Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil

Cursino, Luciana; Muro-Abad, Júpiter Israel; Gomes, E. A.; Araújo, Elza Fernandes de; Hungría, Mariangela; Cassini, Sérvio Túlio Alves

doi:10.1590/s1517-83822009000400016

Cited by 14 publications

(7 citation statements)

References 14 publications

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“…This fast and relatively cheap method has been widely used in genetic variability studies of rhizobial species [10, 16-17, 20, 27]. An increasing emphasis is placed on genetic diversity of local, indigenous rhizobial strains, several more in-depth methods being employed [2,[4][5]23].…”

Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

RAPD-inferred genetic variability of some indigenousRhizobium leguminosarumisolates from red clover (Trifolium pratenseL.) nodules

Stefan

Roșu

Stedel

et al. 2015

Acta Biologica Hungarica

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The application of commercial rhizobial inoculants to legume crops is proving to be an alternative to synthetic fertilizer use. The challenge for sustainable agriculture resides in the compatibility between crop, inoculants and environmental conditions. The evaluation of symbiotic efficiency and genetic diversity of indigenous rhizobial strains could lead to the development of better inoculants and increased crop production. The genetic variability of 32 wild indigenous rhizobial isolates was assessed by RAPD (Random Amplified Polymorphic DNA). The strains were isolated from red clover (Trifolium pratense L.) nodules from two distinct geographical regions of Northern and Eastern Romania. Three decamer primers were used to resolve the phylogenetic relationships between the investigated isolates. Cluster analysis revealed a high diversity; most strains clustered together based on their geographical location.

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Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

RAPD-inferred genetic variability of some indigenousRhizobium leguminosarumisolates from red clover (Trifolium pratenseL.) nodules

Stefan

Roșu

Stedel

et al. 2015

Acta Biologica Hungarica

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“…A maioria dos solos com histórico de cultivo de feijão contém rizóbios nativos que podem interferir no estabelecimento e na eficácia das estirpes inoculadas, reduzindo as respostas à inoculação (Vlassak et al, 1996;Vargas et al, 2000;Raposeiras et al, 2006 (Mostasso et al, 2002;Raposeiras et al, 2006), na Região Sul Hungria et al, 2003;Stocco et al, 2008) e na Região Sudeste (Soares et al, 2006;Lombardi et al, 2009;Torres et al, 2009). Além dessas estirpes de Rhizobium, grande número de isolados classificados como Sinorhizobium sp.…”

Section: Introductionunclassified

Resposta do Feijoeiro Comum à Inoculação com Rizóbio e Suplementação com Nitrogênio Mineral em Dois Biomas Brasileiros

Brito

Pacheco

Filho

et al. 2015

Rev. Bras. Ciênc. Solo

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O feijoeiro comum (Phaseolus vulgaris L.) pode se beneficiar da fixação biológica de N2, mas respostas inconsistentes da cultura à inoculação com rizóbio indicam a necessidade de aplicação de N mineral complementar. Este trabalho teve por objetivo avaliar a resposta do feijoeiro à inoculação com rizóbio, associada à suplementação com N mineral, nos biomas Cerrado e Mata Atlântica. Foram conduzidos quatro experimentos de campo, dois em Santo Antônio de Goiás, GO, um em Valença, RJ e um em Macaé, RJ, onde a inoculação com estirpes comerciais de rizóbio foi comparada à inoculação com a estirpe BR 923 de Sinorhizobium sp., à adubação com N mineral e à suplementação com N na semeadura e em cobertura. A avaliação da população nativa de rizóbio indicou 105 células g-1 no solo na área experimental em Goiás, anteriormente cultivada com feijão, e 102células g-1 em Valença, anteriormente mantida com pastagem. Nos dois experimentos em Goiás, o rendimento de grãos, da ordem de 2.100 kg ha-1, não diferiu entre os tratamentos testemunha absoluta, inoculação com rizóbio ou aplicação de 120 kg ha-1 de N. Em Valença, a inoculação com estirpes comerciais forneceu rendimentos da cultivar Ouro Negro superiores à testemunha absoluta, na ausência de adubação de cobertura; na presença de 40 kg ha-1 de N em cobertura, a inoculação com rizóbio proporcionou rendimento de 3.420 kg ha-1, superior aos demais tratamentos. Na média das diferentes fontes de N na semeadura, a adubação de cobertura aumentou a produção de grãos de 2.367 para 2.542 kg ha-1. Em Macaé, em solo com alto teor de matéria orgânica, os maiores rendimentos foram obtidos com inoculação das estirpes comerciais associada a 40 kg ha-1 de N em cobertura, com efeito deletério da adubação de 80 kg ha-1 de N no plantio. Concluiu-se que em áreas sem cultivo prévio de feijão, a inoculação com estirpes comerciais de rizóbio aumentou o rendimento de grãos, em particular quando associada à adubação de cobertura com N.

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“…Phaseolus vulgaris yield is low in East Africa, mainly due low soil fertility with most of the soils having low available nitrogen and phosphorus [25]. Like other leguminous plants this plant is able to establish nitrogen fixing symbiotic relationship with rhizobia, which can improve the crop yield [22,26]. P. vulgaris is described as promiscuous in its symbiotic interactions, because it has the ability of nodulating to nodulate with a diversity of rhizobial species [27,28] [27].…”

Section: Introductionmentioning

confidence: 99%

Morphological Assessment and Effectiveness of Indigenous Rhizobia Isolates that Nodulate P. vulgaris in Water Hyacinth Compost Testing Field in Lake Victoria Basin

Muthini

2014

BJAST

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Aims: The study was aimed at isolating, identifying and assessing the effectiveness of indigenous rhizobia nodulating P. vulgaris in Lake Victoria Basin (LVB). Study design: Randomized complete block design. Place and Duration of Study: Soil and nodule samples were collected from Kisumu (Kenya); Kabanyolo (Uganda) and Nyabarongo (Rwanda). Field experiments: Kisumu (Kenya). Lab and greenhouse experiments: Department of Plant and Microbial Sciences Kenyatta University (Kenya) and Makerere University (Uganda). Research was carried out between January 2012 and April 2013. Methodology: Rhizobia were isolated from nodules obtained from P. vulgaris (rose coco variety) plants planted in the LVB water hyacinth compost trial fields and whole soil trapping experiments in the greenhouse using soil obtained from the LVB. The isolates were identified using morphological characteristics. Isolates from each group were used in authentication using the infection technique. Results: One hundred and twenty eight isolates were obtained from the trapping experiments and placed into nine groups based on their morphological characteristics. Four hundred and seventy two isolates were obtained from the nodules of the P. vulgaris grown in soils amended with water hyacinth compost and were placed into sixteen groups. The isolates varied in their morphological characteristics. There was a significant difference in the infectiveness and effectiveness of the representative rhizobia isolates. Conclusion: The studies revealed that rhizobia isolates from Lake Victoria are different morphologically. Authentication experiments, confirmed that the majority of the isolates were rhizobia due to their ability to infect the host plant P. vulgaris. All representative isolates varied in their ability to infect and fix nitrogen. Isolates that are more effective compared to the commercial Rhizobium leguminosarum biovar phaseoli strain 446 were identified in this study. The effective indigenous rhizobia have therefore the potential of being sources of inocula for P. vulgaris.

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Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil

Cited by 14 publications

References 14 publications

RAPD-inferred genetic variability of some indigenousRhizobium leguminosarumisolates from red clover (Trifolium pratenseL.) nodules

RAPD-inferred genetic variability of some indigenousRhizobium leguminosarumisolates from red clover (Trifolium pratenseL.) nodules

Resposta do Feijoeiro Comum à Inoculação com Rizóbio e Suplementação com Nitrogênio Mineral em Dois Biomas Brasileiros

Morphological Assessment and Effectiveness of Indigenous Rhizobia Isolates that Nodulate P. vulgaris in Water Hyacinth Compost Testing Field in Lake Victoria Basin

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