2004
DOI: 10.1590/s1517-83822004000300013
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Abstract: The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the highest produ… Show more

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Cited by 3 publications
(2 citation statements)
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References 18 publications
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“…The knowledge about bacterial metabolism is essential for the development of an efficient process [ 22 ]. For this purpose, the fed-batch operation could be better than the conventional batch operation, especially when concentration variations of one or more nutrients affect the yield or productivity of the desired metabolite [ 23 , 24 ]. Therefore, to prevent the depletion of glucose in the medium during cultivations, a pulse of glucose could recover the cellular growth and, thus, an increase is expected in the PS production.…”
Section: Introductionmentioning
confidence: 99%
“…The knowledge about bacterial metabolism is essential for the development of an efficient process [ 22 ]. For this purpose, the fed-batch operation could be better than the conventional batch operation, especially when concentration variations of one or more nutrients affect the yield or productivity of the desired metabolite [ 23 , 24 ]. Therefore, to prevent the depletion of glucose in the medium during cultivations, a pulse of glucose could recover the cellular growth and, thus, an increase is expected in the PS production.…”
Section: Introductionmentioning
confidence: 99%
“…The M. bovis (BCG) Moreau strain was kindly supplied by the Ataulpho de Paiva Foundation (FAP). The BCG vaccine was utilised at two multiplicity of infection (MOI) of bacillus/THP-1 derived macrophages, 1/1 and 5/1, and the mycobacteria were obtained from two different media, Sauton medium at 37ºC, ID vaccine ( Leal et al 2004 ) and IVM medium, oral vaccine. Cells from the human THP-1 derived monocytes (acute monocytic leukaemia) were cultivated in a culture flask in RPMI medium containing 10% heat-inactivated foetal calf serum (FCS) (Cutilab cat.…”
mentioning
confidence: 99%