Characterization of alkaline lipase from Fusarium oxysporum and the effect of different surfactants and detergents on the enzyme activity

Abstract

Nowadays, there is a tendency of use of low temperature in laundry cleaning for both environmental and economical reasons, which makes the use of enzymes in detergent products indispensable. Since lipases are efficient catalyst both in solution and at the water-liquid interface, they are potentially suitable for lipid stain removal applications in industrial laundry and household detergents. The effect of different commercial detergents and surfactants on enzymatic activity of lipase from Fusarium oxysporum was observed through p-nitrophenylpalmitate (pNPP) assay. The enzyme was compatible with various ionic and non-ionic surfactants as well as commercial detergents. Lipase activity was strongly inhibited by Sodium Dodecyl Sulphate (SDS), but not by Triton X-100 and Triton X-114. The best assay conditions observed for this lipase were pH 8.0 and 50ºC. The enzyme was stable at alkaline pH and remained 93% of residual activity during 1 h incubation at 60ºC. The highest lipase activity was measured with triglycerides of middle and long chain fatty acids (C8-C18). This enzyme showed a variable specifity/hydrolytic activity towards various fats and oils. All these properties and its resistance towards various surfactants and tolerance to commercial detergents make this lipase a potential additive for detergent formulation.

Optimization of lipase synthesis by Mucor racemosus - Production in a triple impeller bioreactor

“…Tween 20 and 80 had inhibitory effects (54.7-31.4%) on the lipolytic activities at all levels Table 5. These results were similar to those of Prazeres et al (2006) responded to the added amino acid. Glutamic acid had a maximum inhibitory effect (29.4%).…”

A Useful Methodology to Select Lipase-Catalyzed Transesterification Aiming Biodiesel Application

“…On other hand, some authors obtained lipase activity only after 5 days of cultivation (ABBAS et al, 2002;ACIKEL et al, 2011;RAPP, 1995). Investigations of the carbon source effects have been done in order to find good lipase inducers and enhance lipase production (PRAZERES et al, 2006;ANDRADE et al, 2013). RAPP et al (1995) mentioned the use of olive oil added to glucose to produce lipases by F. oxysporum.…”

“…Fusarium oxyporum is well-known specie as good lipase producer (PRAZERES et al, 2006) and improvements on its production could be an important tool to optimize its application (KAUSHIK et al, 2010).…”

Partial purification and biochemical characterization of extracellular lipase from Azospirillum sp. JG3 bacteria

“…Lipase activity assay was carried out by the titrimetric method as described by [10]. The substrate used was 25% olive oil emulsion in 7% Arabic gum solution.…”

“…Effect of surfactants (SDS and Tween 80) and commercial detergents (Rinso, Attack, and SoKlin) on lipase activity were determined according to [10]. 250 μL of enzyme solution was preincubated in 250 μL of surfactants or commercial detergents (solution of 0.1 and 1.0% w/v) for 30 minutes at 30 °C.…”