Detection of mixed microbial biofilms on central venous catheters removed from Intensive care Unit Patients

Storti, Anisio; Pizzolitto, Antônio Carlos; Pizzolitto, Elisabeth Loshchagin

doi:10.1590/s1517-83822005000300013

Cited by 18 publications

(15 citation statements)

References 26 publications

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“…In a study regarding to microbial biofilm detection on venous catheters removed from ICU patients, Stort et al (65) used scanning electron microscopy and reported that the extracellular biofilm matrix appears as an amorphous material on the catheter surface, similar to the observation of Donlan (29).…”

Section: Slime Production By Coagulase-negative Staphylococci and Itsmentioning

confidence: 75%

Bacterial biofilms with emphasis on coagulase-negative staphylococci

Oliveira¹,

Cunha²

2008

J. Venom. Anim. Toxins incl. Trop. Dis

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In addition to their capacity to attach to surfaces, various groups of microorganisms also produce an extracellular polymeric substance known as "slime". This slime forms a thin layer around cells known as biofilm. Thus, biofilm structure comprises bacterial cells and an extracellular polymeric substance. It also presents a defined architecture, providing the microorganisms with an excellent protective environment and favoring the exchange of genetic material between cells as well as intercellular communication. The ability to produce biofilm is observed in a large group of bacteria, including coagulase-negative staphylococci (CNS) which are the predominant microorganisms of normal skin flora and have been implicated as the causative agents of hospital infections. Bacteremia caused by these agents is common in immunodepressed persons, in patients with cancer, in adult and neonatal intensive care units (ICU) and in patients using catheters or other prosthetic devices. The pathogenicity of CNS infections is probably related to the production of slime, which adheres preferentially to plastic and smooth surfaces, forming a biofilm that protects against attacks from the immune system and against antibiotic treatment, a fact hindering the eradication of these infections. The main objective of the present review was to describe basic and genetic aspects of biofilm formation and methods for its detection, with emphasis on biofilm creation by CNS and its relationship with diseases caused by these microorganisms which are becoming increasingly more frequent in the hospital environment.

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Section: Slime Production By Coagulase-negative Staphylococci and Itsmentioning

confidence: 75%

Bacterial biofilms with emphasis on coagulase-negative staphylococci

Oliveira¹,

Cunha²

2008

J. Venom. Anim. Toxins incl. Trop. Dis

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“…20,21 Small sample size could be an analytical problem; however, our sample size is similar to other studies. 18,19,22,23 Only multicenter or large retrospective studies have larger samples. [24][25][26] We observed that the site of implant may be related to a greater or lesser predisposition to infection.…”

Section: Discussionmentioning

confidence: 99%

Biofilms, Infection, and Parenteral Nutrition Therapy

Machado

Suen

Figueiredo

et al. 2009

J Parenter Enteral Nutr

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Parenteral nutrition therapy is used in patients with a contraindication to the use of the gastrointestinal tract, and infection is one of its frequent and severe complications. The objective of the present study was to detect the presence of biofilms and microorganisms adhering to the central venous catheters used for parenteral nutrition therapy by scanning electron microscopy. Thirty-nine central venous catheters belonging to patients with clinical signs of infection (G1) and asymptomatic patients (G2) and patients receiving central venous catheters for clinical monitoring (G3) were analyzed by semiquantitative culture and scanning electron microscopy. The central venous catheters of G1 presented more positive cultures than those of G2 and G3 (81% vs 50% and 0%, respectively). However, biofilms were observed in all catheters used and 55% of them showed structures that suggested central venous catheters colonization by microorganisms. Approximately 53% of the catheter infections evolved with systemic infection confirmed by blood culture. The authors conclude that the presence of a biofilm is frequent and is an indicator of predisposition to infection, which may even occur in patients who are still asymptomatic.

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“…Scanning electron microscopy (SEM) studies, using the method of Storti et al (2005), were carried out on P. aeruginosa or C. tropicalis for the lock concentrations of 64, 256 or 1024 mg/L and compared to the antibiotic free PBS.…”

Section: Scanning Electron Microscopy (Sem) Studiesmentioning

confidence: 99%