Antimutagenic action of Lentinula edodes and Agaricus blazei on Aspergillus nidulans conidia

Abstract

The antimutagenic effect of the mushrooms Lentinula edodes and Agaricus blazei was studied on conidia of Aspergillus nidulans when exposed to short wave ultraviolet light. Two strains of A. nidulans were used. For the preparation of the extracts, the fresh mushrooms were left in aqueous infusion for 12 hours and heated in a water bath for 15 min at 100ºC, and then the material was filtered. The dehydrated mushrooms were left in aqueous infusion for 12 hours and to filtrated. Both filtrates were used as extracts. A. nidulans conidia were incubated for three hours in water and in mushroom extracts and only after were exposed to UV light (pretreatment). A. nidulans conidia were suspended in water and in mushroom extracts and immediately submitted to UV light (post-treatment). Conidial suspension in water and in mushroom extracts but without exposure to the mutagenic agent were used as controls. After mutagenic treatment, it was observed an increase in the survival rate of the A. nidulans and a decrease in the percentage of morphologic mutants on conidia treated with mushroom extracts. Our results demonstrated the radioprotective and antimutagenic effect of L. edodes and A. blazei mushrooms on eukaryotic cells when exposed to UV radiation.

Photoprotective and Antimutagenic Activity of Agaricus subrufescens Basidiocarp Extracts

“…These results are in accordance with other authors who also report no toxicity of A. subrufescens basidiocarp extracts [10,14,[19][20][21][22]26].…”

“…blazei Murrill sensu Heinemann [12]; =A. brasiliensis Wasser et al [28]) has antimutagenic [23], antitumor [5,19], immune stimulating [10], antioxidant [20], anti-inflammatory [21], and UV protecting [26] activities.…”

Antimutagenicity and antigenotoxicity of Aloe arborescens Miller and Aloe barbadensis Miller in Aspergillus nidulans and Wistar rats

“…However, the 4% concentration reduced the frequency of spontaneous mutations compared to the Control, which suggests the mucilage of the leaves may possess antioxidant effects. This system-test is efficient and has been used in other investigations of phytotherapeutic products such as the study by Rodrigues et al (2003) on the mushroom of the Sun (Agaricus blazei) and Souza-Paccola et al (2004) who investigated the Lentinula edodes and A. blazei mushrooms.…”

Carbon-to-nitrogen ratios for Agaricus brasiliensis on the axenic method

“…Agaricus brasiliensis Wasser et al (A. blazei Murrill sensu Heinemann), , also denoted A. subrufescens (KERRIGAN, 2005), is a fungus native to Brazil (BRAGA et al, 1998;COLAUTO et al, 2002;DIAS et al, 2004;EIRA, 2003; and it has been reported many therapeutic properties (KIM et al, 2005;KIMURA et al, 2004;MIZUNO, 2002;OSHIMAN et al, 2002;RODRIGUES et al, 2003;SOUZA-PACCOLA et al, 2004). It has been produced in Brazil for commercial purposes since 1990 and 90% of its current production is exported to Japan (EIRA, 2003).…”