Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil

Abstract

We determined the frequency of hepatitis C virus (HCV) genotypes in anti-HCV seropositive patients in the state of Alagoas, Brazil, by means of nested-reverse transcription-polymerase chain reaction (RT-nested-PCR) followed by restriction fragment length polymorphism (RFLP) of amplified fragments of the 5´NCR. The nested-PCR with genotype-specific primers from the core region was carried out when detection was not possible by the first approach. Detectable HCV-RNA was present in 115 (74.7%) of 154 serum samples. Genotype 1 was the most frequent (77.4%), against 20.9% of genotype 3 and 0.8% of genotype 2. Subtype 1b was predominant (65.2%), followed by subtypes 1a (8.7%), and 3a (6.1%). Coinfection (1a/3a) was detected in 0.8% of the samples. Indeed, there was no significant differences in the prevalence of genotype 1 compared to what has been obtained from anti-HCV seropositive patients from other locations in Brazil. Here we report for the first time the genotype 2 in the state of Alagoas.

Hepatitis C screening, diagnosis, and cascade of care among people aged > 40 years in Brasilia, Brazil

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Serological Assay and Genotyping of Hepatitis C Virus in Infected Patients in Zanjan Province

“…A research in central Italy was reported genotype 4 as the prevalent genotype ( 20 ). Gonzaga RM et al investigated the distribution of hepatitis C virus genotypes in seropositive patients in Brazil in 2008 ( 40 ). The prevalence of different HCV genotypes in the world are as follows: subtype 1a is mostly reported in North America and Europe.…”

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“…Trop 44(5):638-640, set-out, 2011 prevalência do subtipo 1b (65,2%) foi maior do que a observada em Salvador, Bahia, onde este subtipo só ocorreu em 38,6% das amostras positivas para o anti-VHC. No entanto, foi muito semelhante aos resultados obtidos a partir de amostras de VHC positivas de outros Estados brasileiros, como Acre, Amazonas e Pará (região norte), bem como Pernambuco, com frequências de 60% e 71% para cada região, respectivamente 12 .…”