Viral Hepatitis C in a leading Brazilian hospital: epidemiological factors and genotyping

Bezerra, Cristianne Sousa; Lima, José Moreira; Vilar, Janaina L.; Moreira, José Luciano Bezerra; Frota, Cristiane Cunha

doi:10.1590/s1517-83822007000400014

Cited by 12 publications

(8 citation statements)

References 46 publications

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“…e HCV-RNA percentage in HCV antibody-positive patients in our community was 61.1% (124/203). Similar disparities in ratio between HCV antibody-positive and HCV-RNA viral load detectable plasma levels have been reported by others (10,11).…”

Section: Discussionsupporting

confidence: 85%

“…Another important nding of this study is that older patients (>50 years) su ered signi cantly more from HCV-RNA viral load than younger (<50) ones, which is dissimilar to studies conducted in Brazil and the USA (10,12). e reasons for this could be mainly because older patients had a greater probability of undergoing blood transfusions and surgery before 1993.…”

Section: Discussioncontrasting

confidence: 61%

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Most common HCV genotypes in patients from north-eastern Croatia

Perić

Bošnjak

Džijan

et al. 2014

AMA

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Objective. e aims of this study were to determine the HCV-RNA viral load, genotype distribution, risk factors and symptoms of HCV-RNA positive viral load in HCV antibody-positive patients from north-eastern Croatia. Materials and methods. From January 2009 to December 2011, 203 HCV antibody-positive patients (130 men and 73 women; median age 44.5 years) were analyzed for HCV-RNA by the COBAS TaqMan HCV test and genotyped by the Linear Array HCV Genotyping test (both from Roche). All patients completed a structured questionnaire about risk factors and symptoms. Results.e HCV-RNA percentage was 61.1% and was similar for men and women. e HCV-RNA viral load increased with age: while 55% of 20-50 year old patients were HCV-RNA positive, 73% of patients >50 years were positive (p=0.021). Genotype 1 was the most prevalent genotype (79.8%), followed by 3 (12.9%), 4 (6.5%), and 2 (0.8%); genotypes 5 and 6 were not determined. Patients with genotype 1 (median, 50 years) were older than patients with 3 (median, 33.5 years) or 4 (median, 38 years). e blood transfusions performed in Croatian hospitals before 1993 was signi cantly associated with HCV-RNA positive viral load (p<0.05). Conclusion.ese data indicated an elevated prevalence of genotype 1 in elderly HCV-RNA positive patients and it may continue to rise. Using RNA-based detection in HCV positive-antibody patients would allow early detection of HCV in the acute stage of HCV disease and the increased risk of HCV genotyperelated treatment failure.

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Section: Discussionsupporting

confidence: 85%

Section: Discussioncontrasting

confidence: 61%

Most common HCV genotypes in patients from north-eastern Croatia

Perić

Bošnjak

Džijan

et al. 2014

AMA

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“…HCV-RNA was detected in serum samples from 115 out of 154 patients (74.7%). The frequency of HCV-RNA from anti-HCV-positive patients was similar to that reported in other studies (1,2,6,9,11). …”

supporting

confidence: 89%

Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil

Gonzaga¹,

Rodart²,

Reis³

et al. 2008

Braz. J. Microbiol.

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We determined the frequency of hepatitis C virus (HCV) genotypes in anti-HCV seropositive patients in the state of Alagoas, Brazil, by means of nested-reverse transcription-polymerase chain reaction (RT-nested-PCR) followed by restriction fragment length polymorphism (RFLP) of amplified fragments of the 5´NCR. The nested-PCR with genotype-specific primers from the core region was carried out when detection was not possible by the first approach. Detectable HCV-RNA was present in 115 (74.7%) of 154 serum samples. Genotype 1 was the most frequent (77.4%), against 20.9% of genotype 3 and 0.8% of genotype 2. Subtype 1b was predominant (65.2%), followed by subtypes 1a (8.7%), and 3a (6.1%). Coinfection (1a/3a) was detected in 0.8% of the samples. Indeed, there was no significant differences in the prevalence of genotype 1 compared to what has been obtained from anti-HCV seropositive patients from other locations in Brazil. Here we report for the first time the genotype 2 in the state of Alagoas.

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“…Outros métodos de genotipagem são baseados em técnicas sorológicas e moleculares. A reação em cadeia da polimerase (PCR) com métodos de genotipagem incluem o uso de primers para amplificação, análise de hibridização reversa, usando sondas específicas para uma região do VHC e por análises da técnica de restriction fragment length polymorphism (RFLP) 5 .…”

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Caracterização do vírus da hepatite C em pacientes com hepatite crônica: genótipos no Estado do Amazonas, Brasil

Araújo

Almeida

Fraporti

et al. 2011

Rev. Soc. Bras. Med. Trop.

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The genotype of 69 HCV positive patients was determined. An in-house standardized nested-PCR was used to detect HCV RNA. Genotype assignment was based on type-specific motifs on the sequenced amplicons delimited by primers HC11/HC18 from the 5' untranslated region. Results: Of the 69 patients studied, 65.2% were male and 34.8% were female. Genotype 1 showed the greatest prevalence, followed by 3 and 2. Conclusions: These data suggesting that Manaus is the point of arrival of HCV in the State of Amazonas.

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Viral Hepatitis C in a leading Brazilian hospital: epidemiological factors and genotyping

Cited by 12 publications

References 46 publications

Most common HCV genotypes in patients from north-eastern Croatia

Most common HCV genotypes in patients from north-eastern Croatia

Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil

Caracterização do vírus da hepatite C em pacientes com hepatite crônica: genótipos no Estado do Amazonas, Brasil

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