Oxacilin-resistant Coagulase-negative staphylococci (CoNS) bacteremia in a general hospital at São Paulo city, Brasil

Abstract

In the last decades, coagulase-negative staphylococci (CoNS), especially Staphylococcus epidermidis have become an important cause of bloodstream infections. In addition, rates of methicillin-resistance among CoNS have increased substantially, leading to the use of glicopeptides for therapy. The objective of this study was to evaluate eleven consecutives clinically relevant cases of oxacillin-resistant CoNS bacteremia in a general hospital localized in São Paulo city, Brazil. Five different species were identified by different phenotypic methods, including S. epidermidis (5), S. haemolyticus (3), S. hominis (1), S. warneri (1) and S. cohnii subsp urealyticus (1). A variety of Pulsed Field Gel Electrophoresis profiles was observed by macrorestriction DNA analysis in S. epidermidis isolates, but two of three S. haemolyticus isolates presented the same profile. These data indicated the heterogeneity of the CoNS isolates, suggesting that horizontal dissemination of these microorganisms in the investigated hospital was not frequent. One S. epidermidis and one S. haemolyticus isolates were resistant to teicoplanin and susceptible to vancomycin. The selective pressure due to the use of teicoplanin in this hospital is relevant.

Prevalence of Coagulase Negative Staphylococcus and their Antibiotic Sensitivity Pattern from Various Clinical Samples

“…Maximum number of these isolates (22.22%) have been reported from blood and catheter specimens. Similar reports have also been reported by Cuevas et al and D'Azevedo PA et al 15,16 . However, this is in variance to studies by Roopa C, Asangi YS, Kavitha Y, wherein Staphylococcus epidermidis constituted the predominant species; 50.8%, 44.8% and 46.84% respectively 8,17,18 .…”

Bacteremias por Staphylococcus coagulase negativos oxacilina resistentes em um hospital escola na cidade de Santa Maria, Estado do Rio Grande do Sul

“…Contudo, para esta espécie, as bases moleculares da formação do biofilme ainda não foram totalmente elucidadas. Os níveis elevados de resistência aos antimicrobianos, incluindo a heterorresistência aos glicopeptídeos, compreendem características importantes, que tornam infecções por S. haemolyticus uma ameaça grave 20,25 .…”

“…1,9,[20][21][22][23] . Do ponto de vista epidemiológico, o S. epidermidis tem desenvolvido estratégias interessantes para conquistar o ambiente hospitalar e se transformar em um patógeno notório.…”

Staphylococcus spp. Causatives of Infections and Carrier of blaZ, femA, and mecA Genes Associated with Resistance

“…S. haemolyticus and S. epidermidis have been the most frequently isolated staphylococci in hospital environments and are mainly associated with medical devices [ 26 ]. In one study [ 27 ], there was the identification of 11 isolates of coagulase-negative staphylococci, being 45% S. epidermidis and 27% S. haemolyticus . Of 200 strains of coagulase-negative staphylococci obtained from patients with nosocomial bacteremia in Turkey, 87 corresponded to S. epidermidis and 23 to S. haemolyticus , both of which are more prevalent among 12 other species also isolated [ 28 ].…”