth rate was derived from the public domain: 0.177 h −1 [19] and was considered constant. The model considered an initial seed of 1 × 10 4 CFU/mL for both S. mutans and C. albicans , and 1 × 10 8 CFU/mL for L. reuteri .…”

Section: Resultsmentioning

confidence: 99%

Identification of viral dose and administration time in simulated phage therapy occurrences

Plunder

Helling

Venturelli

et al. 2022

Preprint

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The rise in multidrug-resistant bacteria has sprung a renewed interest in applying phages as antibacterial, a procedure Western practitioners eventually abandoned due to several downfalls, including poor understanding of the dynamics between phages and bacteria. A successful phage therapy needs to account for the loss of infective virions and the multiplication of the hosts. The parameters critical inoculation size (VF) and failure threshold time (TF) have been introduced to assure that the viral dose (vΦ) and administration time (tΦ) would lead to an effective treatment. The problem with the definition of VF and TF is that they are non-linear equations with two unknowns; thus, their solution is cumbersome and not unique. The current study used machine learning in the form of a decision tree algorithm to determine ranges for the viral dose and administration times required to achieve an effective phage therapy. Within these ranges, a Pareto optimal solution of a multi-criterial optimization problem (MCOP) provides values leading to effective treatment. The algorithm was tested on a series of microbial consortia that described allochthonous invasions (the outgrowing of a species at high cell density by another species initially present at low concentration) to inhibit the growth of the invading species. The present study also introduced the concept of 'mediated phage therapy', where targeting a booster bacteria might decrease the virulence of a pathogen immune to phagial infection. The results demonstrated that the MCOP could provide pairs of vΦ and tΦ that could effectively wipe out the bacterial target from the considered micro-environment. In summary, the present work introduced a novel method for investigating the phage/bacteria interaction that could help increase the effectiveness of phage therapy.

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Molecular characterisation and biomass and metabolite production of Lactobacillus reuteri LPB P01-001: a potential probiotic

Cited by 13 publications

References 28 publications

Probiotic properties of Debaryomyces hansenii BCS004 and their immunostimulatory effect in supplemented diets for gilthead seabream ( Sparus aurata )

Probiotic properties of Debaryomyces hansenii BCS004 and their immunostimulatory effect in supplemented diets for gilthead seabream ( Sparus aurata )

Optimization of fermentation process of date syrup by Lactobacillus delbrueckii and Lactobacillus acidophilus : Microbial growth, carbohydrate metabolism, and peptide content

Identification of viral dose and administration time in simulated phage therapy occurrences

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