2010
DOI: 10.1590/s1517-83822010000400003
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Abstract: Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as well as that antifungal resistance is associated with particular clades. These findings suggested that C. albicans reprod… Show more

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Cited by 8 publications
(13 citation statements)
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References 58 publications
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“…The distributions of sequence types between healthy subjects and periodontitis patients were significantly different. According to other reports, virulence factors and sites of isolation are associated with clade specificity [ 42 , 43 ]. However, it is not clear if the presence of C. albicans in the periodontal pockets contributes to the progression of periodontitis.…”
Section: Oral Microbiota Including Candida mentioning
confidence: 99%
“…The distributions of sequence types between healthy subjects and periodontitis patients were significantly different. According to other reports, virulence factors and sites of isolation are associated with clade specificity [ 42 , 43 ]. However, it is not clear if the presence of C. albicans in the periodontal pockets contributes to the progression of periodontitis.…”
Section: Oral Microbiota Including Candida mentioning
confidence: 99%
“…Molecular techniques include Cp3-13-specific probe, hybridization, PFGE (pulsed-field gel electrophoresis), RAPD (random amplification of polymorphic DNA), RFLP (restriction fragment length polymorphism), AFLP (amplified fragment length polymorphism) and MLST (multilocus sequence typing) [8,12,13]. These techniques have helped delineate subtypes of colonizing and/or infecting C. albicans strains over sequential evaluations of HIV-infected individuals.…”
Section: Introduction mentioning
confidence: 99%
“…Dentro de las técnicas más utilizadas en los últimos años para el estudio epidemiológico de C. albicans está Multilocus Sequence Typing (MLST), una herramienta que permite almacenar y compartir datos, por medio del análisis de polimorfismo de nucleótidos en fragmentos de genes conservados (Bougnoux, Tavanti et al 2003). Así mismo, esta técnica permite la identificación de clones y su ubicación en clados y complejos clonales ya definidos dentro del esquema MLST, lo cual ha sido útil en la investigación de características por las cuales se relaciona cada grupo, como áreas geográficas, resistencia a antifúngicos, virulencia y sitio de aislamiento (Lyon, Moraes et al 2010).…”
Section: Justificaciónunclassified
“…Estos análisis han demostrado que la distribución en clados está influenciada por áreas geográficas, resistencia a antifúngicos, virulencia y sitio de aislamiento. Esto sugiere que aunque el tipo de reproducción de C. albicans es principalmente clonal, también existe cierto grado de microvariación del ADN (Lyon, Moraes et al 2010).…”
Section: Relaciones Filogenéticas Entre Aislamientosunclassified
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