Detection of oral streptococci in dental biofilm from caries-active and caries-free children

Silva, Andréa Cristina Barbosa da; Cruz, Jáder Santos; Sampaio, Fábio Correia; Araújo, Demétrius Antônio Machado de

doi:10.1590/s1517-83822008000400009

Cited by 14 publications

(7 citation statements)

References 27 publications

(23 reference statements)

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“…Thus, the subsequent step was to evaluate the antibacterial properties of all wines obtained during the winemaking process against S. mutans , S. salivarius and S. pyogenes . These bacterial strains were chosen for the study with the specific end of assessing the action of wine against different streptococci of the oral cavity, directly or indirectly related to caries development [ 34 , 35 ]. All wine samples were dealcoholized before microbiological tests, with the aim of excluding ethanol interference on streptococcal growth.…”

Section: Resultsmentioning

confidence: 99%

“…Recent studies show that a moderate consumption of red wine is also related to the protection of oral health. In particular, Daglia et al tested the antibacterial activity of two commercial wines against oral streptococci responsible directly ( S. mutans ) or indirectly ( S. salivarius ) for the development of caries, and against S. pyogenes responsible for pharyngitis [ 12 , 34 , 35 ]. The tested red and white wines were active against tested strains.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Winemaking on the Composition of Red Wine as a Source of Polyphenols for Anti-Infective Biomaterials

et al. 2016

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Biomaterials releasing bactericides have currently become tools for thwarting medical device-associated infections. The ideal anti-infective biomaterial must counteract infection while safeguarding eukaryotic cell integrity. Red wine is a widely consumed beverage to which many biological properties are ascribed, including protective effects against oral infections and related bone (osteoarthritis, osteomyelitis, periprosthetic joint infections) and cardiovascular diseases. In this study, fifteen red wine samples derived from grapes native to the Oltrepò Pavese region (Italy), obtained from the winemaking processes of “Bonarda dell’Oltrepò Pavese” red wine, were analyzed alongside three samples obtained from marc pressing. Total polyphenol and monomeric anthocyanin contents were determined and metabolite profiling was conducted by means of a chromatographic analysis. Antibacterial activity of wine samples was evaluated against Streptococcus mutans, responsible for dental caries, Streptococcus salivarius, and Streptococcus pyogenes, two oral bacterial pathogens. Results highlighted the winemaking stages in which samples exhibit the highest content of polyphenols and the greatest antibacterial activity. Considering the global need for new weapons against bacterial infections and alternatives to conventional antibiotics, as well as the favorable bioactivities of polyphenols, results point to red wine as a source of antibacterial substances for developing new anti-infective biomaterials and coatings for biomedical devices.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Winemaking on the Composition of Red Wine as a Source of Polyphenols for Anti-Infective Biomaterials

et al. 2016

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“…However, the etiology of dental caries is still disputed due to development of dental caries even in the absence of S. mutans [17]. Sometimes mutans Streptococci cannot explain occurrence of caries in certain population [18,19]. Therefore, there is continuous controversy regarding involvement of bacteria in the etiology of dental caries.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of bacteria associated with dental caries with special reference to their etiologic potential

Khan¹,

Jain²,

Shrivastav³

2018

IJSRBS

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The dental caries is a significant public health problem affecting a number of children and adults throughout the globe. However, the prevalence of dental caries is increasing with increasing consumption of dietary sugar but several factors other than sugar are suggested for the etiology of dental caries including involvement of microorganisms and host factors. The microbes involved in dental caries convert dietary sugar into acid which lead to demineralization of tooth enamel and ultimately results in development of dental caries. The mutans streptococci including S. mutans are suggested to be involved in the etiology of dental caries. Under certain circumstances dental caries can develop even in the absence of these organisms. Due to the disparity in the etiology of this disease, we designed the study to isolate microbes from dental caries and compared them with microbes isolated from healthy individuals. The results revealed that although mutans streptococci is a major contributor in the etiology of dental caries, but some other non-mutans organisms can also contribute to dental caries etiology. The results suggest that proper consideration for removal of non mutans cariogenic bacteria should be given in addition to removal of mutans streptococci for management of dental caries. The results also suggest that some future studies focusing on removal of non mutans organism, mutans streptococci and both kind of organisms and their subsequent effects on management of dental caries should be done in order to understand relative contribution of these organisms in disease etiology.

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“…Among them, PCR provides a rapid, sensitive and specific technique [12,13]. So, in this study, qPCR technique was used for the rapid identification and quantification of S. mutans in salivary samples utilizing the LightCycler 480 Real-Time PCR System.…”

Section: Resultsmentioning

confidence: 99%

Quantitative detection of <i>Streptococcus mutans</i> from saliva using FTA<sup>TM</sup> elute cards and real-time polymerase chain reaction

Seghatoleslami¹,

Ohlsson²,

Hamberg³

et al. 2013

AJMB

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Dental caries is a localized, transmissible, pathological infection process that ends up in the destruction of hard dental tissue. Numerous reports have shown the close relationship between salivary levels of Streptococcus mutans and dental caries. As S. mutans, is considered to be the principle etiological agent of dental caries, the development of a quick and convenient method for detection and quantification of these bacteria from patient saliva samples would simplify diagnosis and treatment. The purpose of this study was to compare a new means of quantifying bacteria using FTA TM Elute cards and Real-Time Polymerase Chain Reaction to a conventional culture-based assay using oral S. mutans as a model sample. A total of 60 different saliva samples were investigated. The results show a significant negative correlation between the two methods, with a correlation coefficient of −0.577 (Spearman's Correlation) and p < 0.01. The method demonstrates a high sensitivity, specificity and reliable quantitative results, covering a large range of bacterial concentrations.

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Detection of oral streptococci in dental biofilm from caries-active and caries-free children

Abstract: This work correlated the presence of oral streptococci in dental biofilm with clinical indexes of caries and oral hygiene in caries-active and caries-free children. S. mutans and/or S. sobrinus in the dental biofilm does not indicate a direct risk for developing dental caries.

Cited by 14 publications

References 27 publications

Effect of Winemaking on the Composition of Red Wine as a Source of Polyphenols for Anti-Infective Biomaterials

Effect of Winemaking on the Composition of Red Wine as a Source of Polyphenols for Anti-Infective Biomaterials

Isolation and characterization of bacteria associated with dental caries with special reference to their etiologic potential

Quantitative detection of <i>Streptococcus mutans</i> from saliva using FTA<sup>TM</sup> elute cards and real-time polymerase chain reaction

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Detection of oral streptococci in dental biofilm from caries-active and caries-free children

Abstract: This work correlated the presence of oral streptococci in dental biofilm with clinical indexes of caries and oral hygiene in caries-active and caries-free children. S. mutans and/or S. sobrinus in the dental biofilm does not indicate a direct risk for developing dental caries.

Cited by 14 publications

References 27 publications

Effect of Winemaking on the Composition of Red Wine as a Source of Polyphenols for Anti-Infective Biomaterials

Effect of Winemaking on the Composition of Red Wine as a Source of Polyphenols for Anti-Infective Biomaterials

Isolation and characterization of bacteria associated with dental caries with special reference to their etiologic potential

Quantitative detection of &lt;i&gt;Streptococcus mutans&lt;/i&gt; from saliva using FTA&lt;sup&gt;TM&lt;/sup&gt; elute cards and real-time polymerase chain reaction

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Quantitative detection of <i>Streptococcus mutans</i> from saliva using FTA<sup>TM</sup> elute cards and real-time polymerase chain reaction