Cytotoxicity of subfractions and compounds from Polymnia sonchifolia

Fernandes, Maria Judite Bittencourt; Limas, Caroline; Rossi, Maria Helena Wagner; Goncales, Edlayne; Simoni, Isabela Cristina

doi:10.1590/s1517-83822005000400006

Cited by 5 publications

(4 citation statements)

References 10 publications

(10 reference statements)

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“…A number of methods including colorimetric and fluorometric are currently used in the fields of toxicology and pharmacology to determine cellular viability following in vitro exposure to compounds (11). Each method has specific advantages, disadvantages and limitations and the suitable method is the users' choice (12). To select the appropriate solvent with suitable concentrations, the most standardized and validated test to estimate the cytotoxicity of compounds towards cells is MTT test.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

Jamalzadeh¹,

Ghafoori²,

Sariri³

et al. 2016

Avicenna J Med Biochem

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Background: Organic solvents are widely used in cell biology experiments. Despite increasing the solubility, they have some moderate toxic effects. Therefore, selecting the appropriate solvent along with the use of suitable concentration insures the accuracy and reliability of experimental results. Objectives: The current study aimed to examine the cytotoxic effects of some organic solvents on various cell models including MCF-7, RAW-264.7 and human umbilical vein endothelial cells (HUVEC). Materials and Methods:To evaluate the cytotoxicity effect of common organic solvents on the MCF-7, RAW-264.7 and HUVEC cells, multi-table tournament (MTT) colorimetric assay, the widely used and validated cytotoxicity test was applied. For this purpose, the selected cells were treated with different concentrations (0, 0.1%, 0.5%, 1%, 1.5%, 2%, 3% and 5% v/v) of four most commonly used organic solvents (acetone, ethanol, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) and then subjected to MTT experiment. Results: According to the obtained results, the cytotoxicity increased significantly with increasing the concentration of all four solvents compared to that of the control group. Studies with MCF-7, RAW-264.7 and HUVEC suggested that acetone, ethanol and DMSO at concentrations of 0.1% and 0.5%, had little or no toxicity, whereas higher concentrations inhibited the growth of all three cells. Compared with other three solvents, DMF displayed rather greater toxicity. Based on the results, proliferation of MCF-7, RAW-264.7 and HUVEC cells were inhibited by all used organic solvents, dose dependently. Conclusions: Thus, the background experimental error can be reduced remarkably by maximal concentration of 0.5% ethanol, acetone and DMSO and 0.1% DMF in the final treatment medium.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

Jamalzadeh¹,

Ghafoori²,

Sariri³

et al. 2016

Avicenna J Med Biochem

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“…Nonetheless, TK activity is necessary for the full expression of virulence in vivo and its deletion from HSV-1, BoHV-1 and pseudorabies virus (PRV) genomes is associated with significant reduction in virulence (1,3,16,27). Therefore, TK deletion has become an attractive target for gene manipulation of herpesviruses aiming to obtain attenuated viruses for vaccine use (3,10,17,26,28).…”

Section: Introductionmentioning

confidence: 99%

Selection and characterization of brivudin resistant bovine herpesvirus type 5

Brum¹,

Santos²,

Weiblen³

et al. 2010

Braz. J. Microbiol.

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Bovine herpesvirus type 5 (BoHV-5) is the agent of meningoencephalitis, an important disease of cattle in South America. The neuropathogenesis of BoHV-5 infection is poorly understood and most previous research focused on the role of envelope glicoproteins in neurovirulence. Thymidine kinase (TK) is a viral enzyme necessary for virus replication in neurons and, therefore, represents a potential target for virus attenuation. The selection and characterization of BoHV-5 variants resistant to the nucleoside analog brivudin (BVDU), which selects TK-defective viruses is here described. Several BVDU-resistant clones were obtained after multiple passages in tissue culture in the presence of BVDU and one clone (BoHV-5/R-27) was further characterized. The selected clone replicated to similar titers and produced plaques with similar size and morphology to those of wild-type virus (SV507/99). The genetic stability of the resistant virus was demonstrated after ten passages in cell culture in the absence of the drug. Moreover, the drugresistant virus showed reduced virulence in a rabbit model: virus inoculation in four rabbits did not result in disease, in contrast with 75% morbidity (3/4) and 50% mortality (2/2) among rabbits inoculated with the parental virus. These results demonstrate that BoHV-5 is sensitive to BVDU and that drug-resistant mutants can be readily selected upon BVDU treatment. BVDU-resistant mutants, likely defective in TK, retained their ability to replicate in tissue culture yet were attenuated for rabbits. This strategy to obtain TK-defective BoHV-5 may be useful to study the role of TK in BoHV-5 neuropathogenesis and for vaccine development.

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“…In fact, several phytochemical and pharmacological studies (Aybar et al, 2001;Genta et al, 2010;Valentová et al, 2005) of yacon leaves have been conducted in the recent decades. Although there is sufficient evidence of their potential activity in the treatment of hyperglycaemia and its complications, there is still disagreement about their safe use and the appropriate therapeutic dose (de Oliveira et al, 2011;Fernandes et al, 2005;Genta et al, 2010;Siriwan et al, 2011). In the current study, we demonstrated that both a 10% decoction of yacon leaves and enhydrin have a safety margin in Wistar rats at doses in which was demonstrated hypoglycaemic effects.…”

Section: Discussionmentioning

confidence: 52%

“…Despite the traditional use of decoction yacon leaves, in the literature there are few studies that evaluate the toxic potential of both yacon leaves extract and pure compounds, and these are contradictory (de Oliveira et al, 2011;Fernandes et al, 2005;Genta et al, 2010;Ogose et al, 2009;Siriwan et al, 2011). Thus, the present study was performed to evaluate the subchronic toxicity profile of both the 10% decoction and pure enhydrin in rats.…”

Section: Discussionmentioning

confidence: 95%