2002
DOI: 10.1590/s1517-83822002000400001
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Abstract: Inhibitors of plant proteases can regulate the hydrolysis of proteins inside the cells and also participate in the mechanisms of plant defense against herbivore insects and pathogens. Here, we demonstrated that seeds of Eucalyptus urophylla exhibit activities of trypsin and papain inhibitors, two proteases commonly found in living cells. Low amounts of proteins of the crude protein extract of seeds and fractions partially purified by gel filtration, with inhibitory activity against trypsin, inhibited in vitro … Show more

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Cited by 16 publications
(12 citation statements)
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References 7 publications
(4 reference statements)
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“…Again, the high antiprotease activity recorded in this study agrees with previous work involving feeding with herbs (Vasudeva & Chakrabati 2004). In particular, inhibition of trypsin may regulate the hydrolysis of protein in vivo and thus up‐regulates the mechanism of immune defence against pathogens (Tremacoldi & Pascholati 2002). Increases in serum total protein, albumin and globulin is similarly in line with previous work involving immunostimulants, namely chitosan and β‐glucan (Siwicki 1989) and yeast RNA (Choudhury, Pal, Sahu, Kumar, Das & Mukherjee 2005).…”
Section: Discussionsupporting
confidence: 89%
“…Again, the high antiprotease activity recorded in this study agrees with previous work involving feeding with herbs (Vasudeva & Chakrabati 2004). In particular, inhibition of trypsin may regulate the hydrolysis of protein in vivo and thus up‐regulates the mechanism of immune defence against pathogens (Tremacoldi & Pascholati 2002). Increases in serum total protein, albumin and globulin is similarly in line with previous work involving immunostimulants, namely chitosan and β‐glucan (Siwicki 1989) and yeast RNA (Choudhury, Pal, Sahu, Kumar, Das & Mukherjee 2005).…”
Section: Discussionsupporting
confidence: 89%
“…Extraction of proteins from 100-to 150-mg samples and analysis of PI activity followed a previously described method (Tremacoldi and Pascholati 2002;Sarmento et al…”
Section: Proteinase Inhibitor (Pi) Activity Assaymentioning
confidence: 99%
“…We tested the clones on which Wrst instar larvae survived best (susceptible clones) and those on which survival was worst (resistant clones) to determine if diVerences were due to the activity of digestibility reducers, similar perhaps to a trypsin inhibitor found in seeds of E. urophylla (Tremacoldi and Pascholati 2002). Although the column separation successfully extracted leaf protein, extracts from resistant and susceptible clones did not diVer in their ability to inhibit proteolytic activity.…”
Section: Protease Inhibition and Condensed Tannin Content Assaysmentioning
confidence: 99%