Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India

Abstract

Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus.

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

“…The majority of the genera and species recovered in this study were from marsh sediments and have previously been described in saline environments except B. safensis, Bacillus thioparans and B. simplex (Ventosa et al, 1998;Okamoto et al, 2001;Yoon et al, 2001;2003;Chookietwattana, 2003;Sánchez-Porro et al, 2003;Borsodi et al, 2005;Fan et al, 2006;Pérez-Ibarra et al, 2007;Ventosa et al, 2008;Parvathi et al, 2009;Sasaki et al, 2009;Zhang et al, 2010;Bassey and Grigson, 2011). This finding extends our knowledge of the bacterial diversity in saline environments and indicates that the groups obtained, including potential new species, are well adapted.…”

Purification and characterization of β $$ \boldsymbol{\upbeta} $$ -mannanase from Bacillus pumilus (M27) and its applications in some fruit juices

“…Later on, in consequence of the analyses performed by utilizing the API 50CHB test system, this isolate was identified to be the Bacillus pumilus (data not shown). These results obtained were determined to comply with the literature data (Bottone and Peluso 2003;Parvathi et al 2009;Liu et al 2013). …”

Phylogenetic and clonality analysis ofBacillus pumilusisolates uncovered a highly heterogeneous population of different closely related species and clones

“…Therefore, B. pumilus was not the most widespread bacterial group among our terrestrial B. pumilus group isolates collection, in contrast to general conception that considered this species as the pre-dominant one (Newcombe et al, 2005;Parvathi et al, 2009). A more close relation with B. altitudinis (gyrB 3 ) (genetic distance of 0.086) and B. invictae (gyrB 3 ) (genetic distance of 0.074) than with B. xiamenensis HYC-10 T (genetic distance of 0.107) was observed.…”