Lignin degradation, ligninolytic enzymes activities and exopolysaccharide production by Grifola frondosa strains cultivated on oak sawdust

Abstract

Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains (MBFBL 21, 638 and 662) appeared to be good producers of exopolysaccharides (3.5, 3.5 and 3.2 mg/ml respectively)

Effect of different organic waste on cellulose-degrading enzymes secreted by Petriella setifera in the presence of cellobiose and glucose

“…For the last waste (BP) to be analysed, a higher content of hemicellulose (HCEL), and CA were detected whereas the contents of lignin and CF were determined to be at a lower level. Our findings were in accordance with other studies (Mikiashvili et al 2011;Stevenson et al 2012).…”

Biodegradation and Sugar Release from Canola Plant Biomass by Selected White Rot Fungi

“…A loss in organic matter of 34% was recorded for P. tuberregium, which was significantly different from the other tested WRF (23.6% -25.8%). This result recorded during this study is within the range (12.8% -34.1%) recorded for Grifola frondosa during the biodegradation of oak sawdust in a 55-day fermentation experiment [36] and was lower than 55.8% recorded for Lentinus squarrosulus with cornstalk after 30 days of incubation [16]. However, the fact that this level of degradation happened in P. tuberregium and other tested strains within three weeks indicates their rapid degradation capacity, which may be useful in the pretreatment/delignification of biomass prior to use in producing cellulosic ethanol and other biomass to bio-products applications.…”

“…L. squarrosulus produced polysaccharides attaining peak concentration of 4.0 and 5.13 mg/mL after 18 and 24 days of incubation [16]. Mikiashvilli et al [36] found that G. frondosa produced polysaccharide ranging 3.2 -3.5 mg/mL under SSF after 55 days of incubation. The roles of EPS secreted by fungi include mycelia adhesion to the substrate, protection against dehydration; and storage as a carbon source [21].…”

“…Their application is in nutraceuticals, especially β-glucans [43] and environmental remediation [44]. However, studies on exopolysaccharide production in lignocellulosic substrates under SSF have been few [16] [36].…”

Microbial Production of Extracellular Polysaccharides from Biomass Sources

“…Otherwise, it is utilized to a limited extend during the fermentation and hence requires pretreatments beforehand (Toksoy Oner 2013). Mikiashvili et al (2011) investigated 14 strains of Grifola frondosa for lignin degradation, ligninolytic enzyme activities, protein accumulation, and EPS production. Experiments were carried out in SSF using oak sawdust as substrate.…”