Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

Saranya, V; Shenbagarathai, R

doi:10.1590/s1517-83822011000300032

Cited by 38 publications

(10 citation statements)

References 9 publications

(9 reference statements)

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“…Multiple melting peaks are a common feature of semi-crystalline polymers (Wellen et al 2015). Multi-component PHAs, as observed in the present study, which have other alkanoate components or long side chains, tend to have a lower T m (Saranya and Shenbagarathai 2011;Sandhya et al 2013). Sharma et al (2017) reported that the copolymer PHBV produced by Pseudomonas putida had a T m of 137-170 °C, which is higher than the T m of PHBV observed in the present study.…”

Section: Dsc Analysissupporting

confidence: 43%

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Singh

Sitholé

Lekha

et al. 2021

Bioresour. Bioprocess.

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The accumulation of petrochemical plastic waste is detrimental to the environment. Polyhydroxyalkanoates (PHAs) are bacterial-derived polymers utilized for the production of bioplastics. PHA-plastics exhibit mechanical and thermal properties similar to conventional plastics. However, high production cost and obtaining high PHA yield and productivity impedes the widespread use of bioplastics. This study demonstrates the concept of cyclic fed-batch fermentation (CFBF) for enhanced PHA productivity by Bacillus thuringiensis using a glucose-rich hydrolyzate as the sole carbon source. The statistically optimized fermentation conditions used to obtain high cell density biomass (OD600 of 2.4175) were: 8.77 g L−1 yeast extract; 66.63% hydrolyzate (v/v); a fermentation pH of 7.18; and an incubation time of 27.22 h. The CFBF comprised three cycles of 29 h, 52 h, and 65 h, respectively. After the third cyclic event, cell biomass of 20.99 g L−1, PHA concentration of 14.28 g L−1, PHA yield of 68.03%, and PHA productivity of 0.219 g L−1 h−1 was achieved. This cyclic strategy yielded an almost threefold increase in biomass concentration and a fourfold increase in PHA concentration compared with batch fermentation. FTIR spectra of the extracted PHAs display prominent peaks at the wavelengths unique to PHAs. A copolymer was elucidated after the first cyclic event, whereas, after cycles CFBF 2–4, a terpolymer was noted. The PHAs obtained after CFBF cycle 3 have a slightly higher thermal stability compared with commercial PHB. The cyclic events decreased the melting temperature and degree of crystallinity of the PHAs. The approach used in this study demonstrates the possibility of coupling fermentation strategies with hydrolyzate derived from lignocellulosic waste as an alternative feedstock to obtain high cell density biomass and enhanced PHA productivity.

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Section: Dsc Analysissupporting

confidence: 43%

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Singh

Sitholé

Lekha

et al. 2021

Bioresour. Bioprocess.

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“…An engineered E.coli expressing phaC1 from Pseudomonas sp. was also found to produce PHAs from molasses: 45 higher PHA yield was achieved from molasses (75% of CDM) as compared to sucrose (65% of CDM).…”

Section: Phas From Waste Glycerolmentioning

confidence: 95%

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Favaro

Basaglia

Casella

2018

Biofuels Bioprod Bioref

118

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Polyhydroxyalkanoates (PHAs) are a family of biodegradable intracellular polyesters that a number of Eubacteria and Archaea can accumulate for energy and carbon storage. Most of the genetic modifications to the producing bacterial species have been accomplished to clarify basic biochemical, genetic, and metabolic aspects of PHA metabolism. However, due to its plastic‐like properties and complete biodegradability, this bio‐based polymer has attracted the attention of a variety of manufacturers. A number of genetic approaches have therefore been reported, aimed at improving the performance of the microorganisms with a potential for use in a production process. Indeed, genetic tools may find useful applications in all the phases of the PHA production chain, from the isolation and characterization of new microbial strains through all the production steps until they reach the downstream processes. The substrates generally used for PHA production are expensive, so the search for low‐cost feedstock is necessary. These materials, possibly deriving from agri‐food processes, are unfortunately not easily degraded or converted directly into PHAs. Thus, the development of engineered microbes is in progress to process waste streams and covert them to valuable polymers. This review will summarize the most relevant results obtained through genetic engineering tools for the production of PHAs from cheap carbon sources in view of possible industrial applications. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd.

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“…[326] Sinorhizobium meliloti 41 and Hydrogenophaga pseudoflava DSM 1034 can biosynthesize P(3HB), P(3HB-co-3HV) copolyesters, and P(3HB-co-3HV-co-4HB) terpolyesters from lactose. [329][330][331] Furthermore, PHAs can also be produced directly from molasses (e.g.,s ugarcane, sugar beet,a nd soy molasses), ahigh sucrose content byproduct from the sugar industry,o ro ther sucrose-rich products (e.g.,m aple sap, sugar beet juice, pineapple juice, or oil palm frondj uice) by using C. necator, [332] A. vinelandii, [331] Enterobacter species, [333] Pseudomonas corrugate, [334] A. latus, [335] recombinant E. coli, [336,337] B. subtilis, [337] B. megaterium, [338] Bacillus cereus, [339] or mixed cultures. [327] Sucrose, ad isaccharide composed of ag lucosea nd af ructose unit, is one of the most abundant and relativelyi nexpensive carbon sources extracted from sugar-bearing raw materials, such as sugar beet and sugarcane.…”

Section: Carbohydratesmentioning

confidence: 99%

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

2018

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Biobased polymers have seen their attractiveness increase in recent decades thanks to the significant development of biorefineries to allow access to a wide variety of biobased building blocks. Polyesters are one of the best examples of the development of biobased polymers because most of them now have their monomers produced from renewable resources and are biodegradable. Currently, these polyesters are mainly produced by using traditional chemical catalysts and harsh conditions, but recently greener pathways with nontoxic enzymes as biocatalysts and mild conditions have shown great potential. Bacterial polyesters, such as poly(hydroxyalkanoate)s (PHA), are the best example of the biotic production of high molar mass polymers. PHAs display a wide variety of macromolecular architectures, which allow a large range of applications. The present contribution aims to provide an overview of recent progress in studies on biobased polyesters, especially those made from short building blocks, synthesized through step-growth polymerization. In addition, some important technical aspects of their syntheses through biotic or abiotic pathways have been detailed.

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Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses

Cited by 38 publications

References 9 publications

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

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