Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity

Gong, Tianxiang; Li, Wanyi; Wang, Yueling; Jiang, Yan; Zhang, Qiang; Feng, Wei; Jiang, Zhonghua; Li, Mingyuan

doi:10.1590/s1517-83822011000300043

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…Mucin 2 is the main component of the mucus layer that keeps intestinal bacteria at a distance from the intestinal epithelium. The inner mucus layer contains various antimicrobial peptides produced by epithelial cells that exhibit bactericidal effects (70, 71). Colonization with SFB was shown to ameliorate colitis induced by the intestinal pathogen Citrobacter rodentium by increased expression of pro-inflammatory cytokines and antimicrobial defenses (31).…”

Section: Discussionmentioning

confidence: 99%

Composition of the Intestinal Microbiota Determines the Outcome of Virus-Triggered Colitis in Mice

et al. 2019

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The intestinal microbiota is a complex ecosystem implicated in host health and disease. Inflammatory bowel disease (IBD) is a multifactorial chronic disorder of the gastrointestinal mucosa. Even though the exact mechanisms are still unknown, the intestinal microbiota is crucial in IBD development. We previously showed that murine norovirus (MNV) induces colitis in the Il10 -deficient ( Il10 −/− ) mouse model of IBD in a microbiota-dependent manner. Thus, in this study we analyzed whether distinct minimal bacterial consortia influence the outcome of MNV-triggered colitis in Il10 −/− mice. Gnotobiotic Il10 −/− mice associated with Oligo-Mouse-Microbiota 12 (OMM 12 ) or Altered Schaedler Flora (ASF) developed little to no inflammatory lesions in the colon and cecum. MNV infection exacerbated colitis severity only in ASF-colonized mice, but not in those associated with OMM 12 . Four weeks after MNV infection, inflammatory lesions in ASF-colonized Il10 −/− mice were characterized by epithelial hyperplasia, infiltration of inflammatory cells, and increased barrier permeability. Co-colonization of ASF-colonized Il10 −/− mice with segmented filamentous bacteria (SFB) abolished MNV-induced colitis, whereas histopathological scores in SFB-OMM 12 -co-colonized mice stayed unchanged. Moreover, SFB only colonized mice associated with ASF. The SFB-mediated protective effects in ASF-colonized mice involved enhanced activation of intestinal barrier defense mechanisms and mucosal immune responses in the chronic and acute phase of MNV infection. SFB colonization strengthened intestinal barrier function by increasing expression of tight junction proteins, antimicrobial peptides and mucus. Furthermore, SFB colonization enhanced the expression of pro-inflammatory cytokines such as Tnf α, Il1 β, and Il12a , as well as the expression of the regulatory cytokine Tgf β. Altogether, our results showed that MNV-triggered colitis depends on the microbial context.

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Section: Discussionmentioning

confidence: 99%

Composition of the Intestinal Microbiota Determines the Outcome of Virus-Triggered Colitis in Mice

et al. 2019

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Yak DEFB124 alleviates intestinal injury caused by Staphylococcus aureus infection

Zhang

Mei

Wang

et al. 2023

International Immunopharmacology

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Interaction of antimicrobial peptide Plantaricin149a and four analogs with lipid bilayers and bacterial membranes

Lopes

Hissa

Melo

et al. 2013

Braz. J. Microbiol.

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The amidated analog of Plantaricin149, an antimicrobial peptide from Lactobacillus plantarum NRIC 149, directly interacts with negatively charged liposomes and bacterial membranes, leading to their lysis. In this study, four Pln149-analogs were synthesized with different hydrophobic groups at their N-terminus with the goal of evaluating the effect of the modifications at this region in the peptide’s antimicrobial properties. The interaction of these peptides with membrane models, surface activity, their hemolytic effect on red blood cells, and antibacterial activity against microorganisms were evaluated. The analogs presented similar action of Plantaricin149a; three of them with no hemolytic effect (< 5%) until 0.5 mM, in addition to the induction of a helical element when binding to negative liposomes. The N-terminus difference between the analogs and Plantaricin149a retained the antibacterial effect on S. aureus and P. aeruginosa for all peptides (MIC50 of 19 μM and 155 μM to Plantaricin149a, respectively) but resulted in a different mechanism of action against the microorganisms, that was bactericidal for Plantaricin149a and bacteriostatic for the analogs. This difference was confirmed by a reduction in leakage action for the analogs. The lytic activity of Plantaricin149a is suggested to be a result of the peptide-lipid interactions from the amphipathic helix and the hydrophobic residues at the N-terminus of the antimicrobial peptide.

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Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity

Cited by 3 publications

References 13 publications

Composition of the Intestinal Microbiota Determines the Outcome of Virus-Triggered Colitis in Mice

Composition of the Intestinal Microbiota Determines the Outcome of Virus-Triggered Colitis in Mice

Yak DEFB124 alleviates intestinal injury caused by Staphylococcus aureus infection

Interaction of antimicrobial peptide Plantaricin149a and four analogs with lipid bilayers and bacterial membranes

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