Multiple responses of gram-positive and gram-negative bacteria to mixture of hydrocarbons

Lazaroaie, Mihaela Marilena

doi:10.1590/s1517-83822010000300016

Cited by 51 publications

(21 citation statements)

References 79 publications

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“…Recently, Bastida et al (2016) reported that the resistance mechanism of Gram-negative bacteria to hydrocarbon was attributed to membrane modification (variations in membrane fluidity), whilst that of Grampositive was likely due to changes in biomass. Similarly, Lazaroaie (2010) attributed the ability of Gram-negative bacteria group to withstand pollutant in soil, especially PAH, to cell wall composition of this bacterial group. The isolation of alphaproteobacteria particularly Ochobactrum sp.…”

Section: Discussionmentioning

confidence: 99%

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

2017

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Acute and chronic pollution of environments with crude oil does not bode well for biota living within the vicinity of polluted environments. This is due to environmental and public health concerns on the negative impact of crude oil pollution on living organisms. Enhancing microbial activities by adding nutrients and other amendments had proved effective in pollutant removal during bioremediation. This study was carried out to determine how microbial group respond during remediation by enhanced natural attenuation (RENA) during a field-scale bioremediation. Crude oil-polluted soil samples were collected (before, during, and after remediation) from a site undergoing remediation by enhanced natural attenuation (RENA) at Ikarama Community, Bayelsa State, Nigeria, and were analyzed for total petroleum hydrocarbon (TPH), polyaromatic hydrocarbon (PAH), and a shift in microbial community. The gas chromatography-flame ionization detector (GC-FID) results showed that the pollutant concentrations (TPH and PAH) reduced by 98 and 85%, respectively, after the remediation. Culturable hydrocarbon utilizing bacteria (CHUB) was highest (8.3 9 10 4 cfu/g) for sample collected during the remediation studies, whilst sample collected after remediation had low CHUB (6

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Section: Discussionmentioning

confidence: 99%

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

2017

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“…They are generally associated with a fast petroleum degradation phase and are the more efficient (Ganesh and Lin 2009). Gram-positive bacteria such as Bacillus, Rhodococcus, Staphylococcus, and Exiguobacterium have also been found to be hydrocarbontolerant bacteria (Lazaroaie 2010).…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of diesel oil by a novel microbial consortium: comparison between co-inoculation with biosurfactant-producing strain and exogenously added biosurfactants

Mnif

Sahnoun

et al. 2015

Environ Sci Pollut Res

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Bioremediation, involving the use of microorganisms to detoxify or remove pollutants, is the most interesting strategy for hydrocarbon remediation. In this aim, four hydrocarbon-degrading bacteria were isolated from oil-contaminated soil in Tunisia. They were identified by the 16S rDNA sequence analysis, as Lysinibacillus bronitolerans RI18 (KF964487), Bacillus thuringiensis RI16 (KM111604), Bacillus weihenstephanensis RI12 (KM094930), and Acinetobacter radioresistens RI7 (KJ829530). Moreover, a lipopeptide biosurfactant produced by Bacillus subtilis SPB1, confirmed to increase diesel solubility, was tested to increase diesel biodegradation along with co-inoculation with two biosurfactant-producing strains. Culture studies revealed the enhancement of diesel biodegradation by the selected consortium with the addition of SPB1 lipopeptide and in the cases of co-inoculation by biosurfactant-producing strain. In fact, an improvement of about 38.42 and 49.65 % of diesel degradation was registered in the presence of 0.1 % lipopeptide biosurfactant and when culturing B. subtilis SPB1 strain with the isolated consortium, respectively. Furthermore, the best improvement, evaluated to about 55.4 %, was recorded when using the consortium cultured with B. subtilis SPB1 and A. radioresistens RI7 strains. Gas chromatography analyses were correlated with the gravimetric evaluation of the residual hydrocarbons. Results suggested the potential applicability of the selected consortium along with the ex situ- and in situ-added biosurfactant for the effective bioremediation of diesel-contaminated water and soil.

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“…Our results are consistent with earlier studies because most of the toluene metabolizing bacteria reported in literature are gram positive: Bacterium Ex-DG74 (17), Mycobacterium sp . IBB pol (23), Mycobacterium strains T103 and T104 (24). However, contrary to our findings previous studies have reported few toluene metabolizing gram negative bacteria (23).…”

Section: Discussionmentioning

confidence: 99%

Biochemical, molecular and antibiotic resistance profile of multi-potential toluene metabolizing bacteria isolated from tannery effluents

Muccee

Ejaz

2018

Preprint

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16The focus of present study was to isolate and characterize bacteria which can be 17 effectively used for toluene, a highly recalcitrant pollutant, bioremediation. For isolation 18 of bacteria from the tannery effluents selective enrichment and serial dilution methods 19 were employed. The isolated bacteria were subjected to growth curve analysis, estimation 20 of toluene removal efficiencies, biochemical tests, antibiotic sensitivity assays and 21 molecular characterization based upon 16S rRNA gene. The rRNA genes sequences were 22 analyzed through BLAST to determine similarity index of isolates with bacterial database 23 sequences. To trace the evolutionary history, phylogenetic trees were constructed using 24 MEGA version 7. Total twenty toluene metabolizing bacteria (4)(5)(6)(7)(8)(9)(10)(11)(12) 16, 19, 21, 25 23-26, 28 and 30) were isolated and characterized. Their rRNA gene sequences have 26 been submitted to Genbank. Fifteen of the twenty isolates showed homology to 27 2 Brevibacillus agri strain NBRC 15538, four found similar to Bacillus paralicheniformis 28 strain KJ-16 and one homologous to Burkholderia lata strain 383. All bacterial isolates 29 were resistant to chloramphenicol but sensitive to teicoplanin and linezolid. However, 30 few (i. e.; IUBT9 and 26) were sensitive to oxacillin. Biochemical characterization 31 indicated all bacteria positive for alkaline phosphatases (100%). While many were found 32 positive for p-nitrophenyl N-acetyl β, D-glucosaminidase (35%), hydroxyproline β-33 naphthylaminopeptidase (15%), esculinase (65%), mannitol (75%), sorbitol (95%) and 34 inulin (90%) fermentation. Biochemical profile suggests the use of isolated bacteria for 35 future exploitation in several fields like bioremediation of toluene, ethanol production, 36 biomass hydrolysis, biosensors, biofertilizers, as a marker for milk pasteurization in dairy 37 industries and evaluation of soil quality. 38 Importance 39Toluene is a highly toxic environmental pollutant. We have isolated bacteria which can 40 be effectively used for the removal of toluene from environmental resources. Moreover, 41 these bacteria are capable to produce many valuable enzymes which can be used in many 42 industrial processes for the production of a wide range of products. Further study may 43 help to exploit these bacterial for the benefit of humanity. 44 45 Key words: toluene metabolizing bacteria, bioremediation, toluene removal efficiency, 46 Brevibacillus agri, Bacillus paralicheniformis, Burkholderia lata, rRNA profiling 47 48 49Toluene (methylbenzene) is an omnipresent pollutant and is a cause of concern due to its 50 resistance to chemical, photolytic and biological degradation, lipophilic nature, 51 bioaccumulation, long-range transport and wide range adverse effects on environment, 52 wild life, biota and human health (1). Being lipophilic, it gets accumulated in lipid bilayer 53 of cell membrane and alters the structure of living cells (2). Toluene is a man-made 54 hydrocarbon and its major sources in urban air e...

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Multiple responses of gram-positive and gram-negative bacteria to mixture of hydrocarbons

Cited by 51 publications

References 79 publications

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

Shift in microbial group during remediation by enhanced natural attenuation (RENA) of a crude oil-impacted soil: a case study of Ikarama Community, Bayelsa, Nigeria

Biodegradation of diesel oil by a novel microbial consortium: comparison between co-inoculation with biosurfactant-producing strain and exogenously added biosurfactants

Biochemical, molecular and antibiotic resistance profile of multi-potential toluene metabolizing bacteria isolated from tannery effluents

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