A toxic cyanobacterial bloom in an urban coastal lake, Rio Grande do Sul state, Southern Brazil

Carvalho, Luciana Retz de; Pípole, Fernando; Werner, Vera Regina; Laughinghouse, Haywood Dail; Camargo, Antônio Carlos Martins de; Rangel, Marisa; Konno, Katsuhiro; Anna, Célia Leite Sant'

doi:10.1590/s1517-83822008000400031

Cited by 29 publications

(14 citation statements)

References 20 publications

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“…Freshwater habitats in subtropical Brazil are relatively poorly known from a phycological perspective, although freshwater cyanobacteria from Rio Grande do Sul State have been documented by Callegaro et al (1981), Torgan et al (1981Torgan et al ( , 1995, Franceschini (1983Franceschini ( , 1990, Werner (1984Werner ( , 1988Werner ( , 2002, Torgan & Garcia (1989), Werner & Rosa (1992), Torgan & Paula (1994), Garcia & Vélez (1995), Torgan (1997), Werner & Sant'Anna (1998, 2006, Cardoso & Motta-Marques (2003, Carvalho et al (2008), Werner et al (2008), and Werner & Laughinghouse (2009)…”

Section: Introductionmentioning

confidence: 90%

Cyanobacteria from coastal lagoons in southern Brazil: non-heterocytous filamentous organisms

2012

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-(Cyanobacteria from coastal lagoons in southern Brazil: non-heterocytous fi lamentous organisms). This study describes and illustrates non-heterocytous fi lamentous cyanobacteria found in lagoon systems on the coastal plains of Rio Grande do Sul State. Collections were carried out in different freshwater bodies along the eastern (Casamento Lake area) and western (Tapes City area) margins of the Patos Lagoon (UTM 461948-6595095 and 542910-6645535) using a plankton net (25 μm mesh) in pelagic and littoral zones as well as by squeezing submerged parts of aquatic macrophytes, during both the rainy and dry seasons, from May to December/2003. Twenty two species belonging to the families Phormidiaceae (eight taxa), Pseudanabaenaceae (seven taxa), Oscillatoriaceae (six taxa), and Spirulinaceae (one taxon) were identifi ed. Among these species, fi ve are reported for the fi rst time from Rio Grande do Sul State: Leptolyngbya cebennensis, Microcoleus subtorulosus, Oscillatoria cf. anguina, O. curviceps and Phormidium formosum.

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Section: Introductionmentioning

confidence: 90%

Cyanobacteria from coastal lagoons in southern Brazil: non-heterocytous filamentous organisms

2012

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“…The release of cyanotoxins, such as microcystins, saxitoxins, anatoxin-a, anatoxin-a( S ), and cylindrospermopsins, in water reservoirs that are used for human consumption can cause public health problems [16,18,19,20,21]. These substances can be the source of acute lethal intoxications or may induce the development of chronic diseases, e.g., the continuous ingestion of sub-lethal doses of microcystins promotes the growth of hepatic tumors or necrosis [22,23].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

et al. 2014

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Cyanobacteria are common members of the freshwater microbiota in lakes and drinking water reservoirs, and are responsible for several cases of human intoxications in Brazil. Pseudanabaena galeata and Geitlerinema splendidum are examples of the toxic species that are very frequently found in reservoirs in Sao Paulo, which is the most densely populated area in Brazil. In the search for toxic strains collected from water reservoirs and maintained in the Cyanobacterial Culture Collection (CCIBt) of the Institute of Botany of Brazil, the acetic acid extracts (AE) of P. galeata CCIBt 3082 and G. splendidum CCIBt 3223 were analyzed by planar chromatography, which indicated the absence of cyanotoxins. Animal tests were then carried out, and both extracts were found to induce toxic effects in mice when administered intraperitoneally. The present study aimed to investigate whether the oral ingestion of the above mentioned cyanobacteria extracts would also induce toxic effects in mice. Necropsy and histopathological studies were conducted using tissue samples from the animals, which were euthanized one week after the administration of the extracts. The AE of P. galeata did not cause death but did induce transient symptoms, including eyebrow ptosis, straub tail, and pain. The euthanized animals presented hemorrhage in the liver, whereas the histological analysis showed disorganization of the hepatic parenchyma, necrosis, hyperemia, and proximity of the centrilobular vein in the liver. In addition, alterations in the convoluted tubules of the kidneys were observed, and the lungs were unaffected. The AE of G. splendidum caused only one death, and induced transient symptoms, such as dyspnea, paralysis, and pain, in the other mice. The necropsy of the euthanized mice showed hemorrhage in the lungs and liver. The lungs presented hemorrhagic focuses, alveolar collapse, and granulomatous foci. The liver presented hemorrhagic and enlarged sinusoids, hyperemia, proximity of the centrilobular vein, and disorganization of the hepatic parenchyma. Some areas also exhibited an inflammatory infiltrate and calcified tissue inside blood vessels. Necrosis and rupture of the convoluted tubule cells were observed in the kidneys. Further analysis of the both extracts indicated the lack of hemolytic activity, and the presence of two unknown anti-AChE substances in the AE of G. splendidum. Thus, P. galeata and G. splendidum are producers of novel toxins that affect mammals when administered orally.

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“…Carvalho et al(2008) described a cyanobacterial bloom in a southern Brazilian reservoir with Microcystis protocystis and Sphaerocavum cf. However, there have been few reports documenting the co-occurrence of other bioactive peptides.…”

Section: Introductionmentioning

confidence: 99%

Co-occurrence of microcystin and microginin congeners in Brazilian strains ofMicrocystissp.

Carneiro

Dörr

Bortoli

et al. 2012

FEMS Microbiol Ecol

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Species of Microcystis are the most common bloom‐forming cyanobacteria in several countries. Despite extensive studies regarding the production of bioactive cyanopeptides in this genus, there are limited data on isolated strains from Brazil. Three Microcystis sp. strains were isolated from the Salto Grande Reservoir (LTPNA01, 08 and 09) and investigated for the presence of mcy genes, microcystins and other cyanopeptides. Microcystin and microginin production was confirmed in two isolates using high‐resolution tandem mass spectrometry after electrospray ionization (ESI‐Q‐TOF), and the structures of two new microginin congeners were proposed (MG756 Ahda‐Val‐Leu‐Hty‐Tyr and MG770 MeAhda‐Val‐Leu‐Hty‐Tyr). The biosynthesis profile of the identified cyanopeptides was evaluated at different growth phases via a newly developed HPLC‐UV method. Results demonstrated no substantial differences in the production of microcystins and microginins after data normalization to cell quota, suggesting a constitutive biosynthesis. This study represents the first confirmed co‐production of microginins and microcystins in Brazilian strains of Microcystis sp. and highlights the potential of Brazilian cyanobacteria as a source of natural compounds with pharmaceutical interest.

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A toxic cyanobacterial bloom in an urban coastal lake, Rio Grande do Sul state, Southern Brazil

Cited by 29 publications

References 20 publications

Cyanobacteria from coastal lagoons in southern Brazil: non-heterocytous filamentous organisms

Cyanobacteria from coastal lagoons in southern Brazil: non-heterocytous filamentous organisms

Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

Co-occurrence of microcystin and microginin congeners in Brazilian strains ofMicrocystissp.

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