2007
DOI: 10.1590/s1517-83822007000400029
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Abstract: Legionella pneumophila is a pathogenic bacteria associated to aquatic habitat of natural and artificial environments. Clinical cases of legionellosis have been reported in Brazil but there is a lack of information about the incidence and concentration of this bacterium in environmental sources. Thus, the present study was designed to evaluate the occurrence of legionellae in São Paulo city, Brazil, using different methods of detection and identification. Sixty-seven water and biofilm samples from natural reser… Show more

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Cited by 14 publications
(7 citation statements)
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“…In recent years however, L. pneumophila has been prevalent in man-made water systems, including those found in cars (199), water distribution systems and buildings (Center for Disease Control and Prevention, 2016). In these reservoirs, L. pneumophila infects amoeba that upon inhalation act as a “Trojan horse” in humans (200, 201). Due to constant exposure to these water systems in infected infrastructures, cases of Legionellosis are becoming common in human populations (202).…”
Section: Environmental Microbes Turned Human Nightmaresmentioning
confidence: 99%
“…In recent years however, L. pneumophila has been prevalent in man-made water systems, including those found in cars (199), water distribution systems and buildings (Center for Disease Control and Prevention, 2016). In these reservoirs, L. pneumophila infects amoeba that upon inhalation act as a “Trojan horse” in humans (200, 201). Due to constant exposure to these water systems in infected infrastructures, cases of Legionellosis are becoming common in human populations (202).…”
Section: Environmental Microbes Turned Human Nightmaresmentioning
confidence: 99%
“…Legionella species have been isolated from a wide variety of water types, such as potable water of hospital, industries and hotels, ground and surface water and biofilms [53]. Moreover, several authors [1,3,50,54] have described the isolation of Legionella spp.…”
Section: Discussionmentioning
confidence: 99%
“…3,4 Although anterior segment complications related to use of antimicrobial agents have been reported in observational case series, amoebicidal activity of these compounds has improved infection during the management of AK. 8,9,19,28 Due to the aggressive, prolonged, and not uniformly successful treatment currently employed, alternative therapeutic procedures have been proposed for AK. For this reason, the effect of UV-A light associated with a photosensitizer (riboflavin) in the viability of Acanthamoeba trophozoites was evaluated in this study.…”
Section: Discussionmentioning
confidence: 99%
“…Briefly, the amoeba was isolated from corneal tissue by plate-culture procedure onto a Foronda medium covered with avirulent, plasmidless, and heat-inactivated Escherichia coli strain at 25°C. 19 Occurrence of Acanthamoeba sp. was observed after 8 days postinoculation, when cysts and trophozoites were picked up aseptically from nonselective agar medium and transferred to a tissue culture flask containing 5 mL of Neff's broth medium 20 with gentamicin (10 mg/mL) to proceed with axenization process of primary isolate.…”
Section: Methodsmentioning
confidence: 99%