Screening of different fungi for decolorization of molasses

Seyis, Isil; Subasioglu, Tugba

doi:10.1590/s1517-83822009000100009

Cited by 20 publications

(16 citation statements)

References 16 publications

(28 reference statements)

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“…Experiment was repeated with the optimized parameters and COD reduction with residual color was observed. The observance was measured as decrease in optical den sity of supernatant of treated ADSW medium at 475 nm (Krishna Prasad et al, 2009;Seyis, et al, 2009) on UV Spectrophotometer (Perkin Elmer Model: Lambda 35). pH determination was made by using Digital portable pH meter (Model pt-10P, Sartorius).…”

Section: Methodsmentioning

confidence: 99%

“…In case of decolorization of molasses Trichoderma species it was concluded that enzymes formed by fungi during the process were effective only in acidic conditions. (Seyis et al, 2009) An increase in color at high pH was due to the polymerization of melanoidins and higher nutrient utilization. However, overall significant decolorisation was obtained in the optimal range of 5-6 which confirms the significant rol of pH in color removal.…”

Section: Effect Of Ph On Color Removalmentioning

confidence: 99%

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Single factorial experimental design for decolorizing anaerobically treated distillery spent wash using cladosporium cladosporioides

Rajarathinam¹,

Vasanthi²,

Saravanan³

2010

Int. J. Environ. Sci. Technol.

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ABSTARCT:This study presents the standardization of nutrient concentration, pH and temperature required to decolorize the anerobically treated distillery spent wash using the fungus Cladosporium cladosporioides. Experiments were carried out to measure the decolorization of distillery spent wash effluent and it was found to be effective in acidic environment. From the results it was observed that a maximum color reduction of 52.6 % and Chemichal Oxygen Demand. removal of 62.5 % were achieved. The optimum conditions required for the growth of the fungus was found to be 5 g/L of fructose, 3 g/L of peptone, 5 pH and 35 °C. It was also observed that during the process a maximum of 1.2 g of fungal growth was attained. Decolorizing ability of the fungus was confirmed using spectrophotometer and High Performance Liquid Chromatography analysis. Single factorial experimental design was used to optimize the parameters. Apart from decolorization it was observed that fungus also has the ability to degrade the spent wash efficiently. This investigation could be an approach towards control of environmental pollution and health hazards of people in and around the distillery unit.

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Section: Methodsmentioning

confidence: 99%

Section: Effect Of Ph On Color Removalmentioning

confidence: 99%

Single factorial experimental design for decolorizing anaerobically treated distillery spent wash using cladosporium cladosporioides

Rajarathinam¹,

Vasanthi²,

Saravanan³

2010

Int. J. Environ. Sci. Technol.

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“…Melanoidin decolorization was studied at different range of pH (3.0-7.0) by various workers and have reported that enzymes formed by microorganism during the decolorization was effective only in acidic conditions (Seyis and Subasing, 2009). At high pH, increase in the color was due to the polymerization of melanoidins and higher nutrient utilization (Adikane et al, 2006;Jiranuntipon et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Decolorization of Distillery Effluent by Thermotolerant <i>Bacillus subtilis</i>

Tiwari¹

2012

American Journal of Applied Sciences

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Problem statement: Ethanol production from sugarcane molasses generate large volume of effluent containing high Biological Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) along with melanoidin, a color compound generally produced by "Millard reaction". Melanodin is a recalcitrant compound degraded by specific microorganisms having ability to produce mono and dioxygenases peroxidases, phenoxidases and laccases, are mainly responsible for degradation of complex aromatic hydrocarbons like color compound. These compounds causes several toxic effects on living system, therefore may be treated before disposal. Approach: The purpose of this study was to isolate a potential thermotolerant melanoidin decolorizing bacterium from natural resources for treatment of distillery effluent at industrial level. Results: Total 10 isolates were screened on solid medium containing molasses pigments. Three potential melanoidin decolorizing thermotolerant bacterial isolates identified as Bacillus subtilis, Bacillus cereus and Pseudomonas sp. were further optimized for decolorization at different physico-chemical and nutritional level. Out of these three, Bacillus subtilis showed maximum decolorization (85%) at 45°C using (w/v) 0.1%, glucose; 0.1%, peptone; 0.05%, MgSO 4 ; 0.01%, KH 2 PO 4 ; pH-6.0 within 24h of incubation under static condition. Conclusion/Recommendations: The strain of Bacillus subtilis can tolerate higher temperature and required very less carbon (0.1%, w/v) and nitrogen sources (0.1%, w/v) in submerged fermentation. It can be utilized for melanoidin decolorization of distillery effluent at industrial scale.

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“…Biomass production was evaluated by determining the dye mass of the mycelia. Mycelia were harvested from the cultivation liquid medium by filtration using filter paper, dried at 50°C overnight until a constant weight was achieved, and then weighed (Abedin 2008;Maximo et al 2003;Seyis and Subasioglu 2009).…”

Section: Decolorization Studies In Batch Liquid Mediummentioning

confidence: 99%

“…Some dyes are toxic, mutagenic, carcinogenic, or allergenic (Bakshi et al 1999). It is estimated that about 10-15% of dyes are released into processing water (Selvam et al 2003;Shakibaie et al 2008;Seyis and Subasioglu 2009;Singh and Singh 2010).…”

Section: Introductionmentioning

confidence: 99%

Bioremoval of an azo textile dye, Reactive Red 198, by Aspergillus flavus

Esmaeili

Kalantari

2011

World J Microbiol Biotechnol

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The objective of this paper was to study the potential for bioremoval of a textile dye, Reactive Red 198 (RR198), by a fungus isolated from soil collected from an effluent disposal area near a textile company. The fungus was identified as Aspergillus flavus, and its use as a low-cost live-cell biomass for the biodegradation of RR198 from contaminated water was investigated using batch studies. The effects of time, dye concentration, and pH as variable factors were examined in the process. Results showed that bioremoval of RR198 by A. flavus increased to over 84.96% with increasing time until equilibrium was reached after a period of 24 h. A low pH was the most effective, as were lower levels of dye concentration. The decolorization was determined by the decrease in the absorption maximums of this dye by UV-visible spectroscopy. A. flavus was shown to be an efficient fungus for removal of RR198 from wastewater.

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Screening of different fungi for decolorization of molasses

Cited by 20 publications

References 16 publications

Single factorial experimental design for decolorizing anaerobically treated distillery spent wash using cladosporium cladosporioides

Single factorial experimental design for decolorizing anaerobically treated distillery spent wash using cladosporium cladosporioides

Decolorization of Distillery Effluent by Thermotolerant <i>Bacillus subtilis</i>

Bioremoval of an azo textile dye, Reactive Red 198, by Aspergillus flavus

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