Use of molecular approach to verify the influence of a eutrophic lagoon in the nearby ocean's bacterioplankton communities

Taketani, Rodrigo Gouvêa; Araújo, Fábio Vieira de; Weerelt, Margaretha Denise Maria van; Sanchez-Nunez, Maria Leonisa; Aniszewski, Erick; Rosado, Alexandre Soares

doi:10.1590/s1517-83822003000500017

Cited by 2 publications

(3 citation statements)

References 4 publications

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“…The view of eutrophication is biased from observations in few systems (eg., Taketani et al , 2003; Araujo and Hagler, 2011). This scientific discipline is still very young to marine systems and a broader view of costal eutrophication will consider how anthropogenic nutrient enrichment interact with other stressors such as species translocation, habitat loss, fishing, inputs of toxic contaminants, manipulation of freshwater flows, aquaculture, and climate change.…”

Section: Resultsmentioning

confidence: 99%

Yearly variation of bacterial production in the Arraial do Cabo protection area (Cabo Frio upwelling region): an evidence of anthropogenic pressure

Coelho-Souza¹,

Pereira²,

Coutinho³

et al. 2013

Braz. J. Microbiol.

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Arraial do Cabo is where upwelling occurs more intensively on the Brazilian coast. Although it is a protection area it suffers anthropogenic pressure such as harbor activities and sporadic sewage emissions. Short-time studies showed a high variability of bacterial production (BP) in this region but none of them evaluated BP during long periods in a large spatial scale including stations under different natural (upwelling and cold fronts) and anthropogenic pressures. During 2006, we sampled surface waters 10 times (5 in upwelling and 5 in subsidence periods) in 8 stations and we measured BP, temperature as well as the concentrations of inorganic nutrients, pigments and particulate organic matter (POM). BP was up to 400 times higher when sewage emissions were observed visually and it had a positive correlation with ammonia concentrations. Therefore, in 2007, we did two samples (each during upwelling and subsidence periods) during sewage emissions in five stations under different anthropogenic pressure and we also measured particles abundance by flow cytometry. The 12 samples in the most impacted area confirmed that BP was highest when ammonia was higher than 2 μM, also reporting the highest concentrations of chlorophyll a and suspended particles. However, considering all measured variables, upwelling was the main disturbing factor but the pressure of fronts should not be neglected since it had consequences in the auto-heterotrophic coupling, increasing the concentrations of non fluorescent particles and POM. Stations clustered in function of natural and anthropogenic pressures degrees and both determined the temporal-spatial variability.

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Section: Resultsmentioning

confidence: 99%

Yearly variation of bacterial production in the Arraial do Cabo protection area (Cabo Frio upwelling region): an evidence of anthropogenic pressure

Coelho-Souza¹,

Pereira²,

Coutinho³

et al. 2013

Braz. J. Microbiol.

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“…Extraction, PCR, and DGGE analysis of community DNA A 0.22-lm membrane containing material obtained after the filtration of 300 mL of water collected from each sampling location was cut in half, and one of the pieces was then cut into small pieces for DNA extraction with the FastDNA Ò SPIN Kit for Soil (BIO101, California, USA), as described by Taketani et al (2003).…”

Section: Bacterial Cell Countsmentioning

confidence: 99%

“…Furtado et al 2001), generally using only cultivation-dependent or other conventional methods. As far as we know, there has been only one report of the use of molecular tools to study bacterial communities in urban lagoons (Taketani et al 2003). Several papers have demonstrated the influence of imported populations on an environment using molecular methods (Bergström and Jansson 2000;Crump et al 1999;Lindströn 1998;Schauer et al 2000), but all of them analysed temperate climate environments, which present different characteristics than tropical ones.…”

Section: Introductionmentioning

confidence: 99%

Influence of the bacterioplankton community of a tropical eutrophic lagoon on the bacterial community of its neighbouring ocean

Taketani

Araújo

Cannavan

et al. 2010

World J Microbiol Biotechnol

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The Rodrigo de Freitas lagoon (RFL) is a tropical eutrophic coastal ecosystem located in the urban area of Rio de Janeiro, Brazil. This environment consists of freshwater but has communication with the ocean through a channel (Jardim de Alah's Channel). The aim of this study was to evaluate the influence of lagoon water on the nearby ocean using molecular and traditional microbiological methods. We hypothesised that due to the eutrophic low-salinity environment, the bacterioplankton community from the RFL would have a native ''brackish'' composition influenced by both freshwater and marine phylotypes, and that bacterial phylotypes of this community would be detected in oceanic samples closer to the channel between the lagoon and the ocean. The cultivation and microscopy experiments clearly showed this influence. Bacterial cell counts revealed that the greater amounts of bacterial cells present in the lagoon increased the observed values seen at oceanic stations near the channel. The Denaturing gradient gel eletrophoresis community profiles also showed a clear influence of Rodrigo de Freitas lagoon waters on the adjacent beaches. The band patterns found for the stations near the channel showed that these communities were mixtures of the communities of the lagoon and sea, and as the distance from the channel increased, the samples became more similar to ocean bacterial communities. A 16S rRNA gene clone library was constructed using a sample acquired from the connection point between the lagoon and the ocean. Around 52% of the sequences in the library showed similarity to the genus Proteobacteria (1% Alpha, 21% Beta, 19% Gamma and 29% unclassified Proteobacteria), and the second most abundant genus was Bacteroidetes, with 15% of the total clones. The results showed that the structure of the bacterial community had both freshwater and marine characteristics.

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Use of molecular approach to verify the influence of a eutrophic lagoon in the nearby ocean's bacterioplankton communities

Cited by 2 publications

References 4 publications

Yearly variation of bacterial production in the Arraial do Cabo protection area (Cabo Frio upwelling region): an evidence of anthropogenic pressure

Yearly variation of bacterial production in the Arraial do Cabo protection area (Cabo Frio upwelling region): an evidence of anthropogenic pressure

Influence of the bacterioplankton community of a tropical eutrophic lagoon on the bacterial community of its neighbouring ocean

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