Evaluation of sorghum straw hemicellulosic hydrolysate for biotechnological production of xylitol by Candida guilliermondii

Sene, Luciane; Arruda, Priscila Vaz de; Oliveira, S.M.M; Felipe, M.G.A

doi:10.1590/s1517-83822011000300036

Cited by 29 publications

(16 citation statements)

References 3 publications

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“…Other xylitol fermentation research utilizing Candida guilliermondii cultivated on hemicellulosic hydrolysates from forage sorghum generated a xylitol yield around 0.35 g xylitol/g xylose consumed which also compares well with the yield calculated from Figure 4 [35]. Using C. guilliermondii on sorghum straw hydrolysate produced a yield of 0.44 g xylitol/g xylose consumed, which slightly outperforms the yield obtained here [36]. The results indicated that although the stillage media could support xylitol production, it might contain inhibitory compounds, which negatively affect the consumption rates of the two substrates, i.e., xylose and glycerol, especially when they accumulated to high levels due to concentration.…”

Section: Stillage Media With Detoxificationsupporting

confidence: 77%

Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production

2019

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Developing integrated biorefineries requires the generation of high-value co-products produced alongside cellulosic ethanol. Most industrial yeast strains produce ethanol at high titers, but the small profit margins for generating ethanol require that additional high-value chemicals be generated to improve revenue. The aim of this research was to boost xylose utilization and conversion to high-value co-products that can be generated in an integrated biorefinery. Pretreated sweet sorghum bagasse (SSB) was hydrolyzed in sweet sorghum juice (SSJ) followed by ethanol fermentation. Ethanol was removed from the fermentation broth by evaporation to generate a stillage media enriched in xylose. Candida mogii NRRL Y-17032 could easily grow in non-detoxified stillage media, but a high xylitol yield of 0.55 g xylitol/g xylose consumed was achieved after recovered cells were resuspended in synthetic media containing supplemented xylose. Phaffia rhodozyma ATCC 74219 could be cultivated in non-detoxified stillage media, but astaxanthin generation was increased 4-fold (from 17.5 to 71.7 mg/L) in detoxified media. Future processing strategies to boost product output should focus on a two-step process where the stillage media is used as the growth stage, and a synthetic media for the production stage utilizing xylose generated from SSB through selective hemicellulase enzymes.

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Section: Stillage Media With Detoxificationsupporting

confidence: 77%

Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production

2019

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“…The inhibitors concentrations (acetic acid 3.56 g L -1 , furfural 0.03 g L -1 , HMF 0.03 g L -1 and total phenols 0.89 g L -1 ) are within or under the range of values usually found in other sources of hemicellulosic hydrolysates, in general, 1-3.41 g L -1 of acetic acid, 0.04-0.26 g L -1 of furfural, 0.008-1.56 g L -1 of HMF and 2.12-2.23 g L -1 of total phenols (Mussatto & Roberto, 2004;Canilha et al, 2005;Marton et al, 2006;Villarreal et al, 2006;Sene et al, 2011).…”

Section: Methodssupporting

confidence: 75%

Valorization of sunflower meal through the production of ethanol from the hemicellulosic fraction

Tavares

Sene

Christ

2016

Rev. bras. eng. agríc. ambient.

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A B S T R A C TSunflower is among the major oil seeds crop grown in the world and the by-products generated during the seeds processing represent an attractive source of lignocellulosic biomass for bioprocesses. The conversion of lignocellulosic fibers into fermentable sugars has been considered as a promising alternative to increase the demand for ethanol. The present study aimed to establish the fermentation conditions for ethanol production by Scheffersomyces stipitis ATCC 58376 in sunflower meal hemicellulosic hydrolysate, through a 2 3 CCRD (Central Composite Rotational Design) factorial design. Under the selected conditions (pH 5.25, 29 ºC and 198 rpm) the final ethanol concentration was 13.92 g L -1 and the ethanol yield was 0.49 g g -1 .Valorização do farelo de girassol através da produção de etanol a partir da fração hemicelulósica R E S U M O O girassol está entre as principais oleaginosas cultivadas no mundo e os subprodutos gerados durante o processamento das sementes representam uma fonte atraente de biomassa lignocelulósica para bioprocessos. A conversão de fibras lignocelulósicas em açúcares fermentáveis tem sido considerada uma alternativa promissora para aumentar a demanda de etanol. O presente estudo teve, como objetivo, estabelecer as condições de fermentação para a produção de etanol por Scheffersomyces stipitis ATCC 58376 em hidrolisado hemicelulósico de farelo de girassol através de um planejamento fatorial DCCR 2 3 . Sob as condições selecionadas (pH 5,25, 29 ºC e 198 rpm) a concentração final de etanol foi 13,92 g L -1 e o rendimento 0,49 g g -1 .

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“…The quadratic trend can be related to an increased presence of inhibitor compounds, such as phenols (41,42) , that are released in higher extension as the severity of STE is greater. The total content of phenols compounds reported is 0.28 (43) , 1.35 (44) and 0.52 (45) g/100 g for COS, SOS and SUB, respectively. Another possibility is that as hemicellulose is solubilized, the proportion of crystalline cellulose increases, resulting in lower digestibility.…”

Section: Resultados Y Discusiónmentioning

confidence: 93%

“…La tendencia cuadrática puede estar relacionada a una mayor presencia de compuestos inhibidores, tales como compuestos fenólicos (41,42) que se incrementan según aumenta la severidad de la EXPV. El contenido total de compuestos fenólicos reportados son 0.28 (43) , 1.35 (44) y 0.52 (45) g/100 g para RM, RS y BZC. Otra posibilidad es que en el material tratado al solubilizarse la hemicelulosa, se incrementa la proporción de celulosa cristalina que se digiere más lentamente.…”

Section: Kinetics Of In Situ Digestionunclassified

Composición química, digestibilidad y cinética ruminal de la digestión de residuos agrícolas tratados con explosión de vapor

Gutiérrez

Martínez²,

Vega

et al. 2018

Rev. Mex. Cienc. Pecu.

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RESUMENPara determinar el efecto de la explosión de vapor (EXPV) en la composición y digestibilidad de forrajes toscos (F) se condujeron dos experimentos. En el Exp 1, con un diseño factorial de presión (P; 10.3, 13.8 y 17.2 kg cm 2 ) y tiempo (T; 5, 10 y 15 min), se determinaron los cambios en pH, fracciones de fibra (FF) y la digestibilidad in vitro (DIVMS) del rastrojo de sorgo (RS), rastrojo de maíz (RM) y bagazo de caña (BZC) por efecto de EXPV. En el Exp 2, se comparó la cinética de la digestión in situ (CDI) de forrajes sin tratar y tratados con EXPV, RS y RM fueron tratados con 13.8 kg/cm 2 por 10 min y BZC con 17.2 kg/cm 2 por 15 min. Se incubaron muestras de forraje en rumen hasta por 120 h. Los datos fueron ajustados a un modelo no lineal. P y T no interactuaron en pH o FF. El aumento en P o T redujeron el pH, FDN y la hemicelulosa. La lignina y FDA no cambiaron dentro los niveles de P o T. En DIVMS, P*F y T*F fueron significativas. EXPV incrementó las fracciones soluble y potencialmente digestible y extensión de digestión de FF de los tres forrajes. Exceptuando para BCZ, el tiempo de retraso no cambió para FF. La respuesta de la tasa de digestión fue variable para FF o forraje. Se concluye que la EXPV mejora la calidad nutritiva de los forrajes toscos por solubilizar parte de la pared celular, acelerar la tasa de digestión e incrementar la extensión de la digestión. PALABRAS CLAVE: Explosión de vapor, Forrajes toscos, Fibra, Digestibilidad, Cinética digestiva. ABSTRACTTwo experiments were conducted to determine the effects of steam explosion (STE) on chemical composition and digestibility of the following forages (F): sorghum stover (SOS), corn stover (COS) and sugarcane bagasse (SUB). The Exp 1 was conducted to determine the effects of STE on pH, fiber fractions (FF) and in vitro digestibility (IVDMD). Fifty four samples were generated using a RCB design with a 3*3*3 factorial treatment arrangement, where the factors were: pressure (P: 10.3, 13.8 and 17.2 kg/cm 2 ), time (T: 5, 10 and 15 min) and F (SOS, COS and SUB). In Exp 2, changes in the digestion kinetics were determined when using the combinations of P and T that maximized IVDMD; thus, SOS and COS were treated at 13.8 kg/cm 2 for 10 min, and SUB at 17.2 kg/ cm 2 for 15 min. The treated and non-treated samples were incubated in rumen for up to 120 h. Data were fitted to a nonlinear model. No significant interactions T*P or F*P*T were detected on pH or FF; however, as P or T increased, pH, NDF and hemicellulose decreased. Lignin or ADF were not affected by P or T. As for IVDMD concerns, P*F and F*T interactions were significant. STE increased soluble fraction and degree of F digestion. Only lag time of SUB was reduced by STE. The potential digestible fraction was reduced in SOS and COS. It was concluded that the changes in chemical composition and digestion kinetics are related mainly to cell wall solubilization by stem explosion.KEY WORDS: Steam-explosion, Crop residues, Fiber, Digestibility, Digestion kinetics. INTRODUCCIÓNCon base en la sup...

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Evaluation of sorghum straw hemicellulosic hydrolysate for biotechnological production of xylitol by Candida guilliermondii

Cited by 29 publications

References 3 publications

Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production

Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production

Valorization of sunflower meal through the production of ethanol from the hemicellulosic fraction

Composición química, digestibilidad y cinética ruminal de la digestión de residuos agrícolas tratados con explosión de vapor

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