Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract

Jain, Sumat Chand; Jain, P. C.; Kango, Naveen

doi:10.1590/s1517-83822012000100007

Cited by 30 publications

(11 citation statements)

References 23 publications

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“…The genus Kluyveromyces is utilized as a source of different enzymes, among which β-galactosidase (β-D-galactohydrolase, EC 3.2.1.23) (β-gal) (Barreto et al , 2000; Dagbagli and Goksungur, 2008) and inulinase (Jain et al , 2012). In particular, the species Kluyveromyces lactis can use lactose as the only carbon and energy source because of a high β-gal activity.…”

Section: Introductionmentioning

confidence: 99%

Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology

Faria¹,

Rocha²,

Converti³

et al. 2013

Braz. J. Microbiol.

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The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under different conditions of ethanol concentration, treatment time and temperature according to a central composite rotatable design (CCRD), and the collected results were then worked out by response surface methodology (RSM). Cell permeabilization was improved by an increase in ethanol concentration and simultaneous decreases in the incubation temperature and treatment time. Such an approach allowed us to identify an optimal range of the independent variables within which the β-galactosidase activity was optimized. A maximum permeabilization of 2,816 mmol L−1 oNP min−1 g−1 was obtained by treating cells with 75.0% v/v of ethanol at 20.0 °C for 15.0 min. The proposed methodology resulted to be effective and suited for K. lactis cells permeabilization at a lab-scale and promises to be of possible interest for future applications mainly in the food industry.

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Section: Introductionmentioning

confidence: 99%

Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology

Faria¹,

Rocha²,

Converti³

et al. 2013

Braz. J. Microbiol.

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“…Other authors have also reported similar optimum pH and temperature values for K. marxianus [38,39,46,49], but inulinases from other fungi species have also presented similar biochemical characteristics [17,26,50]. This characteristic is very interesting for the industrial sector, because acid pH and higher temperatures decrease the risk of contamination [16].…”

Section: Discussionmentioning

confidence: 67%

“…Interest has grown in inulin, a polyfructans widespread among plants as source of reserve energy, because it is a relatively cheap and abundant substrate for fructose syrup and ethanol [8]. The plant substrates that contain inulin most described in the literature as culture medium for the fermentation processes are Jerusalem artichoke [6,21,37], chicory root [22,26] and dahlia tubers [38]. Yacon has only recently been studied as a fermentation substrate (39).…”

Section: Discussionmentioning

confidence: 99%

Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571

Sathler

Contiero

Cazetta

2014

JBT

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Yacon is a perennial plant native of the Andes that stores inulin-type polyfructans as reserve carbohydrates in its tubers. The enzymes that hydrolyze the inulin β-2,1 fructose linkages are called inulinases and are much used to produce high fructose syrups (HFS) and ethanol. The present study investigated inulinase and biomass production by Kluyveromyces marxianus NRRL Y-7571 using yacon flour as carbon source and corn steep liquor as nitrogen source by a Central Composite Design (CCD) 2 2 to carry out experiments and Surface Response Methodology (RSM) to analyze the results. The maximum enzyme production was 36.1 U.ml -1 and biomass production was 8.95 g.l -1 under the condition of 7% yacon flour and 2% corn steep liquor. The crude enzyme partial characterization showed that inulinase had optimal pH at 4.0 and optimal temperature at 60°C. The enzyme maintained over 90% of its activity for four hours at 45°C and 50°C.

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“…4 a, b). As the INU1 product inulinase metabolizes inuline to fructose (Rouwenhorst et al 1988 ), INU1 is downregulated in the presence of glucose, the preferred sugar (Jain et al 2012 ). Moreover, INU1 gene expression is known to be up-regulated when fructose replaces glucose as a sugar source (Schabort et al 2016 ), and the INU1 promoter has consensus binding sequences for MIG1, a known repressor of transcription in the presence of glucose (Bergkamp et al 1993 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic and physiological basis for antibody production by Kluyveromyces marxianus

et al. 2018

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Kluyveromyces marxianus is a thermotolerant, crabtree-negative yeast, which preferentially directs metabolism (e.g., from the tricarboxylic acid cycle) to aerobic alcoholic fermentation. Thus K. marxianus has great potential for engineering to produce various materials under aerobic cultivation conditions. In this study, we engineered K. marxianus to produce and secrete a single-chain antibody (scFv), a product that is highly valuable but has historically proven difficult to generate at large scale. scFv production was obtained with strains carrying either plasmid-borne or genomically integrated constructs using various combinations of promoters (PMDH1 or PACO1) and secretion signal peptides (KmINUss or Scα-MFss). As the wild-type K. marxianus secretes endogenous inulinase predominantly, the corresponding INU1 gene was disrupted using a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)—associated protein (CRISPR–Cas9) system to re-direct resources to scFv production. Genomic integration was used to replace INU1 with sequences encoding a fusion of the INU1 signal peptide to scFv; the resulting construct yielded the highest scFv production among the strains tested. Optimization of growth conditions revealed that scFv production by this strain was enhanced by incubation at 30 °C in xylose medium containing 200 mM MgSO4. These results together demonstrate that K. marxianus has the potential to serve as a host strain for antibody production.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0588-1) contains supplementary material, which is available to authorized users.

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Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract

Cited by 30 publications

References 23 publications

Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology

Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology

Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571

Genetic and physiological basis for antibody production by Kluyveromyces marxianus

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