Bioemulsifier production by Microbacterium sp. strains isolated from mangrove and their application to remove cadmiun and zinc from hazardous industrial residue

Abstract

The contamination of ecosystems with heavy metals is an important issue in current world and remediation technologies should be in according to environmental sustainability concept. Bioemulsifier are promising agents to be used in metal removal and could be effective to many applications in environmental industries. The aims of this work was screening the potential production of bioemulsifier by microorganisms isolated from an oil contaminated mangrove, and evaluate cadmium and zinc removal potential of those strains from a hazardous industrial residue. From that, bioemulsifier-producing bacteria were isolated from urban mangrove sediments. Four isolates were identified as Microbacterium sp by 16S rRNA analysis and were able to reduce up to 53.3% of culture medium surface tension (TS) when using glucose as carbon and energy source and 20.2% when sucrose was used. Suspensions containing bioemulsifier produced by Microbacterium sp. strains show to be able to remove cadmium and zinc from contaminated industrial residue, and its ability varied according carbon source. Significant differences in metal removal were observed by all strains depending on the carbon source. When glucose was used, Cd and Zn removal varied from 17 to 41%, and 14 to 68%, respectively. However, when sucrose was used it was observed only 4 to a maximum of 15% of Cd removal, and 4 to 17% of Zn removal. When the same tests were performed after ethanol precipitation, the results were different: the percentages of removal of Zn (7-27%) and Cd (14-32%) were higher from sucrose cultures. This is the first report of heavy metals removal by bioemulsifier from Microbacterium sp.

An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand

“…Excessive inputs from anthropogenic activities have caused serious contamination and adversely affect the health of aquatic and human life through bioaccumulation. PAHs are hydrophobic and readily adsorbed onto particulate matter; therefore, coastal and marine sediments become the ultimate sinks and elevated concentrations have been recorded (Aniszewski et al 2010). Solubilization of PAHs depends on the type and dose of the surfactant, the hydrophobicity, the surfactant-soil interactions and the time that a contaminant has been in contact with the soil (Zhou and Rhue 2000).…”

Bioremediation of Mangroves Impacted by Petroleum

“…They are also reservoirs of products of biotechnological interest (e.g. bacteria that produce bioemulsifiers) (Aniszewski et al 2010).…”

Búsqueda De Capacidad Productora De Biosurfactantes en Actinobacterias Haloalcalófilas Y Haloalcalotolerantes

“…Sin embargo, hasta el momento no hay reportes que indiquen este comportamiento. Aniszewski et al (2010) De manera similar ocurre con la especie Nocardiopsis salina, en este trabajo se reporta la capacidad de dos cepas en producir biosurfactante con propiedades emulsificantes, además de crecer en presencia de antraceno y no existen reportes similares. Otros autores demostraron la capacidad de producir biosurfactante y bioemulsificante en otras especies de este género como Nocardiopsis alba (Gandhimathi et al 2009) y Nocardiopsis lucentensis (Kiran et al 2010).…”

“…Algunas especies de actinobacterias productoras de biosurfactante son: Kocuria rosea (Mahjoubi et al 2013), Nocardia otitidiscaviarum (Vyas y Dave 2011), Microbacterium sp. (Aniszewski et al 2010), Nocardiopsis alba (Gandhimathi et al 2009) y Nocardiopsis lucentensis (Kiran et al 2010 (Bonnet et al 2005).…”