Aflatoxin M1 in milk by immunoaffinity column cleanup with TLC/HPLC determination

Abstract

During 2002 and 2003, a total of 107 samples of raw, pasteurized and ultrahigh treated temperature (UHT) milk commercialized in the cities of São Paulo and Marília (SP) were analyzed for the presence of aflatoxin M1 (AFM1). AFM1 was detected in 79 (73.8%) of milk samples, ranging from <0.02 to 0.26 μg/L.The samples were analyzed using an immunoaffinity column for cleanup and a thin layer chromatography for determining AFM1. The parameters, such as recovery, repeatibility, detection and quantification limit were evaluated to optimize this method (in-house). Based on spiked samples, the recovery values ranged from 85.83 to 73.86% at levels of 0.010-0.50 μg/L, respectively, and the relative standard deviation for repeatibility ranged from 7.73 to 2.08%. The quantification limit was 0.02 μg/L. The results of some samples analyzed by this method demonstrated a satisfatory correlation when compared with High Performance Liquid Chromatography (HPLC). In conclusion, immunoaffinity column cleanup gave excellent results for recovery, sensibility and sample through put. Despite the high rate of occurrence of AFM1 in samples in both cities, the contamination level could not be considered a serious public health hazard, according to Brazilian legislation.

A limited survey of aflatoxin M1 in milk from Indonesia by ELISA

“…Incidence of AFM1 in another area reported was from São Paulo and Marília (SP). A total of 107 samples of raw, pasteurised and ultrahigh treated temperature (UHT) milk commercialized were analysed and 79 (73.8%) of milk samples, ranging from <0.02 to 0.26 lg/L and 6.5% was contaminated more than 50 ng/L (Shundo & Sabino, 2006). Similar results of AFM1 contamination in milk-based products have also been reported in several European countries.…”

Contamination of UHT milk by aflatoxin M1 in Iran

“…Afatoxin B 1 has a high toxicity rate for animals (Gourama & Bullerman, 1995). Although AFM 1 , the hydroxylated metabolite of AFB 1 , is less carcinogenic and mutagenic than AFB 1 , it exhibits a high level of genotoxic activity and certainly represents a health risk because of its possible accumulation and linkage to DNA (Shundo & Sabino, 2006). The occurrence of AFM 1 in milk and milk products is a public health concern because the International Agency for Research on Cancer (IARC) of WHO included AFB 1 as primary and AFM 1 as secondary groups of carcinogenic compounds (IARC, 1993;Maurice, 2002).…”

Safety and Public Health Hazards Associated with Egyptian Soft Cheese Consumption

“…The identification of isolated mould genera were carried out based on their micro morphological properties (Pitt and Hocking, 2009). Qualitative and quantitative estimation of aflatoxins (Shundo andSabino, 2006), and("EC" (2006). White soft cheese is widely consumed by the Egyptian population.…”