Multiresistance and endemic status of acinetobacter baumannii associated with nosocomial infections in a tunisian hospital: a critical situation in the intensive care units

Abstract

Acinetobacter baumannii is often implicated in hospital outbreaks in Tunisia. It’s a significant opportunistic pathogen associated with serious underlying diseases such as pneumoniae, meningitis and urinary tract infections. The aim of our study was to evaluate its degree of endemicity and its antibiotic resistance evolution essentially in the unit care where its isolation was predominant (57%).This study used 3 methods: antibiotyping, RAPD using 2 primers VIL 1, VIL5 and PFGE with ApaI restriction enzyme. The presence of integron1 and 2 was also studied.Antibiotyping showed that 92% of patients were resistant of all ß- lactams (except Imipenem) and that the resistance to Imipenem occurred in 47% of cases. RAPD profiles obtained with the 2 arbitrarily primers VIL1 and VIL5 gave respectively 5 and 4groups and PFGE fingerprinting patterns revealed 22 different pulsotypes. Integron 1 was present in 25% of unrelated strains and type 2 integron was not detected in any of the studied strains.Among 204 strains, multiple and heterogeneous groups were detected with the genomic studies. In addition, any correlation was obtained with the antibiotyping results. These findings demonstrate the endemic status of A. baumannii in our hospital and the persistence of a large number of multiresistant strains in the unit’s care. When outbreaks of A. baumannii occur, it’s essential to develop restricted hygiene procedures and a serious surveillance of critical units such as ICU for very ill patients.

OXA-Carbapenemases Present in ClinicalAcinetobacter baumannii-calcoaceticusComplex Isolates from Patients in Kurdistan Region, Iraq

“…Urology (1) n.a. Unknown (1) n.a. RoH (2) Burn unit (2) 2 (B2, B6) November 2013 C (9) RoH ( (1) n.a.…”

“…RoH (2) Burn unit (2) 2 (B2, B6) November 2013 C (9) RoH ( (1) n.a. RzH (1) Surgery (1) n.a. HaH (1) Surgery (1) n.a.…”

“…1,2 Infections caused by A. baumannii are difficult to treat because of its intrinsic resistance to various classes of antibiotics and its ability to acquire additional resistance. 3,4 Due to these characteristics, A. baumannii is included in the so-called group of ESKAPE organisms.…”

Molecular Characterization of Carbapenem Resistant Isolates of Acinetobacter baumannii in An Intensive Care Unit of A Tertiary Care Centre at Central India

“…Acinetobacter is non fermentative, gram-negative coccobacilli that has emerged as an important opportunistic pathogen, frequently occuring in critically ill intensive care unit (ICU) patients with chronic illness or prolonged hospitalizations [1,2]. A baumannii is ubiquitous in the hospital environment, particularly in the ICU and constitute 80 percent of the total clinical isolates of Acinetobacter that has been reported worldwide [3].…”

Imipenem-resistant Acinetobacter baumannii carrying the ISAba1-bla OXA-23, 51 and ISAba1-bla ADC-7 genes in Monteria, Colombia

“…Acinetobacter baumannii can cause a variety of infections including pneumonia, bacteremia, meningitis, urinary tract infections, peritonitis and infections of skin and soft tissue (2). Mortality is high in association with bacteremia (52%) and pneumonia (23–73% (6, 8).…”